SYNPO2, CPTAC-1369 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SYNPO2	N/A

Sequence Length (AA)	Molecular Weight (Da)
1230	117514

Protein Name
Synaptopodin-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVTQIRNQSK	ASGSGLCEGD	EVVSINGNPC	ADLTYPEVIK	LMESITDSLQ
60	70	80	90	100
MLIKRPSSGI	SEALISENEN	KNLEHLTHGG	YVESTTLQIR	PATKTQCTEF
110	120	130	140	150
FLAPVKTEVP	LAENQRSGPD	CAGSLKEETG	PSYQRAPQMP	DSQRGRVAEE
160	170	180	190	200
LILREKVEAV	QPGPVVELQL	SLSQERHKGA	SGPLVALPGA	EKSKSPDPDP
210	220	230	240	250
NLSHDRIVHI	NSIPTNEKAD	PFLRSSKIIQ	ISSGRELRVI	QESEAGDAGL
260	270	280	290	300
PRVEVILDCS	DRQKTEGCRL	QAGKECVDSP	VEGGQSEAPP	SLVSFAVSSE
310	320	330	340	350
GTEQGEDPRS	EKDHSRPHKH	RARHARLRRS	ESLSEKQVKE	AKSKCKSIAL
360	370	380	390	400
LLTDAPNPNS	KGVLMFKKRR	RRARKYTLVS	YGTGELEREA	DEEEEGDKED
410	420	430	440	450
TCEVAFLGAS	ESEVDEELLS	DVDDNTQVVN	FDWDSGLVDI	EKKLNRGDKM
460	470	480	490	500
EMLPDTTGKG	ALMFAKRRER	MDQITAQKEE	DKVGGTPSRE	QDAAQTDGLR
510	520	530	540	550
TTTSYQRKEE	ESVRTQSSVS	KSYIEVSHGL	GHVPQQNGFS	GTSETANIQR
560	570	580	590	600
MVPMNRTAKP	FPGSVNQPAT	PFSPTRNMTS	PIADFPAPPP	YSAVTPPPDA
610	620	630	640	650
FSRGVSSPIA	GPAQPPPWPQ	PAPWSQPAFY	DSSERIASRD	ERISVPAKRT
660	670	680	690	700
GILQEAKRRS	TTKPMFTFKE	PKVSPNPELL	SLLQNSEGKR	GTGAGGDSGP
710	720	730	740	750
EEDYLSLGAE	ACNFMQSSSA	KQKTPPPVAP	KPAVKSSSSQ	PVTPVSPVWS
760	770	780	790	800
PGVAPTQPPA	FPTSNPSKGT	VVSSIKIAQP	SYPPARPAST	LNVAGPFKGP
810	820	830	840	850
QAAVASQNYT	PKPTVSTPTV	NAVQPGAVGP	SNELPGMSGR	GAQLFAKRQS
860	870	880	890	900
RMEKYVVDSD	TVQAHAARAQ	SPTPSLPASW	KYSSNVRAPP	PVAYNPIHSP
910	920	930	940	950
SYPLAALKSQ	PSAAQPSKMG	KKKGKKPLNA	LDVMKHQPYQ	LNASLFTFQP
960	970	980	990	1000
PDAKDGLPQK	SSVKVNSALA	MKQALPPRPV	NAASPTNVQA	SSVYSVPAYT
1010	1020	1030	1040	1050
SPPSFFAEAS	SPVSASPVPV	GIPTSPKQES	ASSSYFVAPR	PKFSAKKSGV
1060	1070	1080	1090	1100
TIQESGRSLS	LPGRSVPPPI	STSPWVYQPT	YSYSSKPTDG	LEKANKRPTP
1110	1120	1130	1140	1150
WEAAAKSPLG	LVDDAFQPRN	IQESIVANVV	SAARRKVLPG	PPEDWNERLS
1160	1170	1180	1190	1200
YIPQTQKAYM	GSCGRQEYNV	TANNNMSTTS	QYGSQLPYAY	YRQASRNDSA
1210	1220	1230
IMSMETRSDY	CLPVADYNYN	PHPRGWRRQT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1369 Collapse assay details

Data source: Panorama

Official Gene Symbol: SYNPO2
Peptide Modified Sequence: SLS[+80.0]LPGR
Modification Type: Phospho (ST)
Protein - Site of Modification: 1060
Peptide - Site of Modification: 3
Peptide Start: 1058
Peptide End: 1064

CPTAC ID: CPTAC-1369
Peptide Molecular Mass: 808.3844
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel

CPTAC-1342: ACIN1. KIS[+80.0]VVSATK	CPTAC-1343: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPS[+80.0]PR	CPTAC-1344: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPSPR
CPTAC-1345: ARAF. QHEAPSNRPLNELLTPQGPS[+80.0]PR	CPTAC-1346: CIC. AILGS[+80.0]YR	CPTAC-1347: EGFR. ELVEPLT[+80.0]PSGEAPNQALLR
CPTAC-1348: GLYR1. KLS[+80.0]LSEGK	CPTAC-1349: GTF2I. GREFS[+80.0]FEAWNAK	CPTAC-1350: HMGN1. KVS[+80.0]SAEGAAKEEPK
CPTAC-1351: HSPB1. GPS[+80.0]WDPFRDWYPHSR	CPTAC-1352: MAPK1. VADPDHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1353: MAPK1. VADPDHDHTGFLT[+80.0]EYVATR
CPTAC-1354: MAPK1. VADPDHDHTGFLTEY[+80.0]VATR	CPTAC-1355: MAPK14. HTDDEMT[+80.0]GYVATR	CPTAC-1356: MAPK14. HTDDEMTGY[+80.0]VATR
CPTAC-1357: MAPK3. IADPEHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1358: MAPK3. IADPEHDHTGFLT[+80.0]EYVATR	CPTAC-1359: MAPK3. IADPEHDHTGFLTEY[+80.0]VATR
CPTAC-1360: MTOR. KLHVS[+80.0]TINLQK	CPTAC-1361: MTOR. LHVS[+80.0]TINLQK	CPTAC-1362: PBRM1. TYS[+80.0]QDC[+57.0]SFK
CPTAC-1363: PHIP. AQS[+80.0]YDIQAWKK	CPTAC-1364: PRKD2. LGTSES[+80.0]LPC[+57.0]TAEELSR	CPTAC-1365: RBM7. SFS[+80.0]SPENFQR
CPTAC-1366: SHC1. ELFDDPSY[+80.0]VNVQNLDK	CPTAC-1367: SMARCA4. EVDYSDS[+80.0]LTEKQWLK	CPTAC-1368: STMN1. ASGQAFELILS[+80.0]PR
CPTAC-1369: SYNPO2. SLS[+80.0]LPGR	CPTAC-1370: UBE2J1. QIS[+80.0]FKAEVNSSGK	CPTAC-1371: ZNF638. NYQSQADIPIRS[+80.0]PFGIVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	3.3	2.2	1.5	13.8	20.1	8	14.2	20.2	8.1	15	15	15
y5-98 (1+)	1	1.3	1.7	3.5	8.5	8.4	3.6	8.6	8.6	15	15	15
y5 (1+)	1.6	2.9	1.6	4	8.3	8	4.3	8.8	8.2	15	15	15
sum	0.9	1.3	1.6	3.5	8.3	7.6	3.6	8.4	7.8	15	15	15

SYNPO2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1369 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SYNPO2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1369 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments