PHIP, CPTAC-1363 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PHIP	WDR11

Sequence Length (AA)	Molecular Weight (Da)
1821	206689

Protein Name
PH-interacting protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSCERKGLSE	LRSELYFLIA	RFLEDGPCQQ	AAQVLIREVA	EKELLPRRTD
60	70	80	90	100
WTGKEHPRTY	QNLVKYYRHL	APDHLLQICH	RLGPLLEQEI	PQSVPGVQTL
110	120	130	140	150
LGAGRQSLLR	TNKSCKHVVW	KGSALAALHC	GRPPESPVNY	GSPPSIADTL
160	170	180	190	200
FSRKLNGKYR	LERLVPTAVY	QHMKMHKRIL	GHLSSVYCVT	FDRTGRRIFT
210	220	230	240	250
GSDDCLVKIW	ATDDGRLLAT	LRGHAAEISD	MAVNYENTMI	AAGSCDKMIR
260	270	280	290	300
VWCLRTCAPL	AVLQGHSASI	TSLQFSPLCS	GSKRYLSSTG	ADGTICFWLW
310	320	330	340	350
DAGTLKINPR	PAKFTERPRP	GVQMICSSFS	AGGMFLATGS	TDHIIRVYFF
360	370	380	390	400
GSGQPEKISE	LEFHTDKVDS	IQFSNTSNRF	VSGSRDGTAR	IWQFKRREWK
410	420	430	440	450
SILLDMATRP	AGQNLQGIED	KITKMKVTMV	AWDRHDNTVI	TAVNNMTLKV
460	470	480	490	500
WNSYTGQLIH	VLMGHEDEVF	VLEPHPFDPR	VLFSAGHDGN	VIVWDLARGV
510	520	530	540	550
KIRSYFNMIE	GQGHGAVFDC	KCSPDGQHFA	CTDSHGHLLI	FGFGSSSKYD
560	570	580	590	600
KIADQMFFHS	DYRPLIRDAN	NFVLDEQTQQ	APHLMPPPFL	VDVDGNPHPS
610	620	630	640	650
RYQRLVPGRE	NCREEQLIPQ	MGVTSSGLNQ	VLSQQANQEI	SPLDSMIQRL
660	670	680	690	700
QQEQDLRRSG	EAVISNTSRL	SRGSISSTSE	VHSPPNVGLR	RSGQIEGVRQ
710	720	730	740	750
MHSNAPRSEI	ATERDLVAWS	RRVVVPELSA	GVASRQEEWR	TAKGEEEIKT
760	770	780	790	800
YRSEEKRKHL	TVPKENKIPT	VSKNHAHEHF	LDLGESKKQQ	TNQHNYRTRS
810	820	830	840	850
ALEETPRPSE	EIENGSSSSD	EGEVVAVSGG	TSEEEERAWH	SDGSSSDYSS
860	870	880	890	900
DYSDWTADAG	INLQPPKKVP	KNKTKKAESS	SDEEEESEKQ	KQKQIKKEKK
910	920	930	940	950
KVNEEKDGPI	SPKKKKPKER	KQKRLAVGEL	TENGLTLEEW	LPSTWITDTI
960	970	980	990	1000
PRRCPFVPQM	GDEVYYFRQG	HEAYVEMARK	NKIYSINPKK	QPWHKMELRE
1010	1020	1030	1040	1050
QELMKIVGIK	YEVGLPTLCC	LKLAFLDPDT	GKLTGGSFTM	KYHDMPDVID
1060	1070	1080	1090	1100
FLVLRQQFDD	AKYRRWNIGD	RFRSVIDDAW	WFGTIESQEP	LQLEYPDSLF
1110	1120	1130	1140	1150
QCYNVCWDNG	DTEKMSPWDM	ELIPNNAVFP	EELGTSVPLT	DGECRSLIYK
1160	1170	1180	1190	1200
PLDGEWGTNP	RDEECERIVA	GINQLMTLDI	ASAFVAPVDL	QAYPMYCTVV
1210	1220	1230	1240	1250
AYPTDLSTIK	QRLENRFYRR	VSSLMWEVRY	IEHNTRTFNE	PGSPIVKSAK
1260	1270	1280	1290	1300
FVTDLLLHFI	KDQTCYNIIP	LYNSMKKKVL	SDSEDEEKDA	DVPGTSTRKR
1310	1320	1330	1340	1350
KDHQPRRRLR	NRAQSYDIQA	WKKQCEELLN	LIFQCEDSEP	FRQPVDLLEY
1360	1370	1380	1390	1400
PDYRDIIDTP	MDFATVRETL	EAGNYESPME	LCKDVRLIFS	NSKAYTPSKR
1410	1420	1430	1440	1450
SRIYSMSLRL	SAFFEEHISS	VLSDYKSALR	FHKRNTITKR	RKKRNRSSSV
1460	1470	1480	1490	1500
SSSAASSPER	KKRILKPQLK	SESSTSAFST	PTRSIPPRHN	AAQINGKTES
1510	1520	1530	1540	1550
SSVVRTRSNR	VVVDPVVTEQ	PSTSSAAKTF	ITKANASAIP	GKTILENSVK
1560	1570	1580	1590	1600
HSKALNTLSS	PGQSSFSHGT	RNNSAKENME	KEKPVKRKMK	SSVLPKASTL
1610	1620	1630	1640	1650
SKSSAVIEQG	DCKNNALVPG	TIQVNGHGGQ	PSKLVKRGPG	RKPKVEVNTN
1660	1670	1680	1690	1700
SGEIIHKKRG	RKPKKLQYAK	PEDLEQNNVH	PIRDEVLPSS	TCNFLSETNN
1710	1720	1730	1740	1750
VKEDLLQKKN	RGGRKPKRKM	KTQKLDADLL	VPASVKVLRR	SNRKKIDDPI
1760	1770	1780	1790	1800
DEEEEFEELK	GSEPHMRTRN	QGRRTAFYNE	DDSEEEQRQL	LFEDTSLTFG
1810	1820	1821
TSSRGRVRKL	TEKAKANLIG	W

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1363 Collapse assay details

Data source: Panorama

Official Gene Symbol: PHIP
Peptide Modified Sequence: AQS[+80.0]YDIQAWKK
Modification Type: Phospho (ST)
Protein - Site of Modification: 1315
Peptide - Site of Modification: 3
Peptide Start: 1313
Peptide End: 1323

CPTAC ID: CPTAC-1363
Peptide Molecular Mass: 1,416.6439
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: digested cell lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva
Internal Standard: light stable isotope peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 1.9um, 200A
Column Dimensions: 0.075 x 150 mm
Flow Rate: 200 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Stress Pathway Panel

CPTAC-1342: ACIN1. KIS[+80.0]VVSATK	CPTAC-1343: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPS[+80.0]PR	CPTAC-1344: ARAF. QHEAPSNRPLNELLT[+80.0]PQGPSPR
CPTAC-1345: ARAF. QHEAPSNRPLNELLTPQGPS[+80.0]PR	CPTAC-1346: CIC. AILGS[+80.0]YR	CPTAC-1347: EGFR. ELVEPLT[+80.0]PSGEAPNQALLR
CPTAC-1348: GLYR1. KLS[+80.0]LSEGK	CPTAC-1349: GTF2I. GREFS[+80.0]FEAWNAK	CPTAC-1350: HMGN1. KVS[+80.0]SAEGAAKEEPK
CPTAC-1351: HSPB1. GPS[+80.0]WDPFRDWYPHSR	CPTAC-1352: MAPK1. VADPDHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1353: MAPK1. VADPDHDHTGFLT[+80.0]EYVATR
CPTAC-1354: MAPK1. VADPDHDHTGFLTEY[+80.0]VATR	CPTAC-1355: MAPK14. HTDDEMT[+80.0]GYVATR	CPTAC-1356: MAPK14. HTDDEMTGY[+80.0]VATR
CPTAC-1357: MAPK3. IADPEHDHTGFLT[+80.0]EY[+80.0]VATR	CPTAC-1358: MAPK3. IADPEHDHTGFLT[+80.0]EYVATR	CPTAC-1359: MAPK3. IADPEHDHTGFLTEY[+80.0]VATR
CPTAC-1360: MTOR. KLHVS[+80.0]TINLQK	CPTAC-1361: MTOR. LHVS[+80.0]TINLQK	CPTAC-1362: PBRM1. TYS[+80.0]QDC[+57.0]SFK
CPTAC-1363: PHIP. AQS[+80.0]YDIQAWKK	CPTAC-1364: PRKD2. LGTSES[+80.0]LPC[+57.0]TAEELSR	CPTAC-1365: RBM7. SFS[+80.0]SPENFQR
CPTAC-1366: SHC1. ELFDDPSY[+80.0]VNVQNLDK	CPTAC-1367: SMARCA4. EVDYSDS[+80.0]LTEKQWLK	CPTAC-1368: STMN1. ASGQAFELILS[+80.0]PR
CPTAC-1369: SYNPO2. SLS[+80.0]LPGR	CPTAC-1370: UBE2J1. QIS[+80.0]FKAEVNSSGK	CPTAC-1371: ZNF638. NYQSQADIPIRS[+80.0]PFGIVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9-98 (2+)	3.2	5	4.8	4.8	8.7	9.9	5.8	10	11	15	15	15
y6 (1+)	4.7	5.7	3.5	8.7	7.1	8.8	9.9	9.1	9.5	15	15	15
y7 (1+)	6.3	4.4	5.5	7.7	7.8	8.2	9.9	9	9.9	15	15	15
sum	3.4	4.2	3.8	5.2	7.7	9.2	6.2	8.8	10	15	15	15

PHIP

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1363 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

PHIP

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1363 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments