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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PBRM1 BAF180, PB1
Sequence Length (AA) Molecular Weight (Da)
1689 192948
Protein Name
Protein polybromo-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSKRRRATS PSSSVSGDFD DGHHSVSTPG PSRKRRRLSN LPTVDPIAVC
60 70 80 90 100
HELYNTIRDY KDEQGRLLCE LFIRAPKRRN QPDYYEVVSQ PIDLMKIQQK
110 120 130 140 150
LKMEEYDDVN LLTADFQLLF NNAKSYYKPD SPEYKAACKL WDLYLRTRNE
160 170 180 190 200
FVQKGEADDE DDDEDGQDNQ GTVTEGSSPA YLKEILEQLL EAIVVATNPS
210 220 230 240 250
GRLISELFQK LPSKVQYPDY YAIIKEPIDL KTIAQRIQNG SYKSIHAMAK
260 270 280 290 300
DIDLLAKNAK TYNEPGSQVF KDANSIKKIF YMKKAEIEHH EMAKSSLRMR
310 320 330 340 350
TPSNLAAARL TGPSHSKGSL GEERNPTSKY YRNKRAVQGG RLSAITMALQ
360 370 380 390 400
YGSESEEDAA LAAARYEEGE SEAESITSFM DVSNPFYQLY DTVRSCRNNQ
410 420 430 440 450
GQLIAEPFYH LPSKKKYPDY YQQIKMPISL QQIRTKLKNQ EYETLDHLEC
460 470 480 490 500
DLNLMFENAK RYNVPNSAIY KRVLKLQQVM QAKKKELARR DDIEDGDSMI
510 520 530 540 550
SSATSDTGSA KRKSKKNIRK QRMKILFNVV LEAREPGSGR RLCDLFMVKP
560 570 580 590 600
SKKDYPDYYK IILEPMDLKI IEHNIRNDKY AGEEGMIEDM KLMFRNARHY
610 620 630 640 650
NEEGSQVYND AHILEKLLKE KRKELGPLPD DDDMASPKLK LSRKSGISPK
660 670 680 690 700
KSKYMTPMQQ KLNEVYEAVK NYTDKRGRRL SAIFLRLPSR SELPDYYLTI
710 720 730 740 750
KKPMDMEKIR SHMMANKYQD IDSMVEDFVM MFNNACTYNE PESLIYKDAL
760 770 780 790 800
VLHKVLLETR RDLEGDEDSH VPNVTLLIQE LIHNLFVSVM SHQDDEGRCY
810 820 830 840 850
SDSLAEIPAV DPNFPNKPPL TFDIIRKNVE NNRYRRLDLF QEHMFEVLER
860 870 880 890 900
ARRMNRTDSE IYEDAVELQQ FFIKIRDELC KNGEILLSPA LSYTTKHLHN
910 920 930 940 950
DVEKERKEKL PKEIEEDKLK REEEKREAEK SEDSSGAAGL SGLHRTYSQD
960 970 980 990 1000
CSFKNSMYHV GDYVYVEPAE ANLQPHIVCI ERLWEDSAGE KWLYGCWFYR
1010 1020 1030 1040 1050
PNETFHLATR KFLEKEVFKS DYYNKVPVSK ILGKCVVMFV KEYFKLCPEN
1060 1070 1080 1090 1100
FRDEDVFVCE SRYSAKTKSF KKIKLWTMPI SSVRFVPRDV PLPVVRVASV
1110 1120 1130 1140 1150
FANADKGDDE KNTDNSEDSR AEDNFNLEKE KEDVPVEMSN GEPGCHYFEQ
1160 1170 1180 1190 1200
LHYNDMWLKV GDCVFIKSHG LVRPRVGRIE KVWVRDGAAY FYGPIFIHPE
1210 1220 1230 1240 1250
ETEHEPTKMF YKKEVFLSNL EETCPMTCIL GKCAVLSFKD FLSCRPTEIP
1260 1270 1280 1290 1300
ENDILLCESR YNESDKQMKK FKGLKRFSLS AKVVDDEIYY FRKPIVPQKE
1310 1320 1330 1340 1350
PSPLLEKKIQ LLEAKFAELE GGDDDIEEMG EEDSEVIEPP SLPQLQTPLA
1360 1370 1380 1390 1400
SELDLMPYTP PQSTPKSAKG SAKKEGSKRK INMSGYILFS SEMRAVIKAQ
1410 1420 1430 1440 1450
HPDYSFGELS RLVGTEWRNL ETAKKAEYEE RAAKVAEQQE RERAAQQQQP
1460 1470 1480 1490 1500
SASPRAGTPV GALMGVVPPP TPMGMLNQQL TPVAGMMGGY PPGLPPLQGP
1510 1520 1530 1540 1550
VDGLVSMGSM QPLHPGGPPP HHLPPGVPGL PGIPPPGVMN QGVAPMVGTP
1560 1570 1580 1590 1600
APGGSPYGQQ VGVLGPPGQQ APPPYPGPHP AGPPVIQQPT TPMFVAPPPK
1610 1620 1630 1640 1650
TQRLLHSEAY LKYIEGLSAE SNSISKWDQT LAARRRDVHL SKEQESRLPS
1660 1670 1680 1689
HWLKSKGAHT TMADALWRLR DLMLRDTLNI RQAYNLENV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1362 Collapse assay details

Data source: Panorama

Official Gene Symbol
PBRM1
Peptide Modified Sequence
TYS[+80.0]QDC[+57.0]SFK
Modification Type
Phospho (ST), Carbamidomethyl (C)
Protein - Site of Modification
948, 951
Peptide - Site of Modification
3, 6
Peptide Start
946
Peptide End
954
CPTAC ID
CPTAC-1362
Peptide Molecular Mass
1,214.4315
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8-98 (2+) 7.5 5.3 5.3 8.7 10.1 13.2 11.5 11.4 14.2 15 15 15
y5 (1+) 4.8 5.1 3.8 6.1 10.4 11.6 7.8 11.6 12.2 15 15 15
y7-98 (1+) 4 3.7 4.2 6.9 9.3 10.1 8 10 10.9 15 15 15
sum 4.7 3.9 3.5 6 9.3 11 7.6 10.1 11.5 15 15 15


Additional Resources and Comments