AURKA, CPTAC-3083 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AURKA	AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6

Sequence Length (AA)	Molecular Weight (Da)
403	45809

Protein Name
Aurora kinase A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDRSKENCIS	GPVKATAPVG	GPKRVLVTQQ	FPCQNPLPVN	SGQAQRVLCP
60	70	80	90	100
SNSSQRVPLQ	AQKLVSSHKP	VQNQKQKQLQ	ATSVPHPVSR	PLNNTQKSKQ
110	120	130	140	150
PLPSAPENNP	EEELASKQKN	EESKKRQWAL	EDFEIGRPLG	KGKFGNVYLA
160	170	180	190	200
REKQSKFILA	LKVLFKAQLE	KAGVEHQLRR	EVEIQSHLRH	PNILRLYGYF
210	220	230	240	250
HDATRVYLIL	EYAPLGTVYR	ELQKLSKFDE	QRTATYITEL	ANALSYCHSK
260	270	280	290	300
RVIHRDIKPE	NLLLGSAGEL	KIADFGWSVH	APSSRRTTLC	GTLDYLPPEM
310	320	330	340	350
IEGRMHDEKV	DLWSLGVLCY	EFLVGKPPFE	ANTYQETYKR	ISRVEFTFPD
360	370	380	390	400
FVTEGARDLI	SRLLKHNPSQ	RPMLREVLEH	PWITANSSKP	SNCQNKESAS
403
KQS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1341 Collapse assay details

Data source: Panorama

Official Gene Symbol: AURKA
Peptide Modified Sequence: TT[+80.0]LC[+57.0]GTLDYLPPEMIEGR
Modification Type: Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification: 288, 290
Peptide - Site of Modification: 2, 4
Peptide Start: 287
Peptide End: 304

CPTAC ID: CPTAC-1341
Peptide Molecular Mass: 2,144.9523
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: N/A
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	23.5	8.3	7.4	26.1	8.5	11.5	35.1	11.9	13.7	15	15	15
y7 (1+)	29.3	17.4	15.6	29.6	19.4	19.1	41.6	26.1	24.7	15	15	15
y8 (2+)	18.9	4.3	4	21.3	5.2	9.8	28.5	6.7	10.6	15	15	15
sum	17.9	3.7	3.7	20.6	3.8	10	27.3	5.3	10.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3084 Collapse assay details

Data source: Panorama

Official Gene Symbol: AURKA
Peptide Sequence: DIKPENLLLGSAGELK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 256
Peptide End: 271

CPTAC ID: CPTAC-3084
Peptide Molecular Mass: 1,695.9407
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend