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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AURKB AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5
Sequence Length (AA) Molecular Weight (Da)
344 39311
Protein Name
Aurora kinase B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT
60 70 80 90 100
AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH
110 120 130 140 150
FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI
160 170 180 190 200
YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD
210 220 230 240 250
IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH
260 270 280 290 300
NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPMGA
310 320 330 340 344
QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1340 Collapse assay details

Data source: Panorama

Official Gene Symbol
AURKB
Peptide Modified Sequence
KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
232, 234
Peptide - Site of Modification
2, 4
Peptide Start
231
Peptide End
248
CPTAC ID
CPTAC-1340
Peptide Molecular Mass
2,189.9561
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Fe(III)-NTA
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 17.6 6.6 4.9 17.5 8 8.4 24.8 10.4 9.7 15 15 15
y7 (1+) 29.4 9.4 11.9 28.3 14.7 15.8 40.8 17.4 19.8 15 15 15
y8 (2+) 4.3 5.2 3.9 5 6.2 9.7 6.6 8.1 10.5 15 15 15
sum 6.2 4.3 3.2 7.8 5.5 8.9 10 7 9.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1219 Collapse assay details

Data source: Panorama

Official Gene Symbol
AURKB
Peptide Sequence
LPLAQVSAHPWVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
316
Peptide End
328
CPTAC ID
CPTAC-1219
Peptide Molecular Mass
1,472.8252
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 31.7 7.6 7 32.2 12.3 18.9 45.2 14.5 20.2 15 15 15
y7 (1+) 41.4 9.1 8 51.2 20.4 15.7 65.8 22.3 17.6 15 15 15
y8 (1+) 65 21.9 10.3 68.8 23.6 15.8 94.6 32.2 18.9 15 15 15
sum 27.3 7.2 4.9 31.1 14.8 16.3 41.4 16.5 17 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1228 Collapse assay details

Data source: Panorama

Official Gene Symbol
AURKB
Peptide Sequence
SNVQPTAAPGQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
45
Peptide End
56
CPTAC ID
CPTAC-1228
Peptide Molecular Mass
1,196.6150
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Broad Institute-Biologist curated set of targets across a number of important cancer pathways


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 34.7 11.7 17.9 49.5 14.5 18.4 60.5 18.6 25.7 15 15 15
y5 (1+) 67.3 15.6 19.7 78.2 16.1 20.6 103.2 22.4 28.5 15 15 15
y8 (1+) 29.3 17.9 15.5 36.5 23.8 18 46.8 29.8 23.8 15 15 15
sum 25.8 14.1 14.4 28.9 19.8 16.5 38.7 24.3 21.9 15 15 15

Additional Resources and Comments