RPS6, CPTAC-1333 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RPS6	N/A

Sequence Length (AA)	Molecular Weight (Da)
249	28681

Protein Name
40S ribosomal protein S6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKLNISFPAT	GCQKLIEVDD	ERKLRTFYEK	RMATEVAADA	LGEEWKGYVV
60	70	80	90	100
RISGGNDKQG	FPMKQGVLTH	GRVRLLLSKG	HSCYRPRRTG	ERKRKSVRGC
110	120	130	140	150
IVDANLSVLN	LVIVKKGEKD	IPGLTDTTVP	RRLGPKRASR	IRKLFNLSKE
160	170	180	190	200
DDVRQYVVRK	PLNKEGKKPR	TKAPKIQRLV	TPRVLQHKRR	RIALKKQRTK
210	220	230	240	249
KNKEEAAEYA	KLLAKRMKEA	KEKRQEQIAK	RRRLSSLRAS	TSKSESSQK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1331 Collapse assay details

Data source: Panorama

Official Gene Symbol: RPS6
Peptide Modified Sequence: LS[+80.0]SLRASTSK
Modification Type: Phospho (ST)
Protein - Site of Modification: 235
Peptide - Site of Modification: 2
Peptide Start: 234
Peptide End: 243

CPTAC ID: CPTAC-1331
Peptide Molecular Mass: 1,128.5540
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

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Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways

CPTAC-1323: FOXO1. TSS[+80.0]NASTISGR	CPTAC-1324: JUN. NSDLLTS[+80.0]PDVGLLK	CPTAC-1325: MAPK14. HTDDEMT[+80.0]GYVATR
CPTAC-1326: PLK1. RS[+80.0]LLELHK	CPTAC-1327: PLK1. KT[+80.0]LC[+57.0]GTPNYIAPEVLSK	CPTAC-1328: RB1. TAVIPINGS[+80.0]PR
CPTAC-1329: SUFU. DS[+80.0]LESDSSTAIIPHELIR	CPTAC-1330: NFKB1. DSDSVC[+57.0]DSGVETS[+80.0]FR	CPTAC-1331: RPS6. LS[+80.0]SLRASTSK
CPTAC-1332: RPS6. LSS[+80.0]LRASTSK	CPTAC-1333: RPS6. LSSLRAS[+80.0]TSK	CPTAC-1334: TP53BP1. QYTES[+80.0]QLR
CPTAC-1335: RAF1. AS[+80.0]DDGKLTDPSK	CPTAC-1336: RAF1. SAS[+80.0]EPSLHR	CPTAC-1337: RICTOR. AQS[+80.0]LKAPSIATIK
CPTAC-1338: PRKCB. QPVELT[+80.0]PTDK	CPTAC-1339: ARAF. DS[+80.0]GYYWEVPPSEVQLLK	CPTAC-1340: AURKB. KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
CPTAC-1341: AURKA. TT[+80.0]LC[+57.0]GTLDYLPPEMIEGR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	18.1	15.8	14.5	20.4	13.9	12.4	27.3	21	19.1	15	15	15
sum	18.1	15.8	14.5	20.4	13.9	12.4	27.3	21	19.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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