GTF2I, CPTAC-1349 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GTF2I	BAP135, WBSCR6

Sequence Length (AA)	Molecular Weight (Da)
998	112416

Protein Name
General transcription factor II-I

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAQVAMSTLP	VEDEESSESR	MVVTFLMSAL	ESMCKELAKS	KAEVACIAVY
60	70	80	90	100
ETDVFVVGTE	RGRAFVNTRK	DFQKDFVKYC	VEEEEKAAEM	HKMKSTTQAN
110	120	130	140	150
RMSVDAVEIE	TLRKTVEDYF	CFCYGKALGK	STVVPVPYEK	MLRDQSAVVV
160	170	180	190	200
QGLPEGVAFK	HPENYDLATL	KWILENKAGI	SFIIKRPFLE	PKKHVGGRVM
210	220	230	240	250
VTDADRSILS	PGGSCGPIKV	KTEPTEDSGI	SLEMAAVTVK	EESEDPDYYQ
260	270	280	290	300
YNIQAGPSET	DDVDEKQPLS	KPLQGSHHSS	EGNEGTEMEV	PAEDSTQHVP
310	320	330	340	350
SETSEDPEVE	VTIEDDDYSP	PSKRPKANEL	PQPPVPEPAN	AGKRKVREFN
360	370	380	390	400
FEKWNARITD	LRKQVEELFE	RKYAQAIKAK	GPVTIPYPLF	QSHVEDLYVE
410	420	430	440	450
GLPEGIPFRR	PSTYGIPRLE	RILLAKERIR	FVIKKHELLN	STREDLQLDK
460	470	480	490	500
PASGVKEEWY	ARITKLRKMV	DQLFCKKFAE	ALGSTEAKAV	PYQKFEAHPN
510	520	530	540	550
DLYVEGLPEN	IPFRSPSWYG	IPRLEKIIQV	GNRIKFVIKR	PELLTHSTTE
560	570	580	590	600
VTQPRTNTPV	KEDWNVRITK	LRKQVEEIFN	LKFAQALGLT	EAVKVPYPVF
610	620	630	640	650
ESNPEFLYVE	GLPEGIPFRS	PTWFGIPRLE	RIVRGSNKIK	FVVKKPELVI
660	670	680	690	700
SYLPPGMASK	INTKALQSPK	RPRSPGSNSK	VPEIEVTVEG	PNNNNPQTSA
710	720	730	740	750
VRTPTQTNGS	NVPFKPRGRE	FSFEAWNAKI	TDLKQKVENL	FNEKCGEALG
760	770	780	790	800
LKQAVKVPFA	LFESFPEDFY	VEGLPEGVPF	RRPSTFGIPR	LEKILRNKAK
810	820	830	840	850
IKFIIKKPEM	FETAIKESTS	SKSPPRKINS	SPNVNTTASG	VEDLNIIQVT
860	870	880	890	900
IPDDDNERLS	KVEKARQLRE	QVNDLFSRKF	GEAIGMGFPV	KVPYRKITIN
910	920	930	940	950
PGCVVVDGMP	PGVSFKAPSY	LEISSMRRIL	DSAEFIKFTV	IRPFPGLVIN
960	970	980	990	998
NQLVDQSESE	GPVIQESAEP	SQLEVPATEE	IKETDGSSQI	KQEPDPTW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1349 Collapse assay details

Data source: Panorama

Official Gene Symbol: GTF2I
Peptide Modified Sequence: GREFS[+80.0]FEAWNAK
Modification Type: Phospho (ST)
Protein - Site of Modification: 722
Peptide - Site of Modification: 5
Peptide Start: 718
Peptide End: 729

CPTAC ID: CPTAC-1349
Peptide Molecular Mass: 1,520.6449
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel

CPTAC-1342: ACIN1. KIS[+80.0]VVSATK	CPTAC-1343: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPS[+80.0]PR	CPTAC-1344: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPSPR
CPTAC-1345: ARAF. QHEAPSNRPLNELLTPQGPS[+80.0]PR	CPTAC-1346: CIC. AILGS[+80.0]YR	CPTAC-1347: EGFR. ELVEPLT[+80.0]PSGEAPNQALLR
CPTAC-1348: GLYR1. KLS[+80.0]LSEGK	CPTAC-1349: GTF2I. GREFS[+80.0]FEAWNAK	CPTAC-1350: HMGN1. KVS[+80.0]SAEGAAKEEPK
CPTAC-1351: HSPB1. GPS[+80.0]WDPFRDWYPHSR	CPTAC-1352: MAPK1. VADPDHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1353: MAPK1. VADPDHDHTGFLT[+80.0]EYVATR
CPTAC-1354: MAPK1. VADPDHDHTGFLTEY[+80.0]VATR	CPTAC-1355: MAPK14. HTDDEMT[+80.0]GYVATR	CPTAC-1356: MAPK14. HTDDEMTGY[+80.0]VATR
CPTAC-1357: MAPK3. IADPEHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1358: MAPK3. IADPEHDHTGFLT[+80.0]EYVATR	CPTAC-1359: MAPK3. IADPEHDHTGFLTEY[+80.0]VATR
CPTAC-1360: MTOR. KLHVS[+80.0]TINLQK	CPTAC-1361: MTOR. LHVS[+80.0]TINLQK	CPTAC-1362: PBRM1. TYS[+80.0]QDC[+57.0]SFK
CPTAC-1363: PHIP. AQS[+80.0]YDIQAWKK	CPTAC-1364: PRKD2. LGTSES[+80.0]LPC[+57.0]TAEELSR	CPTAC-1365: RBM7. SFS[+80.0]SPENFQR
CPTAC-1366: SHC1. ELFDDPSY[+80.0]VNVQNLDK	CPTAC-1367: SMARCA4. EVDYSDS[+80.0]LTEKQWLK	CPTAC-1368: STMN1. ASGQAFELILS[+80.0]PR
CPTAC-1369: SYNPO2. SLS[+80.0]LPGR	CPTAC-1370: UBE2J1. QIS[+80.0]FKAEVNSSGK	CPTAC-1371: ZNF638. NYQSQADIPIRS[+80.0]PFGIVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7-98 (2+)	8	7.3	6	11	10.4	10.8	13.6	12.7	12.4	15	15	15
y4 (1+)	4.6	7	5.2	6.8	13.2	13.3	8.2	14.9	14.3	15	15	15
y5 (1+)	5.6	5.9	4.2	7.8	12.5	9.1	9.6	13.8	10	15	15	15
sum	5.4	4.2	3.5	7.4	11	10.3	9.2	11.8	10.9	15	15	15

GTF2I

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1349 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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GTF2I

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1349 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments