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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PHIP WDR11
Sequence Length (AA) Molecular Weight (Da)
1821 206689
Protein Name
PH-interacting protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSCERKGLSE LRSELYFLIA RFLEDGPCQQ AAQVLIREVA EKELLPRRTD
60 70 80 90 100
WTGKEHPRTY QNLVKYYRHL APDHLLQICH RLGPLLEQEI PQSVPGVQTL
110 120 130 140 150
LGAGRQSLLR TNKSCKHVVW KGSALAALHC GRPPESPVNY GSPPSIADTL
160 170 180 190 200
FSRKLNGKYR LERLVPTAVY QHMKMHKRIL GHLSSVYCVT FDRTGRRIFT
210 220 230 240 250
GSDDCLVKIW ATDDGRLLAT LRGHAAEISD MAVNYENTMI AAGSCDKMIR
260 270 280 290 300
VWCLRTCAPL AVLQGHSASI TSLQFSPLCS GSKRYLSSTG ADGTICFWLW
310 320 330 340 350
DAGTLKINPR PAKFTERPRP GVQMICSSFS AGGMFLATGS TDHIIRVYFF
360 370 380 390 400
GSGQPEKISE LEFHTDKVDS IQFSNTSNRF VSGSRDGTAR IWQFKRREWK
410 420 430 440 450
SILLDMATRP AGQNLQGIED KITKMKVTMV AWDRHDNTVI TAVNNMTLKV
460 470 480 490 500
WNSYTGQLIH VLMGHEDEVF VLEPHPFDPR VLFSAGHDGN VIVWDLARGV
510 520 530 540 550
KIRSYFNMIE GQGHGAVFDC KCSPDGQHFA CTDSHGHLLI FGFGSSSKYD
560 570 580 590 600
KIADQMFFHS DYRPLIRDAN NFVLDEQTQQ APHLMPPPFL VDVDGNPHPS
610 620 630 640 650
RYQRLVPGRE NCREEQLIPQ MGVTSSGLNQ VLSQQANQEI SPLDSMIQRL
660 670 680 690 700
QQEQDLRRSG EAVISNTSRL SRGSISSTSE VHSPPNVGLR RSGQIEGVRQ
710 720 730 740 750
MHSNAPRSEI ATERDLVAWS RRVVVPELSA GVASRQEEWR TAKGEEEIKT
760 770 780 790 800
YRSEEKRKHL TVPKENKIPT VSKNHAHEHF LDLGESKKQQ TNQHNYRTRS
810 820 830 840 850
ALEETPRPSE EIENGSSSSD EGEVVAVSGG TSEEEERAWH SDGSSSDYSS
860 870 880 890 900
DYSDWTADAG INLQPPKKVP KNKTKKAESS SDEEEESEKQ KQKQIKKEKK
910 920 930 940 950
KVNEEKDGPI SPKKKKPKER KQKRLAVGEL TENGLTLEEW LPSTWITDTI
960 970 980 990 1000
PRRCPFVPQM GDEVYYFRQG HEAYVEMARK NKIYSINPKK QPWHKMELRE
1010 1020 1030 1040 1050
QELMKIVGIK YEVGLPTLCC LKLAFLDPDT GKLTGGSFTM KYHDMPDVID
1060 1070 1080 1090 1100
FLVLRQQFDD AKYRRWNIGD RFRSVIDDAW WFGTIESQEP LQLEYPDSLF
1110 1120 1130 1140 1150
QCYNVCWDNG DTEKMSPWDM ELIPNNAVFP EELGTSVPLT DGECRSLIYK
1160 1170 1180 1190 1200
PLDGEWGTNP RDEECERIVA GINQLMTLDI ASAFVAPVDL QAYPMYCTVV
1210 1220 1230 1240 1250
AYPTDLSTIK QRLENRFYRR VSSLMWEVRY IEHNTRTFNE PGSPIVKSAK
1260 1270 1280 1290 1300
FVTDLLLHFI KDQTCYNIIP LYNSMKKKVL SDSEDEEKDA DVPGTSTRKR
1310 1320 1330 1340 1350
KDHQPRRRLR NRAQSYDIQA WKKQCEELLN LIFQCEDSEP FRQPVDLLEY
1360 1370 1380 1390 1400
PDYRDIIDTP MDFATVRETL EAGNYESPME LCKDVRLIFS NSKAYTPSKR
1410 1420 1430 1440 1450
SRIYSMSLRL SAFFEEHISS VLSDYKSALR FHKRNTITKR RKKRNRSSSV
1460 1470 1480 1490 1500
SSSAASSPER KKRILKPQLK SESSTSAFST PTRSIPPRHN AAQINGKTES
1510 1520 1530 1540 1550
SSVVRTRSNR VVVDPVVTEQ PSTSSAAKTF ITKANASAIP GKTILENSVK
1560 1570 1580 1590 1600
HSKALNTLSS PGQSSFSHGT RNNSAKENME KEKPVKRKMK SSVLPKASTL
1610 1620 1630 1640 1650
SKSSAVIEQG DCKNNALVPG TIQVNGHGGQ PSKLVKRGPG RKPKVEVNTN
1660 1670 1680 1690 1700
SGEIIHKKRG RKPKKLQYAK PEDLEQNNVH PIRDEVLPSS TCNFLSETNN
1710 1720 1730 1740 1750
VKEDLLQKKN RGGRKPKRKM KTQKLDADLL VPASVKVLRR SNRKKIDDPI
1760 1770 1780 1790 1800
DEEEEFEELK GSEPHMRTRN QGRRTAFYNE DDSEEEQRQL LFEDTSLTFG
1810 1820 1821
TSSRGRVRKL TEKAKANLIG W

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1363 Collapse assay details

Data source: Panorama

Official Gene Symbol
PHIP
Peptide Modified Sequence
AQS[+80.0]YDIQAWKK
Modification Type
Phospho (ST)
Protein - Site of Modification
1315
Peptide - Site of Modification
3
Peptide Start
1313
Peptide End
1323
CPTAC ID
CPTAC-1363
Peptide Molecular Mass
1,416.6439
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9-98 (2+) 3.2 5 4.8 4.8 8.7 9.9 5.8 10 11 15 15 15
y6 (1+) 4.7 5.7 3.5 8.7 7.1 8.8 9.9 9.1 9.5 15 15 15
y7 (1+) 6.3 4.4 5.5 7.7 7.8 8.2 9.9 9 9.9 15 15 15
sum 3.4 4.2 3.8 5.2 7.7 9.2 6.2 8.8 10 15 15 15


Additional Resources and Comments