Overview Data source: UniProt

Official Gene Symbol Other Aliases
AURKA AIK,AIRK1,ARK1,AURA,AYK1,BTAK,IAK1,STK15,STK6
Sequence Length (AA) Molecular Weight (Da)
403 45809
Protein Name
Aurora kinase A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP
60 70 80 90 100
SNSSQRVPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ
110 120 130 140 150
PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA
160 170 180 190 200
REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF
210 220 230 240 250
HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK
260 270 280 290 300
RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM
310 320 330 340 350
IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD
360 370 380 390 400
FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS
403
KQS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1341 Collapse assay details

Data source: Panorama

Official Gene Symbol
AURKA
Peptide Modified Sequence
TT[+80.0]LC[+57.0]GTLDYLPPEMIEGR
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
288, 290
Peptide - Site of Modification
2, 4
Peptide Start
287
Peptide End
304
CPTAC ID
CPTAC-1341
Peptide Molecular Mass
2,144.9523
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 23.5 8.3 7.4 26.1 8.5 11.5 35.1 11.9 13.7 15 15 15
y7 (1+) 29.3 17.4 15.6 29.6 19.4 19.1 41.6 26.1 24.7 15 15 15
y8 (2+) 18.9 4.3 4 21.3 5.2 9.8 28.5 6.7 10.6 15 15 15
sum 17.9 3.7 3.7 20.6 3.8 10 27.3 5.3 10.7 15 15 15

Additional Resources and Comments