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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Aplp1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Aplp1 N/A
Sequence Length (AA) Molecular Weight (Da)
653 72751
Protein Name
Amyloid-like protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPTSPAARG QGRRWRPPLP LLLPLSLLLL RAQLAVGNLA VGSPSAAEAP
60 70 80 90 100
GSAQVAGLCG RLTLHRDLRT GRWEPDPQRS RRCLLDPQRV LEYCRQMYPE
110 120 130 140 150
LHIARVEQAA QAIPMERWCG GTRSGRCAHP HHEVVPFHCL PGEFVSEALL
160 170 180 190 200
VPEGCRFLHQ ERMDQCESST RRHQEAQEAC SSQGLILHGS GMLLPCGSDR
210 220 230 240 250
FRGVEYVCCP PPATPNPSGM AAGDPSTRSW PLGGRAEGGE DEEEVESFPQ
260 270 280 290 300
PVDDYFVEPP QAEEEEEEEE ERAPPPSSHT PVMVSRVTPT PRPTDGVDVY
310 320 330 340 350
FGMPGEIGEH EGFLRAKMDL EERRMRQINE VMREWAMADS QSKNLPKADR
360 370 380 390 400
QALNEHFQSI LQTLEEQVSG ERQRLVETHA TRVIALINDQ RRAALEGFLA
410 420 430 440 450
ALQGDPPQAE RVLMALRRYL RAEQKEQRHT LRHYQHVAAV DPEKAQQMRF
460 470 480 490 500
QVQTHLQVIE ERMNQSLGLL DQNPHLAQEL RPQIQELLLA EHLGPSELDA
510 520 530 540 550
SVPGSSSEDK GSLQPPESKD DPPVTLPKGS TDQESSSSGR EKLTPLEQYE
560 570 580 590 600
QKVNASAPRG FPFHSSDIQR DELAPSGTGV SREALSGLLI MGAGGGSLIV
610 620 630 640 650
LSLLLLRKKK PYGTISHGVV EVDPMLTLEE QQLRELQRHG YENPTYRFLE
653
ERP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3635 Collapse assay details

Data source: Panorama

Official Gene Symbol
Aplp1
Peptide Sequence
LTPLEQYEQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
543
Peptide End
552
CPTAC ID
non-CPTAC-3635
Peptide Molecular Mass
1,247.6398
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 12.8 4.7 3.4 15 5.4 6 19.7 7.2 6.9 15 15 15
b2 (1+) 22.5 4.6 5.8 33.1 5.7 5.2 40 7.3 7.8 15 15 15
y8 (2+) 11.2 3.9 2.7 16.5 6.4 4.9 19.9 7.5 5.6 15 15 15
y6 (1+) 24.2 6 2.5 26.1 6.5 4.1 35.6 8.8 4.8 15 15 15
y7 (1+) 24.4 5 5.9 26.5 3.5 6.1 36 6.1 8.5 15 15 15
sum 8.7 2.7 2.1 15.1 5.2 4.2 17.4 5.9 4.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments