PRKCB, CPTAC-1338 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKCB	PKCB, PRKCB1

Sequence Length (AA)	Molecular Weight (Da)
671	76869

Protein Name
Protein kinase C beta type

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MADPAAGPPP	SEGEESTVRF	ARKGALRQKN	VHEVKNHKFT	ARFFKQPTFC
60	70	80	90	100
SHCTDFIWGF	GKQGFQCQVC	CFVVHKRCHE	FVTFSCPGAD	KGPASDDPRS
110	120	130	140	150
KHKFKIHTYS	SPTFCDHCGS	LLYGLIHQGM	KCDTCMMNVH	KRCVMNVPSL
160	170	180	190	200
CGTDHTERRG	RIYIQAHIDR	DVLIVLVRDA	KNLVPMDPNG	LSDPYVKLKL
210	220	230	240	250
IPDPKSESKQ	KTKTIKCSLN	PEWNETFRFQ	LKESDKDRRL	SVEIWDWDLT
260	270	280	290	300
SRNDFMGSLS	FGISELQKAS	VDGWFKLLSQ	EEGEYFNVPV	PPEGSEANEE
310	320	330	340	350
LRQKFERAKI	SQGTKVPEEK	TTNTVSKFDN	NGNRDRMKLT	DFNFLMVLGK
360	370	380	390	400
GSFGKVMLSE	RKGTDELYAV	KILKKDVVIQ	DDDVECTMVE	KRVLALPGKP
410	420	430	440	450
PFLTQLHSCF	QTMDRLYFVM	EYVNGGDLMY	HIQQVGRFKE	PHAVFYAAEI
460	470	480	490	500
AIGLFFLQSK	GIIYRDLKLD	NVMLDSEGHI	KIADFGMCKE	NIWDGVTTKT
510	520	530	540	550
FCGTPDYIAP	EIIAYQPYGK	SVDWWAFGVL	LYEMLAGQAP	FEGEDEDELF
560	570	580	590	600
QSIMEHNVAY	PKSMSKEAVA	ICKGLMTKHP	GKRLGCGPEG	ERDIKEHAFF
610	620	630	640	650
RYIDWEKLER	KEIQPPYKPK	ARDKRDTSNF	DKEFTRQPVE	LTPTDKLFIM
660	670	671
NLDQNEFAGF	SYTNPEFVIN	V

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1338 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCB
Peptide Modified Sequence: QPVELT[+80.0]PTDK
Modification Type: Phospho (ST)
Protein - Site of Modification: 642
Peptide - Site of Modification: 6
Peptide Start: 637
Peptide End: 646

CPTAC ID: CPTAC-1338
Peptide Molecular Mass: 1,206.5533
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: Fe(III)-NTA
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways

CPTAC-1323: FOXO1. TSS[+80.0]NASTISGR	CPTAC-1324: JUN. NSDLLTS[+80.0]PDVGLLK	CPTAC-1325: MAPK14. HTDDEMT[+80.0]GYVATR
CPTAC-1326: PLK1. RS[+80.0]LLELHK	CPTAC-1327: PLK1. KT[+80.0]LC[+57.0]GTPNYIAPEVLSK	CPTAC-1328: RB1. TAVIPINGS[+80.0]PR
CPTAC-1329: SUFU. DS[+80.0]LESDSSTAIIPHELIR	CPTAC-1330: NFKB1. DSDSVC[+57.0]DSGVETS[+80.0]FR	CPTAC-1331: RPS6. LS[+80.0]SLRASTSK
CPTAC-1332: RPS6. LSS[+80.0]LRASTSK	CPTAC-1333: RPS6. LSSLRAS[+80.0]TSK	CPTAC-1334: TP53BP1. QYTES[+80.0]QLR
CPTAC-1335: RAF1. AS[+80.0]DDGKLTDPSK	CPTAC-1336: RAF1. SAS[+80.0]EPSLHR	CPTAC-1337: RICTOR. AQS[+80.0]LKAPSIATIK
CPTAC-1338: PRKCB. QPVELT[+80.0]PTDK	CPTAC-1339: ARAF. DS[+80.0]GYYWEVPPSEVQLLK	CPTAC-1340: AURKB. KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
CPTAC-1341: AURKA. TT[+80.0]LC[+57.0]GTLDYLPPEMIEGR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	48.5	29	33.5	54.7	28.5	47.3	73.1	40.7	58	15	15	15
y5 (1+)	45.7	35.9	24.9	78.2	37.6	53.9	90.6	52	59.4	15	15	15
y4 (1+)	23.2	20.4	15.4	25	21.5	16.2	34.1	29.6	22.4	15	15	15
sum	20.4	10.7	15	18.8	12.4	20.3	27.7	16.4	25.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3052 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCB
Peptide Sequence: ENIWDGVTTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 490
Peptide End: 499

CPTAC ID: CPTAC-3052
Peptide Molecular Mass: 1,161.5666
Species: Homo Sapiens
Assay Type: Direct MRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend