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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ZNF638 NP220, ZFML
Sequence Length (AA) Molecular Weight (Da)
1978 220625
Protein Name
Zinc finger protein 638
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSRPRFNPRG DFPLQRPRAP NPSGMRPPGP FMRPGSMGLP RFYPAGRARG
60 70 80 90 100
IPHRFAGHES YQNMGPQRMN VQVTQHRTDP RLTKEKLDFH EAQQKKGKPH
110 120 130 140 150
GSRWDDEPHI SASVAVKQSS VTQVTEQSPK VQSRYTKESA SSILASFGLS
160 170 180 190 200
NEDLEELSRY PDEQLTPENM PLILRDIRMR KMGRRLPNLP SQSRNKETLG
210 220 230 240 250
SEAVSSNVID YGHASKYGYT EDPLEVRIYD PEIPTDEVEN EFQSQQNISA
260 270 280 290 300
SVPNPNVICN SMFPVEDVFR QMDFPGESSN NRSFFSVESG TKMSGLHISG
310 320 330 340 350
GQSVLEPIKS VNQSINQTVS QTMSQSLIPP SMNQQPFSSE LISSVSQQER
360 370 380 390 400
IPHEPVINSS NVHVGSRGSK KNYQSQADIP IRSPFGIVKA SWLPKFSHAD
410 420 430 440 450
AQKMKRLPTP SMMNDYYAAS PRIFPHLCSL CNVECSHLKD WIQHQNTSTH
460 470 480 490 500
IESCRQLRQQ YPDWNPEILP SRRNEGNRKE NETPRRRSHS PSPRRSRRSS
510 520 530 540 550
SSHRFRRSRS PMHYMYRPRS RSPRICHRFI SRYRSRSRSR SPYRIRNPFR
560 570 580 590 600
GSPKCFRSVS PERMSRRSVR SSDRKKALED VVQRSGHGTE FNKQKHLEAA
610 620 630 640 650
DKGHSPAQKP KTSSGTKPSV KPTSATKSDS NLGGHSIRCK SKNLEDDTLS
660 670 680 690 700
ECKQVSDKAV SLQRKLRKEQ SLHYGSVLLI TELPEDGCTE EDVRKLFQPF
710 720 730 740 750
GKVNDVLIVP YRKEAYLEME FKEAITAIMK YIETTPLTIK GKSVKICVPG
760 770 780 790 800
KKKAQNKEVK KKTLESKKVS ASTLKRDADA SKAVEIVTST SAAKTGQAKA
810 820 830 840 850
SVAKVNKSTG KSASSVKSVV TVAVKGNKAS IKTAKSGGKK SLEAKKTGNV
860 870 880 890 900
KNKDSNKPVT IPENSEIKTS IEVKATENCA KEAISDAALE ATENEPLNKE
910 920 930 940 950
TEEMCVMLVS NLPNKGYSVE EVYDLAKPFG GLKDILILSS HKKAYIEINR
960 970 980 990 1000
KAAESMVKFY TCFPVLMDGN QLSISMAPEN MNIKDEEAIF ITLVKENDPE
1010 1020 1030 1040 1050
ANIDTIYDRF VHLDNLPEDG LQCVLCVGLQ FGKVDHHVFI SNRNKAILQL
1060 1070 1080 1090 1100
DSPESAQSMY SFLKQNPQNI GDHMLTCSLS PKIDLPEVQI EHDPELEKES
1110 1120 1130 1140 1150
PGLKNSPIDE SEVQTATDSP SVKPNELEEE STPSIQTETL VQQEEPCEEE
1160 1170 1180 1190 1200
AEKATCDSDF AVETLELETQ GEEVKEEIPL VASASVSIEQ FTENAEECAL
1210 1220 1230 1240 1250
NQQMFNSDLE KKGAEIINPK TALLPSDSVF AEERNLKGIL EESPSEAEDF
1260 1270 1280 1290 1300
ISGITQTMVE AVAEVEKNET VSEILPSTCI VTLVPGIPTG DEKTVDKKNI
1310 1320 1330 1340 1350
SEKKGNMDEK EEKEFNTKET RMDLQIGTEK AEKNEGRMDA EKVEKMAAMK
1360 1370 1380 1390 1400
EKPAENTLFK AYPNKGVGQA NKPDETSKTS ILAVSDVSSS KPSIKAVIVS
1410 1420 1430 1440 1450
SPKAKATVSK TENQKSFPKS VPRDQINAEK KLSAKEFGLL KPTSARSGLA
1460 1470 1480 1490 1500
ESSSKFKPTQ SSLTRGGSGR ISALQGKLSK LDYRDITKQS QETEARPSIM
1510 1520 1530 1540 1550
KRDDSNNKTL AEQNTKNPKS TTGRSSKSKE EPLFPFNLDE FVTVDEVIEE
1560 1570 1580 1590 1600
VNPSQAKQNP LKGKRKETLK NVPFSELNLK KKKGKTSTPR GVEGELSFVT
1610 1620 1630 1640 1650
LDEIGEEEDA AAHLAQALVT VDEVIDEEEL NMEEMVKNSN SLFTLDELID
1660 1670 1680 1690 1700
QDDCISHSEP KDVTVLSVAE EQDLLKQERL VTVDEIGEVE ELPLNESADI
1710 1720 1730 1740 1750
TFATLNTKGN EGDTVRDSIG FISSQVPEDP STLVTVDEIQ DDSSDLHLVT
1760 1770 1780 1790 1800
LDEVTEEDED SLADFNNLKE ELNFVTVDEV GEEEDGDNDL KVELAQSKND
1810 1820 1830 1840 1850
HPTDKKGNRK KRAVDTKKTK LESLSQVGPV NENVMEEDLK TMIERHLTAK
1860 1870 1880 1890 1900
TPTKRVRIGK TLPSEKAVVT EPAKGEEAFQ MSEVDEESGL KDSEPERKRK
1910 1920 1930 1940 1950
KTEDSSSGKS VASDVPEELD FLVPKAGFFC PICSLFYSGE KAMTNHCKST
1960 1970 1978
RHKQNTEKFM AKQRKEKEQN EAEERSSR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1371 Collapse assay details

Data source: Panorama

Official Gene Symbol
ZNF638
Peptide Modified Sequence
NYQSQADIPIRS[+80.0]PFGIVK
Modification Type
Phospho (ST)
Protein - Site of Modification
383
Peptide - Site of Modification
12
Peptide Start
372
Peptide End
389
CPTAC ID
CPTAC-1371
Peptide Molecular Mass
2,112.0405
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 4.7 6.7 6.9 7.4 11.5 8.3 8.8 13.3 10.8 15 15 15
y11 (2+) 6.5 7 7.6 9.9 11.5 8.7 11.8 13.5 11.6 15 15 15
y13 (2+) 3.7 6.8 6.8 7 9.1 10 7.9 11.4 12.1 15 15 15
sum 3.9 5.7 6.7 6 10.2 8.1 7.2 11.7 10.5 15 15 15

Additional Resources and Comments