Overview Data source: UniProt

Official Gene Symbol Other Aliases
RPS6 N/A
Sequence Length (AA) Molecular Weight (Da)
249 28681
Protein Name
40S ribosomal protein S6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV
60 70 80 90 100
RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRTG ERKRKSVRGC
110 120 130 140 150
IVDANLSVLN LVIVKKGEKD IPGLTDTTVP RRLGPKRASR IRKLFNLSKE
160 170 180 190 200
DDVRQYVVRK PLNKEGKKPR TKAPKIQRLV TPRVLQHKRR RIALKKQRTK
210 220 230 240 249
KNKEEAAEYA KLLAKRMKEA KEKRQEQIAK RRRLSSLRAS TSKSESSQK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1331 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPS6
Peptide Modified Sequence
LS[+80.0]SLRASTSK
Modification Type
Phospho (ST)
Protein - Site of Modification
235
Peptide - Site of Modification
2
Peptide Start
234
Peptide End
243
CPTAC ID
CPTAC-1331
Peptide Molecular Mass
1,128.5540
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 18.1 15.8 14.5 20.4 13.9 12.4 27.3 21 19.1 15 15 15
sum 18.1 15.8 14.5 20.4 13.9 12.4 27.3 21 19.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1332 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPS6
Peptide Modified Sequence
LSS[+80.0]LRASTSK
Modification Type
Phospho (ST)
Protein - Site of Modification
236
Peptide - Site of Modification
3
Peptide Start
234
Peptide End
243
CPTAC ID
CPTAC-1332
Peptide Molecular Mass
1,128.5540
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 18.5 14.9 14.6 23.2 17.4 19.1 29.7 22.9 24 15 15 15
sum 18.5 14.9 14.6 23.2 17.4 19.1 29.7 22.9 24 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1333 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPS6
Peptide Modified Sequence
LSSLRAS[+80.0]TSK
Modification Type
Phospho (ST)
Protein - Site of Modification
240
Peptide - Site of Modification
7
Peptide Start
234
Peptide End
243
CPTAC ID
CPTAC-1333
Peptide Molecular Mass
1,128.5540
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 14 13 14.8 15.3 19.9 16.6 20.7 23.8 22.2 15 15 15
y7 (1+) 52.8 50.1 56.9 66 66.3 66.3 84.5 83.1 87.4 15 15 15
b9-98 (1+) 23.5 16.7 12.3 26.8 19.2 15.8 35.6 25.4 20 15 15 15
sum 11.5 11.1 9.9 11.6 15 12.4 16.3 18.7 15.9 15 15 15

Additional Resources and Comments