Cryz, CPTAC-3666 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Cryz	N/A

Sequence Length (AA)	Molecular Weight (Da)
331	35269

Protein Name
Quinone oxidoreductase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATGQKLMRA	IRVFEFGGPE	VLKLQSDVVV	PVPQSHQVLI	KVHACGVNPV
60	70	80	90	100
ETYIRSGAYS	RKPALPYTPG	SDVAGIIESV	GDKVSAFKKG	DRVFCYSTVS
110	120	130	140	150
GGYAEFALAA	DDTIYPLPET	LNFRQGAALG	IPYFTACRAL	FHSARARAGE
160	170	180	190	200
SVLVHGASGG	VGLATCQIAR	AHGLKVLGTA	GSEEGKKLVL	QNGAHEVFNH
210	220	230	240	250
KEANYIDKIK	MSVGDKDKGV	DVIIEMLANE	NLSNDLKLLS	HGGRVVVVGC
260	270	280	290	300
RGPIEINPRD	TMAKETSIIG	VSLSSSTKEE	FQQFAGLLQA	GIEKGWVKPV
310	320	330	331
IGSEYPLEKA	AQAHEDIIHG	SGKTGKMILL	L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3666 Collapse assay details

Data source: Panorama

Official Gene Symbol: Cryz
Peptide Sequence: AAQAHEDIIHGSGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 310
Peptide End: 323

CPTAC ID: non-CPTAC-3666
Peptide Molecular Mass: 1,432.7059
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y13 (3+)	13.2	12.5	12.2	13	14.2	15.5	18.5	18.9	19.7	15	15	15
y10 (2+)	11.3	8.4	8.9	11.2	9.5	9.6	15.9	12.7	13.1	15	15	15
y11 (2+)	6.2	7.5	11.6	9.7	6.5	12.9	11.5	9.9	17.3	15	15	15
y4 (1+)	5	11.4	8	8.7	9.5	10.3	10	14.8	13	15	15	15
y5 (1+)	8.1	8.9	14.2	10.3	8.2	13.2	13.1	12.1	19.4	15	15	15
sum	4.4	5	5.5	7	5.5	5.5	8.3	7.4	7.8	15	15	15

Cryz

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3666 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Cryz

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3666 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments