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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Mug1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mug1 Mug-1
Sequence Length (AA) Molecular Weight (Da)
1476 165298
Protein Name
Murinoglobulin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWKSRRAQLC LFSVLLAFLH SASLLNGDSK YMVLVPSQLY TETPEKICLH
60 70 80 90 100
LYQLNETVTV TASLVSQSGR KNLFDELVLD KDLFQCVSFI IPRLSSSDEE
110 120 130 140 150
DFLYVDIKGP THEFSKRKAV LVKNKESVVF VQTDKPVYKP GQSVKFRVVS
160 170 180 190 200
MDKMLRPLNE LLPLAYIEDP KKNRIMQWRD IKTENGLKQM SFSLAAEPIQ
210 220 230 240 250
GPYKIVVHKE SGEKEEHSFT VMEFVLPRFN VDLKVPNAMS VNDEVLSVTA
260 270 280 290 300
CGKYTYGKPV PGHVKINVCR ETETGCREVN SQLDNNGCST QEVNITELQS
310 320 330 340 350
KKRNYEVQLF HVNATVTEEG TGLEFSRSGT TKIERITNKL IFLKADSHFR
360 370 380 390 400
HGIPFFVKVR LVDIKGDPIP NEKVFIKAQE LSYTSATTTD QHGLAEFSID
410 420 430 440 450
TTCISGSSLH IKVNHKEEDS CSYFYCMEER HASAKHVAYA VYSLSKSYIY
460 470 480 490 500
LDTETSSILP CNQIHTVQAH FILKGDLGVL KELIFYYLVM AQGSIIQTGN
510 520 530 540 550
HTHQVEPGEA PVKGKFALEI PVEFSMVPMA KMLIYTILPD GEVIADSVNF
560 570 580 590 600
EIEKCLRNKV DLRFSTSQSL PASQTRLQVT ASPQSLCGLR AVDQSVLLLK
610 620 630 640 650
PESELSPSWI YNLPGMQQNK FVPSSRLSED QEDCILYSSW LAEKHTNLVP
660 670 680 690 700
HGTEKDVYRY VEDMGLTAFT NLMIKLPIIC FDYGMVPISA PRVEFDLAFT
710 720 730 740 750
PEISWSLRTT LSKRPEEPPR KDPSSNDPLT ETIRKYFPET WVWDIVTVNS
760 770 780 790 800
TGLAEVEMTV PDTITEWKAG ALCLSNDTGL GLSSVVPLQA FKPFFVEVSL
810 820 830 840 850
PYSVVRGEAF MLKATVMNYL PTSMQMSVQL EASPDFTAVP VGDDQDSYCL
860 870 880 890 900
SANGRHTSSW LVTPKSLGNV NFSVSAEAQQ SSEPCGSEVA TVPETGRKDT
910 920 930 940 950
VVKVLIVEPE GIKQEHTFSS LFCASDAEIS EKMSLVLPPT VVKDSARAHF
960 970 980 990 1000
SVMGDILSSA IRNTQNLLHM PYGCGEQNMV LFAPNIYVLK YLNETQQLTQ
1010 1020 1030 1040 1050
KIKTKALGFL RAGYQRELNY KHKDGSYSAF GDQNGEREGN TWLTAFVLKS
1060 1070 1080 1090 1100
FAQARAFIFI DESHITHAFT WLSQKQKDNG CFRSSGSLFN NAMKGGVDDE
1110 1120 1130 1140 1150
MTLSAYITMA LLESSLPATH PVVSKALSCL ESSWKTIEQE RNASFVYTKA
1160 1170 1180 1190 1200
LMAYAFALAG NQNKRDEILK SLDEEAIKEN NSIHWKRPQK SRKSEHHLYK
1210 1220 1230 1240 1250
PQASSAEVEM NAYVVLARLT AQPAPSPEDL TLSMSTIMWL TKQQNSNGGF
1260 1270 1280 1290 1300
SSTQDTVVAL DALSKYGAVT FSRSQKTTLV TIQSTGSFSQ KFQVENSNRL
1310 1320 1330 1340 1350
LLQQVALPDI PGDYTISVSG EGCVYAQTML RYNMHLEKQL SAFAIWVQTV
1360 1370 1380 1390 1400
PLTCNNPKGH NSFQISLEIS YTGSRPASNM VIADVKMLSG FIPLKPTVKK
1410 1420 1430 1440 1450
LERLEHVSRT EVSNNNVLIY LDQVTNQTLA FSFIIQQDIP VRNLQPAIVK
1460 1470 1476
VYDYYETDEM AFAEYSSPCS TDKQNV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3658 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mug1
Peptide Sequence
HTNLVPHGTEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
645
Peptide End
655
CPTAC ID
non-CPTAC-3658
Peptide Molecular Mass
1,231.6309
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 6.2 5.2 6.6 12.9 6.5 11.4 14.3 8.3 13.2 15 15 15
b3 (1+) 13.2 8.5 5.3 14.7 12.5 5 19.8 15.1 7.3 15 15 15
b2 (1+) 10.6 7.2 10.4 13.9 11.5 17.9 17.5 13.6 20.7 15 15 15
y6 (2+) 10.7 9.6 11.1 16.1 9.3 9.7 19.3 13.4 14.7 15 15 15
y6 (1+) 10.8 8.7 6.7 18.2 9.7 9.8 21.2 13 11.9 15 15 15
sum 3.7 4.3 4.7 13.2 5.6 8 13.7 7.1 9.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments