RICTOR, CPTAC-1337 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RICTOR	KIAA1999

Sequence Length (AA)	Molecular Weight (Da)
1708	192218

Protein Name
Rapamycin-insensitive companion of mTOR

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAIGRGRSL	KNLRVRGRND	SGEENVPLDL	TREPSDNLRE	ILQNVARLQG
60	70	80	90	100
VSNMRKLGHL	NNFTKLLCDI	GHSEEKLGFH	YEDIIICLRL	ALLNEAKEVR
110	120	130	140	150
AAGLRALRYL	IQDSSILQKV	LKLKVDYLIA	RCIDIQQSNE	VERTQALRLV
160	170	180	190	200
RKMITVNASL	FPSSVTNSLI	AVGNDGLQER	DRMVRACIAI	ICELALQNPE
210	220	230	240	250
VVALRGGLNT	ILKNVIDCQL	SRINEALITT	ILHLLNHPKT	RQYVRADVEL
260	270	280	290	300
ERILAPYTDF	HYRHSPDTAE	GQLKEDREAR	FLASKMGIIA	TFRSWAGIIN
310	320	330	340	350
LCKPGNSGIQ	SLIGVLCIPN	MEIRRGLLEV	LYDIFRLPLP	VVTEEFIEAL
360	370	380	390	400
LSVDPGRFQD	SWRLSDGFVA	AEAKTILPHR	ARSRPDLMDN	YLALILSAFI
410	420	430	440	450
RNGLLEGLVE	VITNSDDHIS	VRATILLGEL	LHMANTILPH	SHSHHLHCLP
460	470	480	490	500
TLMNMAASFD	IPKEKRLRAS	AALNCLKRFH	EMKKRGPKPY	SLHLDHIIQK
510	520	530	540	550
AIATHQKRDQ	YLRVQKDIFI	LKDTEEALLI	NLRDSQVLQH	KENLEWNWNL
560	570	580	590	600
IGTILKWPNV	NLRNYKDEQL	HRFVRRLLYF	YKPSSKLYAN	LDLDFAKAKQ
610	620	630	640	650
LTVVGCQFTE	FLLESEEDGQ	GYLEDLVKDI	VQWLNASSGM	KPERSLQNNG
660	670	680	690	700
LLTTLSQHYF	LFIGTLSCHP	HGVKMLEKCS	VFQCLLNLCS	LKNQDHLLKL
710	720	730	740	750
TVSSLDYSRD	GLARVILSKI	LTAATDACRL	YATKHLRVLL	RANVEFFNNW
760	770	780	790	800
GIELLVTQLH	DKNKTISSEA	LDILDEACED	KANLHALIQM	KPALSHLGDK
810	820	830	840	850
GLLLLLRFLS	IPKGFSYLNE	RGYVAKQLEK	WHREYNSKYV	DLIEEQLNEA
860	870	880	890	900
LTTYRKPVDG	DNYVRRSNQR	LQRPHVYLPI	HLYGQLVHHK	TGCHLLEVQN
910	920	930	940	950
IITELCRNVR	TPDLDKWEEI	KKLKASLWAL	GNIGSSNWGL	NLLQEENVIP
960	970	980	990	1000
DILKLAKQCE	VLSIRGTCVY	VLGLIAKTKQ	GCDILKCHNW	DAVRHSRKHL
1010	1020	1030	1040	1050
WPVVPDDVEQ	LCNELSSIPS	TLSLNSESTS	SRHNSESESV	PSSMFILEDD
1060	1070	1080	1090	1100
RFGSSSTSTF	FLDINEDTEP	TFYDRSGPIK	DKNSFPFFAS	SKLVKNRILN
1110	1120	1130	1140	1150
SLTLPNKKHR	SSSDPKGGKL	SSESKTSNRR	IRTLTEPSVD	FNHSDDFTPI
1160	1170	1180	1190	1200
STVQKTLQLE	TSFMGNKHIE	DTGSTPSIGE	NDLKFTKNFG	TENHRENTSR
1210	1220	1230	1240	1250
ERLVVESSTS	SHMKIRSQSF	NTDTTTSGIS	SMSSSPSRET	VGVDATTMDT
1260	1270	1280	1290	1300
DCGSMSTVVS	TKTIKTSHYL	TPQSNHLSLS	KSNSVSLVPP	GSSHTLPRRA
1310	1320	1330	1340	1350
QSLKAPSIAT	IKSLADCNFS	YTSSRDAFGY	ATLKRLQQQR	MHPSLSHSEA
1360	1370	1380	1390	1400
LASPAKDVLF	TDTITMKANS	FESRLTPSRF	MKALSYASLD	KEDLLSPINQ
1410	1420	1430	1440	1450
NTLQRSSSVR	SMVSSATYGG	SDDYIGLALP	VDINDIFQVK	DIPYFQTKNI
1460	1470	1480	1490	1500
PPHDDRGARA	FAHDAGGLPS	GTGGLVKNSF	HLLRQQMSLT	EIMNSIHSDA
1510	1520	1530	1540	1550
SLFLESTEDT	GLQEHTDDNC	LYCVCIEILG	FQPSNQLSAI	CSHSDFQDIP
1560	1570	1580	1590	1600
YSDWCEQTIH	NPLEVVPSKF	SGISGCSDGV	SQEGSASSTK	STELLLGVKT
1610	1620	1630	1640	1650
IPDDTPMCRI	LLRKEVLRLV	INLSSSVSTK	CHETGLLTIK	EKYPQTFDDI
1660	1670	1680	1690	1700
CLYSEVSHLL	SHCTFRLPCR	RFIQELFQDV	QFLQMHEEAE	AVLATPPKQP
1708
IVDTSAES

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1337 Collapse assay details

Data source: Panorama

Official Gene Symbol: RICTOR
Peptide Modified Sequence: AQS[+80.0]LKAPSIATIK
Modification Type: Phospho (ST)
Protein - Site of Modification: 1302
Peptide - Site of Modification: 3
Peptide Start: 1300
Peptide End: 1312

CPTAC ID: CPTAC-1337
Peptide Molecular Mass: 1,406.7534
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways

CPTAC-1323: FOXO1. TSS[+80.0]NASTISGR	CPTAC-1324: JUN. NSDLLTS[+80.0]PDVGLLK	CPTAC-1325: MAPK14. HTDDEMT[+80.0]GYVATR
CPTAC-1326: PLK1. RS[+80.0]LLELHK	CPTAC-1327: PLK1. KT[+80.0]LC[+57.0]GTPNYIAPEVLSK	CPTAC-1328: RB1. TAVIPINGS[+80.0]PR
CPTAC-1329: SUFU. DS[+80.0]LESDSSTAIIPHELIR	CPTAC-1330: NFKB1. DSDSVC[+57.0]DSGVETS[+80.0]FR	CPTAC-1331: RPS6. LS[+80.0]SLRASTSK
CPTAC-1332: RPS6. LSS[+80.0]LRASTSK	CPTAC-1333: RPS6. LSSLRAS[+80.0]TSK	CPTAC-1334: TP53BP1. QYTES[+80.0]QLR
CPTAC-1335: RAF1. AS[+80.0]DDGKLTDPSK	CPTAC-1336: RAF1. SAS[+80.0]EPSLHR	CPTAC-1337: RICTOR. AQS[+80.0]LKAPSIATIK
CPTAC-1338: PRKCB. QPVELT[+80.0]PTDK	CPTAC-1339: ARAF. DS[+80.0]GYYWEVPPSEVQLLK	CPTAC-1340: AURKB. KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
CPTAC-1341: AURKA. TT[+80.0]LC[+57.0]GTLDYLPPEMIEGR

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	16.6	19.5	15.3	19.3	20.6	19	25.5	28.4	24.4	15	15	15
y7 (1+)	20.1	7.2	10.8	12.9	12.3	12.8	23.9	14.3	16.7	15	15	15
y4 (1+)	12.5	10.6	5.8	14.5	11.8	5.5	19.1	15.9	8	15	15	15
sum	11.4	8.7	2.7	10.1	11.7	4.5	15.2	14.6	5.2	15	15	15

RICTOR

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1337 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

RICTOR

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1337 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments