Overview Data source: UniProt

Official Gene Symbol Other Aliases
ACIN1 ACINUS,KIAA0670
Sequence Length (AA) Molecular Weight (Da)
1341 151862
Protein Name
Apoptotic chromatin condensation inducer in the nucleus
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWRRKHPRTS GGTRGVLSGN RGVEYGSGRG HLGTFEGRWR KLPKMPEAVG
60 70 80 90 100
TDPSTSRKMA ELEEVTLDGK PLQALRVTDL KAALEQRGLA KSGQKSALVK
110 120 130 140 150
RLKGALMLEN LQKHSTPHAA FQPNSQIGEE MSQNSFIKQY LEKQQELLRQ
160 170 180 190 200
RLEREAREAA ELEEASAESE DEMIHPEGVA SLLPPDFQSS LERPELELSR
210 220 230 240 250
HSPRKSSSIS EEKGDSDDEK PRKGERRSSR VRQARAAKLS EGSQPAEEEE
260 270 280 290 300
DQETPSRNLR VRADRNLKTE EEEEEEEEEE EDDEEEEGDD EGQKSREAPI
310 320 330 340 350
LKEFKEEGEE IPRVKPEEMM DERPKTRSQE QEVLERGGRF TRSQEEARKS
360 370 380 390 400
HLARQQQEKE MKTTSPLEEE EREIKSSQGL KEKSKSPSPP RLTEDRKKAS
410 420 430 440 450
LVALPEQTAS EEETPPPLLT KEASSPPPHP QLHSEEEIEP MEGPAPAVLI
460 470 480 490 500
QLSPPNTDAD TRELLVSQHT VQLVGGLSPL SSPSDTKAES PAEKVPEESV
510 520 530 540 550
LPLVQKSTLA DYSAQKDLEP ESDRSAQPLP LKIEELALAK GITEECLKQP
560 570 580 590 600
SLEQKEGRRA SHTLLPSHRL KQSADSSSSR SSSSSSSSSR SRSRSPDSSG
610 620 630 640 650
SRSHSPLRSK QRDVAQARTH ANPRGRPKMG SRSTSESRSR SRSRSRSASS
660 670 680 690 700
NSRKSLSPGV SRDSSTSYTE TKDPSSGQEV ATPPVPQLQV CEPKERTSTS
710 720 730 740 750
SSSVQARRLS QPESAEKHVT QRLQPERGSP KKCEAEEAEP PAATQPQTSE
760 770 780 790 800
TQTSHLPESE RIHHTVEEKE EVTMDTSENR PENDVPEPPM PIADQVSNDD
810 820 830 840 850
RPEGSVEDEE KKESSLPKSF KRKISVVSAT KGVPAGNSDT EGGQPGRKRR
860 870 880 890 900
WGASTATTQK KPSISITTES LKSLIPDIKP LAGQEAVVDL HADDSRISED
910 920 930 940 950
ETERNGDDGT HDKGLKICRT VTQVVPAEGQ ENGQREEEEE EKEPEAEPPV
960 970 980 990 1000
PPQVSVEVAL PPPAEHEVKK VTLGDTLTRR SISQQKSGVS ITIDDPVRTA
1010 1020 1030 1040 1050
QVPSPPRGKI SNIVHISNLV RPFTLGQLKE LLGRTGTLVE EAFWIDKIKS
1060 1070 1080 1090 1100
HCFVTYSTVE EAVATRTALH GVKWPQSNPK FLCADYAEQD ELDYHRGLLV
1110 1120 1130 1140 1150
DRPSETKTEE QGIPRPLHPP PPPPVQPPQH PRAEQREQER AVREQWAERE
1160 1170 1180 1190 1200
REMERRERTR SEREWDRDKV REGPRSRSRS RDRRRKERAK SKEKKSEKKE
1210 1220 1230 1240 1250
KAQEEPPAKL LDDLFRKTKA APCIYWLPLT DSQIVQKEAE RAERAKEREK
1260 1270 1280 1290 1300
RRKEQEEEEQ KEREKEAERE RNRQLEREKR REHSRERDRE RERERERDRG
1310 1320 1330 1340 1341
DRDRDRERDR ERGRERDRRD TKRHSRSRSR STPVRDRGGR R

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1342 Collapse assay details

Data source: Panorama

Official Gene Symbol
ACIN1
Peptide Modified Sequence
KIS[+80.0]VVSATK
Modification Type
Phospho (ST)
Protein - Site of Modification
825
Peptide - Site of Modification
3
Peptide Start
823
Peptide End
831
CPTAC ID
CPTAC-1342
Peptide Molecular Mass
1,011.5366
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 7.1 5.8 4.6 4.8 10.5 11.3 8.6 12 12.2 15 15 15
y7-98 (1+) 6.2 4.1 8.7 7.5 10.9 14 9.7 11.6 16.5 15 15 15
y8-98 (1+) 6.8 5 8.6 7.8 11.3 13.7 10.3 12.4 16.2 15 15 15
sum 5.3 3.4 7 6.1 10.1 12.6 8.1 10.7 14.4 15 15 15

Additional Resources and Comments