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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pycard Asc
Sequence Length (AA) Molecular Weight (Da)
193 21459
Protein Name
Apoptosis-associated speck-like protein containing a CARD
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGRARDAILD ALENLSGDEL KKFKMKLLTV QLREGYGRIP RGALLQMDAI
60 70 80 90 100
DLTDKLVSYY LESYGLELTM TVLRDMGLQE LAEQLQTTKE ESGAVAAAAS
110 120 130 140 150
VPAQSTARTG HFVDQHRQAL IARVTEVDGV LDALHGSVLT EGQYQAVRAE
160 170 180 190 193
TTSQDKMRKL FSFVPSWNLT CKDSLLQALK EIHPYLVMDL EQS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3636 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pycard
Peptide Sequence
TGHFVDQHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
109
Peptide End
117
CPTAC ID
non-CPTAC-3636
Peptide Molecular Mass
1,095.5210
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 42.7 35.5 23.5 54.5 41.6 32.7 69.2 54.7 40.3 15 15 15
y7 (3+) 10.4 9.9 5.4 11.8 10.1 6.2 15.7 14.1 8.2 15 15 15
y3 (2+) 22.3 12.1 8.5 21.2 16.3 10.1 30.8 20.3 13.2 15 15 15
y2 (1+) 10.6 8.1 11.2 18.3 10.6 11.5 21.1 13.3 16.1 15 15 15
y4 (1+) 10.3 5.7 5.5 9.4 10.1 7.3 13.9 11.6 9.1 15 15 15
sum 6.7 6.1 2.3 6.3 8.3 4.2 9.2 10.3 4.8 15 15 15


Additional Resources and Comments