Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Nucb1

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Nucb1 Nuc, Nucb
Sequence Length (AA) Molecular Weight (Da)
459 53409
Protein Name
Nucleobindin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPTSVPRGAP FLLLPPLLML SAVLAVPVDR AAPPQEDSQA TETPDTGLYY
60 70 80 90 100
HRYLQEVINV LETDGHFREK LQAANAEDIK SGKLSQELDF VSHNVRTKLD
110 120 130 140 150
ELKRQEVSRL RMLLKAKMDA KQEPNLQVDH MNLLKQFEHL DPQNQHTFEA
160 170 180 190 200
RDLELLIQTA TRDLAQYDAA HHEEFKRYEM LKEHERRRYL ESLGEEQRKE
210 220 230 240 250
AERKLQEQQR RHREHPKVNV PGSQAQLKEV WEELDGLDPN RFNPKTFFIL
260 270 280 290 300
HDINSDGVLD EQELEALFTK ELEKVYDPKN EEDDMREMEE ERLRMREHVM
310 320 330 340 350
KNVDTNQDRL VTLEEFLAST QRKEFGDTGE GWKTVEMSPA YTEEELKRFE
360 370 380 390 400
EELAAREAEL NARAQRLSQE TEALGRSQDR LEAQKRELQQ AVLQMEQRKQ
410 420 430 440 450
QLQEQSAPPS KPDGQLQFRA DTDDAPVPAP AGDQKDVPAS EKKVPEQPPE
459
LPQLDSQHL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3662 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nucb1
Peptide Sequence
VNVPGSQAQLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
218
Peptide End
228
CPTAC ID
non-CPTAC-3662
Peptide Molecular Mass
1,139.6299
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 36.5 11.3 7 40.8 17.2 6.7 54.7 20.6 9.7 15 15 15
y8 (1+) 10.7 5.7 5.8 15.3 6.2 8 18.7 8.4 9.9 15 15 15
b2 (1+) 10.7 4.5 4.7 12.5 6.1 7.3 16.5 7.6 8.7 15 15 15
y8 (2+) 23.3 8.4 7.5 23.5 11.2 8.3 33.1 14 11.2 15 15 15
b3 (1+) 10.8 5 4.4 17.9 6.7 7.5 20.9 8.4 8.7 15 15 15
sum 6.5 2.9 2.3 9.2 5.2 5.8 11.3 6 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments