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Overview Data source: UniProt

Official Gene Symbol Other Aliases
RICTOR KIAA1999
Sequence Length (AA) Molecular Weight (Da)
1708 192218
Protein Name
Rapamycin-insensitive companion of mTOR
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAIGRGRSL KNLRVRGRND SGEENVPLDL TREPSDNLRE ILQNVARLQG
60 70 80 90 100
VSNMRKLGHL NNFTKLLCDI GHSEEKLGFH YEDIIICLRL ALLNEAKEVR
110 120 130 140 150
AAGLRALRYL IQDSSILQKV LKLKVDYLIA RCIDIQQSNE VERTQALRLV
160 170 180 190 200
RKMITVNASL FPSSVTNSLI AVGNDGLQER DRMVRACIAI ICELALQNPE
210 220 230 240 250
VVALRGGLNT ILKNVIDCQL SRINEALITT ILHLLNHPKT RQYVRADVEL
260 270 280 290 300
ERILAPYTDF HYRHSPDTAE GQLKEDREAR FLASKMGIIA TFRSWAGIIN
310 320 330 340 350
LCKPGNSGIQ SLIGVLCIPN MEIRRGLLEV LYDIFRLPLP VVTEEFIEAL
360 370 380 390 400
LSVDPGRFQD SWRLSDGFVA AEAKTILPHR ARSRPDLMDN YLALILSAFI
410 420 430 440 450
RNGLLEGLVE VITNSDDHIS VRATILLGEL LHMANTILPH SHSHHLHCLP
460 470 480 490 500
TLMNMAASFD IPKEKRLRAS AALNCLKRFH EMKKRGPKPY SLHLDHIIQK
510 520 530 540 550
AIATHQKRDQ YLRVQKDIFI LKDTEEALLI NLRDSQVLQH KENLEWNWNL
560 570 580 590 600
IGTILKWPNV NLRNYKDEQL HRFVRRLLYF YKPSSKLYAN LDLDFAKAKQ
610 620 630 640 650
LTVVGCQFTE FLLESEEDGQ GYLEDLVKDI VQWLNASSGM KPERSLQNNG
660 670 680 690 700
LLTTLSQHYF LFIGTLSCHP HGVKMLEKCS VFQCLLNLCS LKNQDHLLKL
710 720 730 740 750
TVSSLDYSRD GLARVILSKI LTAATDACRL YATKHLRVLL RANVEFFNNW
760 770 780 790 800
GIELLVTQLH DKNKTISSEA LDILDEACED KANLHALIQM KPALSHLGDK
810 820 830 840 850
GLLLLLRFLS IPKGFSYLNE RGYVAKQLEK WHREYNSKYV DLIEEQLNEA
860 870 880 890 900
LTTYRKPVDG DNYVRRSNQR LQRPHVYLPI HLYGQLVHHK TGCHLLEVQN
910 920 930 940 950
IITELCRNVR TPDLDKWEEI KKLKASLWAL GNIGSSNWGL NLLQEENVIP
960 970 980 990 1000
DILKLAKQCE VLSIRGTCVY VLGLIAKTKQ GCDILKCHNW DAVRHSRKHL
1010 1020 1030 1040 1050
WPVVPDDVEQ LCNELSSIPS TLSLNSESTS SRHNSESESV PSSMFILEDD
1060 1070 1080 1090 1100
RFGSSSTSTF FLDINEDTEP TFYDRSGPIK DKNSFPFFAS SKLVKNRILN
1110 1120 1130 1140 1150
SLTLPNKKHR SSSDPKGGKL SSESKTSNRR IRTLTEPSVD FNHSDDFTPI
1160 1170 1180 1190 1200
STVQKTLQLE TSFMGNKHIE DTGSTPSIGE NDLKFTKNFG TENHRENTSR
1210 1220 1230 1240 1250
ERLVVESSTS SHMKIRSQSF NTDTTTSGIS SMSSSPSRET VGVDATTMDT
1260 1270 1280 1290 1300
DCGSMSTVVS TKTIKTSHYL TPQSNHLSLS KSNSVSLVPP GSSHTLPRRA
1310 1320 1330 1340 1350
QSLKAPSIAT IKSLADCNFS YTSSRDAFGY ATLKRLQQQR MHPSLSHSEA
1360 1370 1380 1390 1400
LASPAKDVLF TDTITMKANS FESRLTPSRF MKALSYASLD KEDLLSPINQ
1410 1420 1430 1440 1450
NTLQRSSSVR SMVSSATYGG SDDYIGLALP VDINDIFQVK DIPYFQTKNI
1460 1470 1480 1490 1500
PPHDDRGARA FAHDAGGLPS GTGGLVKNSF HLLRQQMSLT EIMNSIHSDA
1510 1520 1530 1540 1550
SLFLESTEDT GLQEHTDDNC LYCVCIEILG FQPSNQLSAI CSHSDFQDIP
1560 1570 1580 1590 1600
YSDWCEQTIH NPLEVVPSKF SGISGCSDGV SQEGSASSTK STELLLGVKT
1610 1620 1630 1640 1650
IPDDTPMCRI LLRKEVLRLV INLSSSVSTK CHETGLLTIK EKYPQTFDDI
1660 1670 1680 1690 1700
CLYSEVSHLL SHCTFRLPCR RFIQELFQDV QFLQMHEEAE AVLATPPKQP
1708
IVDTSAES

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1337 Collapse assay details

Data source: Panorama

Official Gene Symbol
RICTOR
Peptide Modified Sequence
AQS[+80.0]LKAPSIATIK
Modification Type
Phospho (ST)
Protein - Site of Modification
1302
Peptide - Site of Modification
3
Peptide Start
1300
Peptide End
1312
CPTAC ID
CPTAC-1337
Peptide Molecular Mass
1,406.7534
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 16.6 19.5 15.3 19.3 20.6 19 25.5 28.4 24.4 15 15 15
y7 (1+) 20.1 7.2 10.8 12.9 12.3 12.8 23.9 14.3 16.7 15 15 15
y4 (1+) 12.5 10.6 5.8 14.5 11.8 5.5 19.1 15.9 8 15 15 15
sum 11.4 8.7 2.7 10.1 11.7 4.5 15.2 14.6 5.2 15 15 15

Additional Resources and Comments