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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SYNPO2 N/A
Sequence Length (AA) Molecular Weight (Da)
1230 117514
Protein Name
Synaptopodin-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVTQIRNQSK ASGSGLCEGD EVVSINGNPC ADLTYPEVIK LMESITDSLQ
60 70 80 90 100
MLIKRPSSGI SEALISENEN KNLEHLTHGG YVESTTLQIR PATKTQCTEF
110 120 130 140 150
FLAPVKTEVP LAENQRSGPD CAGSLKEETG PSYQRAPQMP DSQRGRVAEE
160 170 180 190 200
LILREKVEAV QPGPVVELQL SLSQERHKGA SGPLVALPGA EKSKSPDPDP
210 220 230 240 250
NLSHDRIVHI NSIPTNEKAD PFLRSSKIIQ ISSGRELRVI QESEAGDAGL
260 270 280 290 300
PRVEVILDCS DRQKTEGCRL QAGKECVDSP VEGGQSEAPP SLVSFAVSSE
310 320 330 340 350
GTEQGEDPRS EKDHSRPHKH RARHARLRRS ESLSEKQVKE AKSKCKSIAL
360 370 380 390 400
LLTDAPNPNS KGVLMFKKRR RRARKYTLVS YGTGELEREA DEEEEGDKED
410 420 430 440 450
TCEVAFLGAS ESEVDEELLS DVDDNTQVVN FDWDSGLVDI EKKLNRGDKM
460 470 480 490 500
EMLPDTTGKG ALMFAKRRER MDQITAQKEE DKVGGTPSRE QDAAQTDGLR
510 520 530 540 550
TTTSYQRKEE ESVRTQSSVS KSYIEVSHGL GHVPQQNGFS GTSETANIQR
560 570 580 590 600
MVPMNRTAKP FPGSVNQPAT PFSPTRNMTS PIADFPAPPP YSAVTPPPDA
610 620 630 640 650
FSRGVSSPIA GPAQPPPWPQ PAPWSQPAFY DSSERIASRD ERISVPAKRT
660 670 680 690 700
GILQEAKRRS TTKPMFTFKE PKVSPNPELL SLLQNSEGKR GTGAGGDSGP
710 720 730 740 750
EEDYLSLGAE ACNFMQSSSA KQKTPPPVAP KPAVKSSSSQ PVTPVSPVWS
760 770 780 790 800
PGVAPTQPPA FPTSNPSKGT VVSSIKIAQP SYPPARPAST LNVAGPFKGP
810 820 830 840 850
QAAVASQNYT PKPTVSTPTV NAVQPGAVGP SNELPGMSGR GAQLFAKRQS
860 870 880 890 900
RMEKYVVDSD TVQAHAARAQ SPTPSLPASW KYSSNVRAPP PVAYNPIHSP
910 920 930 940 950
SYPLAALKSQ PSAAQPSKMG KKKGKKPLNA LDVMKHQPYQ LNASLFTFQP
960 970 980 990 1000
PDAKDGLPQK SSVKVNSALA MKQALPPRPV NAASPTNVQA SSVYSVPAYT
1010 1020 1030 1040 1050
SPPSFFAEAS SPVSASPVPV GIPTSPKQES ASSSYFVAPR PKFSAKKSGV
1060 1070 1080 1090 1100
TIQESGRSLS LPGRSVPPPI STSPWVYQPT YSYSSKPTDG LEKANKRPTP
1110 1120 1130 1140 1150
WEAAAKSPLG LVDDAFQPRN IQESIVANVV SAARRKVLPG PPEDWNERLS
1160 1170 1180 1190 1200
YIPQTQKAYM GSCGRQEYNV TANNNMSTTS QYGSQLPYAY YRQASRNDSA
1210 1220 1230
IMSMETRSDY CLPVADYNYN PHPRGWRRQT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1369 Collapse assay details

Data source: Panorama

Official Gene Symbol
SYNPO2
Peptide Modified Sequence
SLS[+80.0]LPGR
Modification Type
Phospho (ST)
Protein - Site of Modification
1060
Peptide - Site of Modification
3
Peptide Start
1058
Peptide End
1064
CPTAC ID
CPTAC-1369
Peptide Molecular Mass
808.3844
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 3.3 2.2 1.5 13.8 20.1 8 14.2 20.2 8.1 15 15 15
y5-98 (1+) 1 1.3 1.7 3.5 8.5 8.4 3.6 8.6 8.6 15 15 15
y5 (1+) 1.6 2.9 1.6 4 8.3 8 4.3 8.8 8.2 15 15 15
sum 0.9 1.3 1.6 3.5 8.3 7.6 3.6 8.4 7.8 15 15 15


Additional Resources and Comments