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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CIC KIAA0306
Sequence Length (AA) Molecular Weight (Da)
1608 163820
Protein Name
Protein capicua homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MYSAHRPLMP ASSAASRGLG MFVWTNVEPR SVAVFPWHSL VPFLAPSQPD
60 70 80 90 100
PSVQPSEAQQ PASHPVASNQ SKEPAESAAV AHERPPGGTG SADPERPPGA
110 120 130 140 150
TCPESPGPGP PHPLGVVESG KGPPPTTEEE ASGPPGEPRL DSETESDHDD
160 170 180 190 200
AFLSIMSPEI QLPLPPGKRR TQSLSALPKE RDSSSEKDGR SPNKREKDHI
210 220 230 240 250
RRPMNAFMIF SKRHRALVHQ RHPNQDNRTV SKILGEWWYA LGPKEKQKYH
260 270 280 290 300
DLAFQVKEAH FKAHPDWKWC NKDRKKSSSE AKPTSLGLAG GHKETRERSM
310 320 330 340 350
SETGTAAAPG VSSELLSVAA QTLLSSDTKA PGSSSCGAER LHTVGGPGSA
360 370 380 390 400
RPRAFSHSGV HSLDGGEVDS QALQELTQMV SGPASYSGPK PSTQYGAPGP
410 420 430 440 450
FAAPGEGGAL AATGRPPLLP TRASRSQRAA SEDMTSDEER MVICEEEGDD
460 470 480 490 500
DVIADDGFGT TDIDLKCKER VTDSESGDSS GEDPEGNKGF GRKVFSPVIR
510 520 530 540 550
SSFTHCRPPL DPEPPGPPDP PVAFGKGYGS APSSSASSPA SSSASAATSF
560 570 580 590 600
SLGSGTFKAQ ESGQGSTAGP LRPPPPGAGG PATPSKATRF LPMDPATFRR
610 620 630 640 650
KRPESVGGLE PPGPSVIAAP PSGGGNILQT LVLPPNKEEQ EGGGARVPSA
660 670 680 690 700
PAPSLAYGAP AAPLSRPAAT MVTNVVRPVS STPVPIASKP FPTSGRAEAS
710 720 730 740 750
PNDTAGARTE MGTGSRVPGG SPLGVSLVYS DKKSAAATSP APHLVAGPLL
760 770 780 790 800
GTVGKAPATV TNLLVGTPGY GAPAPPAVQF IAQGAPGGGT TAGSGAGAGS
810 820 830 840 850
GPNGPVPLGI LQPGALGKAG GITQVQYILP TLPQQLQVAP APAPAPGTKA
860 870 880 890 900
AAPSGPAPTT SIRFTLPPGT STNGKVLAAT APTPGIPILQ SVPSAPPPKA
910 920 930 940 950
QSVSPVQAPP PGGSAQLLPG KVLVPLAAPS MSVRGGGAGQ PLPLVSPPFS
960 970 980 990 1000
VPVQNGAQPP SKIIQLTPVP VSTPSGLVPP LSPATLPGPT SQPQKVLLPS
1010 1020 1030 1040 1050
STRITYVQSA GGHALPLGTS PASSQAGTVT SYGPTSSVAL GFTSLGPSGP
1060 1070 1080 1090 1100
AFVQPLLSAG QAPLLAPGQV GVSPVPSPQL PPACAAPGGP VITAFYSGSP
1110 1120 1130 1140 1150
APTSSAPLAQ PSQAPPSLVY TVATSTTPPA ATILPKGPPA PATATPAPTS
1160 1170 1180 1190 1200
PFPSATAGSM TYSLVAPKAQ RPSPKAPQKV KAAIASIPVG SFEAGASGRP
1210 1220 1230 1240 1250
GPAPRQPLEP GPVREPTAPE SELEGQPTPP APPPLPETWT PTARSSPPLP
1260 1270 1280 1290 1300
PPAEERTSAK GPETMASKFP SSSSDWRVPG QGLENRGEPP TPPSPAPAPA
1310 1320 1330 1340 1350
VAPGGSSESS SGRAAGDTPE RKEAAGTGKK VKVRPPPLKK TFDSVDNRVL
1360 1370 1380 1390 1400
SEVDFEERFA ELPEFRPEEV LPSPTLQSLA TSPRAILGSY RKKRKNSTDL
1410 1420 1430 1440 1450
DSAPEDPTSP KRKMRRRSSC SSEPNTPKSA KCEGDIFTFD RTGTEAEDVL
1460 1470 1480 1490 1500
GELEYDKVPY SSLRRTLDQR RALVMQLFQD HGFFPSAQAT AAFQARYADI
1510 1520 1530 1540 1550
FPSKVCLQLK IREVRQKIMQ AATPTEQPPG AEAPLPVPPP TGTAAAPAPT
1560 1570 1580 1590 1600
PSPAGGPDPT SPSSDSGTAQ AAPPLPPPPE SGPGQPGWEG APQPSPPPPG
1608
PSTAATGR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1346 Collapse assay details

Data source: Panorama

Official Gene Symbol
CIC
Peptide Modified Sequence
AILGS[+80.0]YR
Modification Type
Phospho (ST)
Protein - Site of Modification
1389
Peptide - Site of Modification
5
Peptide Start
1385
Peptide End
1391
CPTAC ID
CPTAC-1346
Peptide Molecular Mass
858.4001
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5-98 (1+) 1.6 2 2.4 2.7 9.1 9.6 3.1 9.3 9.9 15 15 15
y5 (1+) 2.7 2 2.8 2.7 8.7 9.3 3.8 8.9 9.7 15 15 15
sum 1.6 1.8 2.4 2.8 8.8 9.4 3.2 9 9.7 15 15 15

Additional Resources and Comments