PRKD2, CPTAC-1364 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKD2	PKD2

Sequence Length (AA)	Molecular Weight (Da)
878	96750

Protein Name
Serine/threonine-protein kinase D2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATAPSYPAG	LPGSPGPGSP	PPPGGLELQS	PPPLLPQIPA	PGSGVSFHIQ
60	70	80	90	100
IGLTREFVLL	PAASELAHVK	QLACSIVDQK	FPECGFYGLY	DKILLFKHDP
110	120	130	140	150
TSANLLQLVR	SSGDIQEGDL	VEVVLSASAT	FEDFQIRPHA	LTVHSYRAPA
160	170	180	190	200
FCDHCGEMLF	GLVRQGLKCD	GCGLNYHKRC	AFSIPNNCSG	ARKRRLSSTS
210	220	230	240	250
LASGHSVRLG	TSESLPCTAE	ELSRSTTELL	PRRPPSSSSS	SSASSYTGRP
260	270	280	290	300
IELDKMLLSK	VKVPHTFLIH	SYTRPTVCQA	CKKLLKGLFR	QGLQCKDCKF
310	320	330	340	350
NCHKRCATRV	PNDCLGEALI	NGDVPMEEAT	DFSEADKSAL	MDESEDSGVI
360	370	380	390	400
PGSHSENALH	ASEEEEGEGG	KAQSSLGYIP	LMRVVQSVRH	TTRKSSTTLR
410	420	430	440	450
EGWVVHYSNK	DTLRKRHYWR	LDCKCITLFQ	NNTTNRYYKE	IPLSEILTVE
460	470	480	490	500
SAQNFSLVPP	GTNPHCFEIV	TANATYFVGE	MPGGTPGGPS	GQGAEAARGW
510	520	530	540	550
ETAIRQALMP	VILQDAPSAP	GHAPHRQASL	SISVSNSQIQ	ENVDIATVYQ
560	570	580	590	600
IFPDEVLGSG	QFGVVYGGKH	RKTGRDVAVK	VIDKLRFPTK	QESQLRNEVA
610	620	630	640	650
ILQSLRHPGI	VNLECMFETP	EKVFVVMEKL	HGDMLEMILS	SEKGRLPERL
660	670	680	690	700
TKFLITQILV	ALRHLHFKNI	VHCDLKPENV	LLASADPFPQ	VKLCDFGFAR
710	720	730	740	750
IIGEKSFRRS	VVGTPAYLAP	EVLLNQGYNR	SLDMWSVGVI	MYVSLSGTFP
760	770	780	790	800
FNEDEDINDQ	IQNAAFMYPA	SPWSHISAGA	IDLINNLLQV	KMRKRYSVDK
810	820	830	840	850
SLSHPWLQEY	QTWLDLRELE	GKMGERYITH	ESDDVRWEQF	AAEHPLPGSG
860	870	878
LPTDRDLGGA	CPPQDHDMQG	LAERISVL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1364 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKD2
Peptide Modified Sequence: LGTSES[+80.0]LPC[+57.0]TAEELSR
Modification Type: Phospho (ST), Carbamidomethyl (C)
Protein - Site of Modification: 214, 217
Peptide - Site of Modification: 6, 9
Peptide Start: 209
Peptide End: 224

CPTAC ID: CPTAC-1364
Peptide Molecular Mass: 1,828.7914
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel

CPTAC-1342: ACIN1. KIS[+80.0]VVSATK	CPTAC-1343: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPS[+80.0]PR	CPTAC-1344: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPSPR
CPTAC-1345: ARAF. QHEAPSNRPLNELLTPQGPS[+80.0]PR	CPTAC-1346: CIC. AILGS[+80.0]YR	CPTAC-1347: EGFR. ELVEPLT[+80.0]PSGEAPNQALLR
CPTAC-1348: GLYR1. KLS[+80.0]LSEGK	CPTAC-1349: GTF2I. GREFS[+80.0]FEAWNAK	CPTAC-1350: HMGN1. KVS[+80.0]SAEGAAKEEPK
CPTAC-1351: HSPB1. GPS[+80.0]WDPFRDWYPHSR	CPTAC-1352: MAPK1. VADPDHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1353: MAPK1. VADPDHDHTGFLT[+80.0]EYVATR
CPTAC-1354: MAPK1. VADPDHDHTGFLTEY[+80.0]VATR	CPTAC-1355: MAPK14. HTDDEMT[+80.0]GYVATR	CPTAC-1356: MAPK14. HTDDEMTGY[+80.0]VATR
CPTAC-1357: MAPK3. IADPEHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1358: MAPK3. IADPEHDHTGFLT[+80.0]EYVATR	CPTAC-1359: MAPK3. IADPEHDHTGFLTEY[+80.0]VATR
CPTAC-1360: MTOR. KLHVS[+80.0]TINLQK	CPTAC-1361: MTOR. LHVS[+80.0]TINLQK	CPTAC-1362: PBRM1. TYS[+80.0]QDC[+57.0]SFK
CPTAC-1363: PHIP. AQS[+80.0]YDIQAWKK	CPTAC-1364: PRKD2. LGTSES[+80.0]LPC[+57.0]TAEELSR	CPTAC-1365: RBM7. SFS[+80.0]SPENFQR
CPTAC-1366: SHC1. ELFDDPSY[+80.0]VNVQNLDK	CPTAC-1367: SMARCA4. EVDYSDS[+80.0]LTEKQWLK	CPTAC-1368: STMN1. ASGQAFELILS[+80.0]PR
CPTAC-1369: SYNPO2. SLS[+80.0]LPGR	CPTAC-1370: UBE2J1. QIS[+80.0]FKAEVNSSGK	CPTAC-1371: ZNF638. NYQSQADIPIRS[+80.0]PFGIVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7-98 (1+)	3.6	4.2	3.7	4.3	5	7	5.6	6.5	7.9	15	15	15
y7 (1+)	5.8	5.8	4.4	8.6	6.9	6.7	10.4	9	8	14	15	15
y9 (1+)	2	3.7	1.1	2.6	6.3	5.8	3.3	7.3	5.9	15	15	15
sum	2.1	3.5	1.1	2.9	5.9	5.8	3.6	6.9	5.9	15	15	15

PRKD2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1364 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

PRKD2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1364 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments