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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Mug2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mug2 Mug-2
Sequence Length (AA) Molecular Weight (Da)
1451 162382
Protein Name
Murinoglobulin-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWKSRRAQLC LFSVLLAFLP SASSLNGDSK YMVLVPSQLY TETPEKICLH
60 70 80 90 100
LYHLNETVTV TASLVSQTGR RNLFDELVVD KDLFQCVSFI IPTLNSPDEE
110 120 130 140 150
EFLYVDIKGP THEFSKRNAV LVKNKESVVF VQTDKPVYKP GQSVKFRVVS
160 170 180 190 200
MDKTLRPLNE LLPLAYIEDP KKNRIMQWRD IKTENGLKQM SFSLAAEPIQ
210 220 230 240 250
GPYKIVVHKQ SGVKEEHSFT VMEFVLPRFN VDLKVPNAIS VNDEVLQVTV
260 270 280 290 300
CGKYTYGKPV PGQVKISICH ETEAGCKEVN SKLDNNGCST QEVNITELQS
310 320 330 340 350
KKRNYEVQLF HVNATVTEEG TGLEFNGYGT TKIERITNKL IFLKADSHFR
360 370 380 390 400
HGIPFFVKVR LVDIKGDPIP NERVFIKAQV LGYTSATTTD QHGLAKFSID
410 420 430 440 450
TAGFSGSSLH IKVNHKGKDS CYFFYCMEER YASAEHVAYA VYSLSKSYIY
460 470 480 490 500
LVKETSSILP CNQIHTVQAH FILKGDLGVL KELVFYYLVM AQGSIIQTGN
510 520 530 540 550
HTHQVEPGEA PVKGNFDLEI PVEFSMAPMA KMLIYTILPD GEVIADSVNF
560 570 580 590 600
EIEKCLRNKV DLSFSSSQSL PASQTRLQVT ASPQSLCGLR AVDQSVLLLK
610 620 630 640 650
PEDELSPSWI YNLPGMQHNK FIPSSSLSED REDCILYSSW VAEKHTDWVP
660 670 680 690 700
HGREKDVYRY VEDMDLKAFT NLKIKLPKIC FDSAPMSGPR GKFDLAFSSE
710 720 730 740 750
VSGTLQKGSS KRPQPEEPPR EDPPPKDPLA ETIRKYFPET WVWDIVTVNS
760 770 780 790 800
TGVAEVEMTV PDTITEWKAG ALCLSNDTGL GLSSVVPLQA FQPFFVEVSL
810 820 830 840 850
PYSVVRGEAF MLKATVMNYL PTSMRMSVQL EASPDFTAVP VGDDHDSYCL
860 870 880 890 900
SANGRHTSSW LVTPKSLGNV NFSVSVEAQQ SSEPCGSEVA TVPETGRKDT
910 920 930 940 950
VVKVLIVEPE GIKQEHTFNS LFCASDAEIS EKMSLVLPPT VVKDSARAHF
960 970 980 990 1000
SVMGDILSSA IKNTQNLLHM PYGCGEQNMV LFAPNIYVLK YLDKTQQLTQ
1010 1020 1030 1040 1050
KIKTKALGFL RAGYQRELNY KHKDGSYSAF GDQNGEREGN TWLTAFVLKS
1060 1070 1080 1090 1100
FAQARAFIFI DESHITHAFT WLSQQQKDNG CFRSSGSLFH NDIKHPVVSK
1110 1120 1130 1140 1150
ALSCLESSWK TIEQGRNANF VYTKALMAYA FALAGNQDKR NEILKSLDEE
1160 1170 1180 1190 1200
AIKEDNSIHW ERPQKPRKSE HNLYKPQASS VEVEMNAYVV LARLTAQPAP
1210 1220 1230 1240 1250
SPEDLTLSRS TIMWLTKQQN SNGGFSSTQD TVVALDALSK YGAVTFSRRQ
1260 1270 1280 1290 1300
KTSLVTIQST GSFSQKFQVE NSNCLLLQQV PLPDIPGDYT ISVSGEGCVY
1310 1320 1330 1340 1350
AQTTLRYNMH LEKQQSAFAL RVQTVPLTCN NPKGHNSFQI SLEISYTGSR
1360 1370 1380 1390 1400
PASNMVIADV KMLSGFIPLK PTVKKLERLE HISRTEVSNN NVLLYLDQVT
1410 1420 1430 1440 1450
NQTLAFSFII QQDISVRNLQ PAIVKVYDYY ETDEVAYAEY SSPCSSDKQN
1451
V

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3657 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mug2
Peptide Sequence
AQVLGYTSATTTDQHGLAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
378
Peptide End
396
CPTAC ID
non-CPTAC-3657
Peptide Molecular Mass
1,960.9854
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y17 (2+) 6.7 6.9 6.1 11.9 7.8 8.6 13.7 10.4 10.5 15 15 15
b3 (1+) 9.7 2.4 6.6 9.1 4.6 7.5 13.3 5.2 10 15 15 15
b2 (1+) 7.2 6.1 4.7 12.5 6.9 8.5 14.4 9.2 9.7 15 15 15
y15 (2+) 15.5 7.1 6.3 16.2 6.5 6.3 22.4 9.6 8.9 15 15 15
y16 (2+) 14 6.3 7.5 17.2 12.6 7.2 22.2 14.1 10.4 15 15 15
sum 3.7 2.7 1.8 9.8 4.3 5 10.5 5.1 5.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments