Stip1, CPTAC-3673 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Stip1	N/A

Sequence Length (AA)	Molecular Weight (Da)
543	62582

Protein Name
Stress-induced-phosphoprotein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEQVNELKEK	GNKALSAGNI	DDALQCYSEA	IKLDPQNHVL	YSNRSAAYAK
60	70	80	90	100
KGDYQKAYED	GCKTVDLKPD	WGKGYSRKAA	ALEFLNRFEE	AKRTYEEGLK
110	120	130	140	150
HEANNLQLKE	GLQNMEARLA	ERKFMNPFNL	PNLYQKLEND	PRTRSLLSDP
160	170	180	190	200
TYRELIEQLQ	NKPSDLGTKL	QDPRVMTTLS	VLLGVDLGSM	DEEEEAATPP
210	220	230	240	250
PPPPPKKEPK	PEPMEEDLPE	NKKQALKEKE	LGNDAYKKKD	FDKALKHYDR
260	270	280	290	300
AKELDPTNMT	YITNQAAVHF	EKGDYNKCRE	LCEKAIEVGR	ENREDYRQIA
310	320	330	340	350
KAYARIGNSY	FKEEKYKDAI	HFYNKSLAEH	RTPDVLKKCQ	QAEKILKEQE
360	370	380	390	400
RLAYINPDLA	LEEKNKGNEC	FQKGDYPQAM	KHYTEAIKRN	PRDAKLYSNR
410	420	430	440	450
AACYTKLLEF	QLALKDCEEC	IQLEPTFIKG	YTRKAAALEA	MKDYTKAMDV
460	470	480	490	500
YQKALDLDSS	CKEAADGYQR	CMMAQYNRHD	SPEDVKRRAM	ADPEVQQIMS
510	520	530	540	543
DPAMRLILEQ	MQKDPQALSE	HLKNPVIAQK	IQKLMDVGLI	AIR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3673 Collapse assay details

Data source: Panorama

Official Gene Symbol: Stip1
Peptide Sequence: HEANNLQLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 101
Peptide End: 109

CPTAC ID: non-CPTAC-3673
Peptide Molecular Mass: 1,065.5567
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b5 (1+)	8.2	7.2	8.2	8.7	7.2	13.1	12	10.2	15.5	15	15	15
b4 (1+)	13	12.3	15.8	10.8	10.7	17.4	16.9	16.3	23.5	15	15	15
b2 (1+)	14.5	7.8	11.5	14.1	8.1	9.7	20.2	11.2	15	15	15	15
y2 (1+)	9.5	5.2	6.2	9.8	6.8	13.7	13.6	8.6	15	15	15	15
y3 (1+)	6.9	6.4	7.3	7.4	6.6	9	10.1	9.2	11.6	15	15	15
sum	5	3.3	5.5	5.4	4.2	7.2	7.4	5.3	9.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3674 Collapse assay details

Data source: Panorama

Official Gene Symbol: Stip1
Peptide Sequence: LDPQNHVLYSNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 33
Peptide End: 44

CPTAC ID: non-CPTAC-3674
Peptide Molecular Mass: 1,454.7266
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers