Nrp1, CPTAC-3661 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Nrp1	Nrp

Sequence Length (AA)	Molecular Weight (Da)
923	103000

Protein Name
Neuropilin-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MERGLPLLCA	TLALALALAG	AFRSDKCGGT	IKIENPGYLT	SPGYPHSYHP
60	70	80	90	100
SEKCEWLIQA	PEPYQRIMIN	FNPHFDLEDR	DCKYDYVEVI	DGENEGGRLW
110	120	130	140	150
GKFCGKIAPS	PVVSSGPFLF	IKFVSDYETH	GAGFSIRYEI	FKRGPECSQN
160	170	180	190	200
YTAPTGVIKS	PGFPEKYPNS	LECTYIIFAP	KMSEIILEFE	SFDLEQDSNP
210	220	230	240	250
PGGMFCRYDR	LEIWDGFPEV	GPHIGRYCGQ	KTPGRIRSSS	GVLSMVFYTD
260	270	280	290	300
SAIAKEGFSA	NYSVLQSSIS	EDFKCMEALG	MESGEIHSDQ	ITASSQYGTN
310	320	330	340	350
WSVERSRLNY	PENGWTPGED	SYKEWIQVDL	GLLRFVTAVG	TQGAISKETK
360	370	380	390	400
KKYYVKTYRV	DISSNGEDWI	SLKEGNKAII	FQGNTNPTDV	VLGVFSKPLI
410	420	430	440	450
TRFVRIKPVS	WETGISMRFE	VYGCKITDYP	CSGMLGMVSG	LISDSQITAS
460	470	480	490	500
NQADRNWMPE	NIRLVTSRTG	WALPPSPHPY	TNEWLQVDLG	DEKIVRGVII
510	520	530	540	550
QGGKHRENKV	FMRKFKIAYS	NNGSDWKTIM	DDSKRKAKSF	EGNNNYDTPE
560	570	580	590	600
LRTFSPLSTR	FIRIYPERAT	HSGLGLRMEL	LGCEVEAPTA	GPTTPNGNPV
610	620	630	640	650
DECDDDQANC	HSGTGDDFQL	TGGTTVLATE	KPTIIDSTIQ	SEFPTYGFNC
660	670	680	690	700
EFGWGSHKTF	CHWEHDSHAQ	LRWSVLTSKT	GPIQDHTGDG	NFIYSQADEN
710	720	730	740	750
QKGKVARLVS	PVVYSQSSAH	CMTFWYHMSG	SHVGTLRVKL	RYQKPEEYDQ
760	770	780	790	800
LVWMVVGHQG	DHWKEGRVLL	HKSLKLYQVI	FEGEIGKGNL	GGIAVDDISI
810	820	830	840	850
NNHISQEDCA	KPTDLDKKNT	EIKIDETGST	PGYEGEGEGD	KNISRKPGNV
860	870	880	890	900
LKTLDPILIT	IIAMSALGVL	LGAVCGVVLY	CACWHNGMSE	RNLSALENYN
910	920	923
FELVDGVKLK	KDKLNPQSNY	SEA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3661 Collapse assay details

Data source: Panorama

Official Gene Symbol: Nrp1
Peptide Sequence: IAPSPVVSSGPFLFIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 107
Peptide End: 122

CPTAC ID: non-CPTAC-3661
Peptide Molecular Mass: 1,657.9443
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	10	13	9.6	19.1	15	14.4	21.6	19.8	17.3	15	15	15
y14 (2+)	6	5.8	4.1	15.7	9.8	7.4	16.8	11.4	8.5	15	15	15
y15 (2+)	23.9	25.9	14.1	35.6	33.1	23.7	42.9	42	27.6	15	15	15
y9 (1+)	11.4	12.2	7.9	19.6	13.4	10.6	22.7	18.1	13.2	15	15	15
y10 (1+)	28.3	23.9	15.4	34.5	27.9	21.8	44.6	36.7	26.7	15	15	15
sum	3.9	6.5	4	14.2	8.9	6.9	14.7	11	8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3597 Collapse assay details

Data source: Panorama

Official Gene Symbol: Nrp1
Peptide Sequence: SFEGNNNYDTPELR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 539
Peptide End: 552

CPTAC ID: non-CPTAC-3597
Peptide Molecular Mass: 1,654.7223
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.