SUFU, CPTAC-1329 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SUFU	N/A

Sequence Length (AA)	Molecular Weight (Da)
484	53947

Protein Name
Suppressor of fused homolog

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAELRPSGAP	GPTAPPAPGP	TAPPAFASLF	PPGLHAIYGE	CRRLYPDQPN
60	70	80	90	100
PLQVTAIVKY	WLGGPDPLDY	VSMYRNVGSP	SANIPEHWHY	ISFGLSDLYG
110	120	130	140	150
DNRVHEFTGT	DGPSGFGFEL	TFRLKRETGE	SAPPTWPAEL	MQGLARYVFQ
160	170	180	190	200
SENTFCSGDH	VSWHSPLDNS	ESRIQHMLLT	EDPQMQPVQT	PFGVVTFLQI
210	220	230	240	250
VGVCTEELHS	AQQWNGQGIL	ELLRTVPIAG	GPWLITDMRR	GETIFEIDPH
260	270	280	290	300
LQERVDKGIE	TDGSNLSGVS	AKCAWDDLSR	PPEDDEDSRS	ICIGTQPRRL
310	320	330	340	350
SGKDTEQIRE	TLRRGLEINS	KPVLPPINPQ	RQNGLAHDRA	PSRKDSLESD
360	370	380	390	400
SSTAIIPHEL	IRTRQLESVH	LKFNQESGAL	IPLCLRGRLL	HGRHFTYKSI
410	420	430	440	450
TGDMAITFVS	TGVEGAFATE	EHPYAAHGPW	LQILLTEEFV	EKMLEDLEDL
460	470	480	484
TSPEEFKLPK	EYSWPEKKLK	VSILPDVVFD	SPLH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1329 Collapse assay details

Data source: Panorama

Official Gene Symbol: SUFU
Peptide Modified Sequence: DS[+80.0]LESDSSTAIIPHELIR
Modification Type: Phospho (ST)
Protein - Site of Modification: 346
Peptide - Site of Modification: 2
Peptide Start: 345
Peptide End: 362

CPTAC ID: CPTAC-1329
Peptide Molecular Mass: 2,061.9620
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways

CPTAC-1323: FOXO1. TSS[+80.0]NASTISGR	CPTAC-1324: JUN. NSDLLTS[+80.0]PDVGLLK	CPTAC-1325: MAPK14. HTDDEMT[+80.0]GYVATR
CPTAC-1326: PLK1. RS[+80.0]LLELHK	CPTAC-1327: PLK1. KT[+80.0]LC[+57.0]GTPNYIAPEVLSK	CPTAC-1328: RB1. TAVIPINGS[+80.0]PR
CPTAC-1329: SUFU. DS[+80.0]LESDSSTAIIPHELIR	CPTAC-1330: NFKB1. DSDSVC[+57.0]DSGVETS[+80.0]FR	CPTAC-1331: RPS6. LS[+80.0]SLRASTSK
CPTAC-1332: RPS6. LSS[+80.0]LRASTSK	CPTAC-1333: RPS6. LSSLRAS[+80.0]TSK	CPTAC-1334: TP53BP1. QYTES[+80.0]QLR
CPTAC-1335: RAF1. AS[+80.0]DDGKLTDPSK	CPTAC-1336: RAF1. SAS[+80.0]EPSLHR	CPTAC-1337: RICTOR. AQS[+80.0]LKAPSIATIK
CPTAC-1338: PRKCB. QPVELT[+80.0]PTDK	CPTAC-1339: ARAF. DS[+80.0]GYYWEVPPSEVQLLK	CPTAC-1340: AURKB. KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
CPTAC-1341: AURKA. TT[+80.0]LC[+57.0]GTLDYLPPEMIEGR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	21	11.9	7.2	15.7	17	10.2	26.2	20.8	12.5	15	15	15
y6 (1+)	10.4	5	4.3	8.1	9.3	9.3	13.2	10.6	10.2	15	15	15
b4-98 (1+)	14.2	8.1	10.9	11.5	7.9	17.7	18.3	11.3	20.8	15	15	15
sum	10.9	4.4	4.7	8.1	8.3	9.7	13.6	9.4	10.8	15	15	15

SUFU

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1329 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SUFU

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1329 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments