CIC, CPTAC-1346 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CIC	KIAA0306

Sequence Length (AA)	Molecular Weight (Da)
1608	163820

Protein Name
Protein capicua homolog

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MYSAHRPLMP	ASSAASRGLG	MFVWTNVEPR	SVAVFPWHSL	VPFLAPSQPD
60	70	80	90	100
PSVQPSEAQQ	PASHPVASNQ	SKEPAESAAV	AHERPPGGTG	SADPERPPGA
110	120	130	140	150
TCPESPGPGP	PHPLGVVESG	KGPPPTTEEE	ASGPPGEPRL	DSETESDHDD
160	170	180	190	200
AFLSIMSPEI	QLPLPPGKRR	TQSLSALPKE	RDSSSEKDGR	SPNKREKDHI
210	220	230	240	250
RRPMNAFMIF	SKRHRALVHQ	RHPNQDNRTV	SKILGEWWYA	LGPKEKQKYH
260	270	280	290	300
DLAFQVKEAH	FKAHPDWKWC	NKDRKKSSSE	AKPTSLGLAG	GHKETRERSM
310	320	330	340	350
SETGTAAAPG	VSSELLSVAA	QTLLSSDTKA	PGSSSCGAER	LHTVGGPGSA
360	370	380	390	400
RPRAFSHSGV	HSLDGGEVDS	QALQELTQMV	SGPASYSGPK	PSTQYGAPGP
410	420	430	440	450
FAAPGEGGAL	AATGRPPLLP	TRASRSQRAA	SEDMTSDEER	MVICEEEGDD
460	470	480	490	500
DVIADDGFGT	TDIDLKCKER	VTDSESGDSS	GEDPEGNKGF	GRKVFSPVIR
510	520	530	540	550
SSFTHCRPPL	DPEPPGPPDP	PVAFGKGYGS	APSSSASSPA	SSSASAATSF
560	570	580	590	600
SLGSGTFKAQ	ESGQGSTAGP	LRPPPPGAGG	PATPSKATRF	LPMDPATFRR
610	620	630	640	650
KRPESVGGLE	PPGPSVIAAP	PSGGGNILQT	LVLPPNKEEQ	EGGGARVPSA
660	670	680	690	700
PAPSLAYGAP	AAPLSRPAAT	MVTNVVRPVS	STPVPIASKP	FPTSGRAEAS
710	720	730	740	750
PNDTAGARTE	MGTGSRVPGG	SPLGVSLVYS	DKKSAAATSP	APHLVAGPLL
760	770	780	790	800
GTVGKAPATV	TNLLVGTPGY	GAPAPPAVQF	IAQGAPGGGT	TAGSGAGAGS
810	820	830	840	850
GPNGPVPLGI	LQPGALGKAG	GITQVQYILP	TLPQQLQVAP	APAPAPGTKA
860	870	880	890	900
AAPSGPAPTT	SIRFTLPPGT	STNGKVLAAT	APTPGIPILQ	SVPSAPPPKA
910	920	930	940	950
QSVSPVQAPP	PGGSAQLLPG	KVLVPLAAPS	MSVRGGGAGQ	PLPLVSPPFS
960	970	980	990	1000
VPVQNGAQPP	SKIIQLTPVP	VSTPSGLVPP	LSPATLPGPT	SQPQKVLLPS
1010	1020	1030	1040	1050
STRITYVQSA	GGHALPLGTS	PASSQAGTVT	SYGPTSSVAL	GFTSLGPSGP
1060	1070	1080	1090	1100
AFVQPLLSAG	QAPLLAPGQV	GVSPVPSPQL	PPACAAPGGP	VITAFYSGSP
1110	1120	1130	1140	1150
APTSSAPLAQ	PSQAPPSLVY	TVATSTTPPA	ATILPKGPPA	PATATPAPTS
1160	1170	1180	1190	1200
PFPSATAGSM	TYSLVAPKAQ	RPSPKAPQKV	KAAIASIPVG	SFEAGASGRP
1210	1220	1230	1240	1250
GPAPRQPLEP	GPVREPTAPE	SELEGQPTPP	APPPLPETWT	PTARSSPPLP
1260	1270	1280	1290	1300
PPAEERTSAK	GPETMASKFP	SSSSDWRVPG	QGLENRGEPP	TPPSPAPAPA
1310	1320	1330	1340	1350
VAPGGSSESS	SGRAAGDTPE	RKEAAGTGKK	VKVRPPPLKK	TFDSVDNRVL
1360	1370	1380	1390	1400
SEVDFEERFA	ELPEFRPEEV	LPSPTLQSLA	TSPRAILGSY	RKKRKNSTDL
1410	1420	1430	1440	1450
DSAPEDPTSP	KRKMRRRSSC	SSEPNTPKSA	KCEGDIFTFD	RTGTEAEDVL
1460	1470	1480	1490	1500
GELEYDKVPY	SSLRRTLDQR	RALVMQLFQD	HGFFPSAQAT	AAFQARYADI
1510	1520	1530	1540	1550
FPSKVCLQLK	IREVRQKIMQ	AATPTEQPPG	AEAPLPVPPP	TGTAAAPAPT
1560	1570	1580	1590	1600
PSPAGGPDPT	SPSSDSGTAQ	AAPPLPPPPE	SGPGQPGWEG	APQPSPPPPG
1608
PSTAATGR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1346 Collapse assay details

Data source: Panorama

Official Gene Symbol: CIC
Peptide Modified Sequence: AILGS[+80.0]YR
Modification Type: Phospho (ST)
Protein - Site of Modification: 1389
Peptide - Site of Modification: 5
Peptide Start: 1385
Peptide End: 1391

CPTAC ID: CPTAC-1346
Peptide Molecular Mass: 858.4001
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel

CPTAC-1342: ACIN1. KIS[+80.0]VVSATK	CPTAC-1343: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPS[+80.0]PR	CPTAC-1344: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPSPR
CPTAC-1345: ARAF. QHEAPSNRPLNELLTPQGPS[+80.0]PR	CPTAC-1346: CIC. AILGS[+80.0]YR	CPTAC-1347: EGFR. ELVEPLT[+80.0]PSGEAPNQALLR
CPTAC-1348: GLYR1. KLS[+80.0]LSEGK	CPTAC-1349: GTF2I. GREFS[+80.0]FEAWNAK	CPTAC-1350: HMGN1. KVS[+80.0]SAEGAAKEEPK
CPTAC-1351: HSPB1. GPS[+80.0]WDPFRDWYPHSR	CPTAC-1352: MAPK1. VADPDHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1353: MAPK1. VADPDHDHTGFLT[+80.0]EYVATR
CPTAC-1354: MAPK1. VADPDHDHTGFLTEY[+80.0]VATR	CPTAC-1355: MAPK14. HTDDEMT[+80.0]GYVATR	CPTAC-1356: MAPK14. HTDDEMTGY[+80.0]VATR
CPTAC-1357: MAPK3. IADPEHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1358: MAPK3. IADPEHDHTGFLT[+80.0]EYVATR	CPTAC-1359: MAPK3. IADPEHDHTGFLTEY[+80.0]VATR
CPTAC-1360: MTOR. KLHVS[+80.0]TINLQK	CPTAC-1361: MTOR. LHVS[+80.0]TINLQK	CPTAC-1362: PBRM1. TYS[+80.0]QDC[+57.0]SFK
CPTAC-1363: PHIP. AQS[+80.0]YDIQAWKK	CPTAC-1364: PRKD2. LGTSES[+80.0]LPC[+57.0]TAEELSR	CPTAC-1365: RBM7. SFS[+80.0]SPENFQR
CPTAC-1366: SHC1. ELFDDPSY[+80.0]VNVQNLDK	CPTAC-1367: SMARCA4. EVDYSDS[+80.0]LTEKQWLK	CPTAC-1368: STMN1. ASGQAFELILS[+80.0]PR
CPTAC-1369: SYNPO2. SLS[+80.0]LPGR	CPTAC-1370: UBE2J1. QIS[+80.0]FKAEVNSSGK	CPTAC-1371: ZNF638. NYQSQADIPIRS[+80.0]PFGIVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5-98 (1+)	1.6	2	2.4	2.7	9.1	9.6	3.1	9.3	9.9	15	15	15
y5 (1+)	2.7	2	2.8	2.7	8.7	9.3	3.8	8.9	9.7	15	15	15
sum	1.6	1.8	2.4	2.8	8.8	9.4	3.2	9	9.7	15	15	15

CIC

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1346 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

CIC

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1346 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments