ACIN1, CPTAC-1342 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ACIN1	ACINUS, KIAA0670

Sequence Length (AA)	Molecular Weight (Da)
1341	151862

Protein Name
Apoptotic chromatin condensation inducer in the nucleus

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWRRKHPRTS	GGTRGVLSGN	RGVEYGSGRG	HLGTFEGRWR	KLPKMPEAVG
60	70	80	90	100
TDPSTSRKMA	ELEEVTLDGK	PLQALRVTDL	KAALEQRGLA	KSGQKSALVK
110	120	130	140	150
RLKGALMLEN	LQKHSTPHAA	FQPNSQIGEE	MSQNSFIKQY	LEKQQELLRQ
160	170	180	190	200
RLEREAREAA	ELEEASAESE	DEMIHPEGVA	SLLPPDFQSS	LERPELELSR
210	220	230	240	250
HSPRKSSSIS	EEKGDSDDEK	PRKGERRSSR	VRQARAAKLS	EGSQPAEEEE
260	270	280	290	300
DQETPSRNLR	VRADRNLKTE	EEEEEEEEEE	EDDEEEEGDD	EGQKSREAPI
310	320	330	340	350
LKEFKEEGEE	IPRVKPEEMM	DERPKTRSQE	QEVLERGGRF	TRSQEEARKS
360	370	380	390	400
HLARQQQEKE	MKTTSPLEEE	EREIKSSQGL	KEKSKSPSPP	RLTEDRKKAS
410	420	430	440	450
LVALPEQTAS	EEETPPPLLT	KEASSPPPHP	QLHSEEEIEP	MEGPAPAVLI
460	470	480	490	500
QLSPPNTDAD	TRELLVSQHT	VQLVGGLSPL	SSPSDTKAES	PAEKVPEESV
510	520	530	540	550
LPLVQKSTLA	DYSAQKDLEP	ESDRSAQPLP	LKIEELALAK	GITEECLKQP
560	570	580	590	600
SLEQKEGRRA	SHTLLPSHRL	KQSADSSSSR	SSSSSSSSSR	SRSRSPDSSG
610	620	630	640	650
SRSHSPLRSK	QRDVAQARTH	ANPRGRPKMG	SRSTSESRSR	SRSRSRSASS
660	670	680	690	700
NSRKSLSPGV	SRDSSTSYTE	TKDPSSGQEV	ATPPVPQLQV	CEPKERTSTS
710	720	730	740	750
SSSVQARRLS	QPESAEKHVT	QRLQPERGSP	KKCEAEEAEP	PAATQPQTSE
760	770	780	790	800
TQTSHLPESE	RIHHTVEEKE	EVTMDTSENR	PENDVPEPPM	PIADQVSNDD
810	820	830	840	850
RPEGSVEDEE	KKESSLPKSF	KRKISVVSAT	KGVPAGNSDT	EGGQPGRKRR
860	870	880	890	900
WGASTATTQK	KPSISITTES	LKSLIPDIKP	LAGQEAVVDL	HADDSRISED
910	920	930	940	950
ETERNGDDGT	HDKGLKICRT	VTQVVPAEGQ	ENGQREEEEE	EKEPEAEPPV
960	970	980	990	1000
PPQVSVEVAL	PPPAEHEVKK	VTLGDTLTRR	SISQQKSGVS	ITIDDPVRTA
1010	1020	1030	1040	1050
QVPSPPRGKI	SNIVHISNLV	RPFTLGQLKE	LLGRTGTLVE	EAFWIDKIKS
1060	1070	1080	1090	1100
HCFVTYSTVE	EAVATRTALH	GVKWPQSNPK	FLCADYAEQD	ELDYHRGLLV
1110	1120	1130	1140	1150
DRPSETKTEE	QGIPRPLHPP	PPPPVQPPQH	PRAEQREQER	AVREQWAERE
1160	1170	1180	1190	1200
REMERRERTR	SEREWDRDKV	REGPRSRSRS	RDRRRKERAK	SKEKKSEKKE
1210	1220	1230	1240	1250
KAQEEPPAKL	LDDLFRKTKA	APCIYWLPLT	DSQIVQKEAE	RAERAKEREK
1260	1270	1280	1290	1300
RRKEQEEEEQ	KEREKEAERE	RNRQLEREKR	REHSRERDRE	RERERERDRG
1310	1320	1330	1340	1341
DRDRDRERDR	ERGRERDRRD	TKRHSRSRSR	STPVRDRGGR	R

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1342 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACIN1
Peptide Modified Sequence: KIS[+80.0]VVSATK
Modification Type: Phospho (ST)
Protein - Site of Modification: 825
Peptide - Site of Modification: 3
Peptide Start: 823
Peptide End: 831

CPTAC ID: CPTAC-1342
Peptide Molecular Mass: 1,011.5366
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel

CPTAC-1342: ACIN1. KIS[+80.0]VVSATK	CPTAC-1343: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPS[+80.0]PR	CPTAC-1344: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPSPR
CPTAC-1345: ARAF. QHEAPSNRPLNELLTPQGPS[+80.0]PR	CPTAC-1346: CIC. AILGS[+80.0]YR	CPTAC-1347: EGFR. ELVEPLT[+80.0]PSGEAPNQALLR
CPTAC-1348: GLYR1. KLS[+80.0]LSEGK	CPTAC-1349: GTF2I. GREFS[+80.0]FEAWNAK	CPTAC-1350: HMGN1. KVS[+80.0]SAEGAAKEEPK
CPTAC-1351: HSPB1. GPS[+80.0]WDPFRDWYPHSR	CPTAC-1352: MAPK1. VADPDHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1353: MAPK1. VADPDHDHTGFLT[+80.0]EYVATR
CPTAC-1354: MAPK1. VADPDHDHTGFLTEY[+80.0]VATR	CPTAC-1355: MAPK14. HTDDEMT[+80.0]GYVATR	CPTAC-1356: MAPK14. HTDDEMTGY[+80.0]VATR
CPTAC-1357: MAPK3. IADPEHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1358: MAPK3. IADPEHDHTGFLT[+80.0]EYVATR	CPTAC-1359: MAPK3. IADPEHDHTGFLTEY[+80.0]VATR
CPTAC-1360: MTOR. KLHVS[+80.0]TINLQK	CPTAC-1361: MTOR. LHVS[+80.0]TINLQK	CPTAC-1362: PBRM1. TYS[+80.0]QDC[+57.0]SFK
CPTAC-1363: PHIP. AQS[+80.0]YDIQAWKK	CPTAC-1364: PRKD2. LGTSES[+80.0]LPC[+57.0]TAEELSR	CPTAC-1365: RBM7. SFS[+80.0]SPENFQR
CPTAC-1366: SHC1. ELFDDPSY[+80.0]VNVQNLDK	CPTAC-1367: SMARCA4. EVDYSDS[+80.0]LTEKQWLK	CPTAC-1368: STMN1. ASGQAFELILS[+80.0]PR
CPTAC-1369: SYNPO2. SLS[+80.0]LPGR	CPTAC-1370: UBE2J1. QIS[+80.0]FKAEVNSSGK	CPTAC-1371: ZNF638. NYQSQADIPIRS[+80.0]PFGIVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	7.1	5.8	4.6	4.8	10.5	11.3	8.6	12	12.2	15	15	15
y7-98 (1+)	6.2	4.1	8.7	7.5	10.9	14	9.7	11.6	16.5	15	15	15
y8-98 (1+)	6.8	5	8.6	7.8	11.3	13.7	10.3	12.4	16.2	15	15	15
sum	5.3	3.4	7	6.1	10.1	12.6	8.1	10.7	14.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3629 Collapse assay details

Data source: Panorama

Official Gene Symbol: Acin1
Peptide Sequence: GLLVDRPSETK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1096
Peptide End: 1106

CPTAC ID: non-CPTAC-3629
Peptide Molecular Mass: 1,213.6667
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers