Nectin1, CPTAC-3660 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Nectin1	Hvec, Prr1, Pvrl1

Sequence Length (AA)	Molecular Weight (Da)
515	57035

Protein Name
Nectin-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARMGLAGAA	GRWWGLALGL	TAFFLPGTHT	QVVQVNDSMY	GFIGTDVVLH
60	70	80	90	100
CSFANPLPSV	KITQVTWQKA	SNGSKQNMAI	YNPTMGVSVL	PPYEKRVEFL
110	120	130	140	150
RPSFIDGTIR	LSGLELEDEG	MYICEFATFP	TGNRESQLNL	TVMAKPTNWI
160	170	180	190	200
EGTRAVLRAR	KGQDDKVLVA	TCTSANGKPP	SAVSWETRLK	GEAEYQEIRN
210	220	230	240	250
PNGTVTVISR	YRLVPSREAH	RQSLACIVNY	HLDRFRESLT	LNVQYEPEVT
260	270	280	290	300
IEGFDGNWYL	QRTDVKLTCK	ADANPPATEY	HWTTLNGSLP	KGVEAQNRTL
310	320	330	340	350
FFRGPITYSL	AGTYICEATN	PIGTRSGQVE	VNITEFPYTP	TPEHGRRAGQ
360	370	380	390	400
MPTAIIGGVA	GSVLLVLIVV	GGIIVALRRR	RHTFKGDYST	KKHVYGNGYS
410	420	430	440	450
KAGIPQHHPP	MAQNLQYPDD	SDDEKKAGPL	GGSSYEEEEE	EEGGGGGERK
460	470	480	490	500
VGGPHPKYDE	DAKRPYFTVD	EAEARQDGYG	DRTLGYQYDP	EQLDLAENMV
510	515
SQNDGSFISK	KEWYV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3660 Collapse assay details

Data source: Panorama

Official Gene Symbol: Nectin1
Peptide Sequence: RPYFTVDEAEAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 464
Peptide End: 475

CPTAC ID: non-CPTAC-3660
Peptide Molecular Mass: 1,452.6997
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b5 (1+)	13.5	4.9	8.8	13.3	6.3	9	19	8	12.6	15	15	15
y2 (1+)	10.4	6.9	10.8	16.8	11.6	10.2	19.8	13.5	14.9	15	15	15
y3 (1+)	10.1	8.4	3.4	16.4	8.4	4.2	19.3	11.9	5.4	15	15	15
y4 (1+)	9.8	5.3	4.7	6.6	5	7.5	11.8	7.3	8.9	15	15	15
y6 (1+)	16	5.3	5.1	17.3	6.7	7	23.6	8.5	8.7	15	15	15
sum	4.9	2.3	2.5	6.7	3.2	3.9	8.3	3.9	4.6	15	15	15

Nectin1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3660 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Nectin1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3660 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments