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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Nrp1 Nrp
Sequence Length (AA) Molecular Weight (Da)
923 103000
Protein Name
Neuropilin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP
60 70 80 90 100
SEKCEWLIQA PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW
110 120 130 140 150
GKFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN
160 170 180 190 200
YTAPTGVIKS PGFPEKYPNS LECTYIIFAP KMSEIILEFE SFDLEQDSNP
210 220 230 240 250
PGGMFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS GVLSMVFYTD
260 270 280 290 300
SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN
310 320 330 340 350
WSVERSRLNY PENGWTPGED SYKEWIQVDL GLLRFVTAVG TQGAISKETK
360 370 380 390 400
KKYYVKTYRV DISSNGEDWI SLKEGNKAII FQGNTNPTDV VLGVFSKPLI
410 420 430 440 450
TRFVRIKPVS WETGISMRFE VYGCKITDYP CSGMLGMVSG LISDSQITAS
460 470 480 490 500
NQADRNWMPE NIRLVTSRTG WALPPSPHPY TNEWLQVDLG DEKIVRGVII
510 520 530 540 550
QGGKHRENKV FMRKFKIAYS NNGSDWKTIM DDSKRKAKSF EGNNNYDTPE
560 570 580 590 600
LRTFSPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEAPTA GPTTPNGNPV
610 620 630 640 650
DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC
660 670 680 690 700
EFGWGSHKTF CHWEHDSHAQ LRWSVLTSKT GPIQDHTGDG NFIYSQADEN
710 720 730 740 750
QKGKVARLVS PVVYSQSSAH CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ
760 770 780 790 800
LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI FEGEIGKGNL GGIAVDDISI
810 820 830 840 850
NNHISQEDCA KPTDLDKKNT EIKIDETGST PGYEGEGEGD KNISRKPGNV
860 870 880 890 900
LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN
910 920 923
FELVDGVKLK KDKLNPQSNY SEA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3661 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nrp1
Peptide Sequence
IAPSPVVSSGPFLFIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
107
Peptide End
122
CPTAC ID
non-CPTAC-3661
Peptide Molecular Mass
1,657.9443
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 10 13 9.6 19.1 15 14.4 21.6 19.8 17.3 15 15 15
y14 (2+) 6 5.8 4.1 15.7 9.8 7.4 16.8 11.4 8.5 15 15 15
y15 (2+) 23.9 25.9 14.1 35.6 33.1 23.7 42.9 42 27.6 15 15 15
y9 (1+) 11.4 12.2 7.9 19.6 13.4 10.6 22.7 18.1 13.2 15 15 15
y10 (1+) 28.3 23.9 15.4 34.5 27.9 21.8 44.6 36.7 26.7 15 15 15
sum 3.9 6.5 4 14.2 8.9 6.9 14.7 11 8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3597 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nrp1
Peptide Sequence
SFEGNNNYDTPELR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
539
Peptide End
552
CPTAC ID
non-CPTAC-3597
Peptide Molecular Mass
1,654.7223
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 10.7 11.8 9.9 12.7 10.9 9.1 16.6 16.1 13.4 15 15 15
y2 (1+) 9.4 11.2 13 10.6 15 15.2 14.2 18.7 20 15 15 15
y4 (1+) 10.3 9.1 5 12.6 11 10.4 16.3 14.3 11.5 15 15 15
y5 (1+) 14.9 5.2 9.9 18 6.7 9.7 23.4 8.5 13.9 15 15 15
y6 (1+) 11.4 10.9 9 13.9 9.2 12 18 14.3 15 15 15 15
sum 5.7 5.2 5.2 8.3 6.8 7.6 10.1 8.6 9.2 15 15 15


Additional Resources and Comments