AURKB, CPTAC-3030 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AURKB	AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5

Sequence Length (AA)	Molecular Weight (Da)
344	39311

Protein Name
Aurora kinase B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAQKENSYPW	PYGRQTAPSG	LSTLPQRVLR	KEPVTPSALV	LMSRSNVQPT
60	70	80	90	100
AAPGQKVMEN	SSGTPDILTR	HFTIDDFEIG	RPLGKGKFGN	VYLAREKKSH
110	120	130	140	150
FIVALKVLFK	SQIEKEGVEH	QLRREIEIQA	HLHHPNILRL	YNYFYDRRRI
160	170	180	190	200
YLILEYAPRG	ELYKELQKSC	TFDEQRTATI	MEELADALMY	CHGKKVIHRD
210	220	230	240	250
IKPENLLLGL	KGELKIADFG	WSVHAPSLRR	KTMCGTLDYL	PPEMIEGRMH
260	270	280	290	300
NEKVDLWCIG	VLCYELLVGN	PPFESASHNE	TYRRIVKVDL	KFPASVPMGA
310	320	330	340	344
QDLISKLLRH	NPSERLPLAQ	VSAHPWVRAN	SRRVLPPSAL	QSVA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1340 Collapse assay details

Data source: Panorama

Official Gene Symbol: AURKB
Peptide Modified Sequence: KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
Modification Type: Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification: 232, 234
Peptide - Site of Modification: 2, 4
Peptide Start: 231
Peptide End: 248

CPTAC ID: CPTAC-1340
Peptide Molecular Mass: 2,189.9561
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: N/A
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	17.6	6.6	4.9	17.5	8	8.4	24.8	10.4	9.7	15	15	15
y7 (1+)	29.4	9.4	11.9	28.3	14.7	15.8	40.8	17.4	19.8	15	15	15
y8 (2+)	4.3	5.2	3.9	5	6.2	9.7	6.6	8.1	10.5	15	15	15
sum	6.2	4.3	3.2	7.8	5.5	8.9	10	7	9.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-1219 Collapse assay details

Data source: Panorama

Official Gene Symbol: AURKB
Peptide Sequence: LPLAQVSAHPWVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 316
Peptide End: 328

CPTAC ID: CPTAC-1219
Peptide Molecular Mass: 1,472.8252
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu