Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Sh3bgrl N/A
Sequence Length (AA) Molecular Weight (Da)
114 12811
Protein Name
SH3 domain-binding glutamic acid-rich-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVIRVYIASS SGSTAIKKKQ QDVLCFLEAN KIGFEEKDIA ANEENRKWMR
60 70 80 90 100
ENVPEDSRPS TGYPLPPQIF NECQYRGDYD AFFEARENNA VYAFLGLTAP
110 114
PGSKEAEAQA NQQA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-585 Collapse assay details

Data source: Panorama

Official Gene Symbol
SH3BGRL
Peptide Sequence
ENNAVYAFLGLTAPPGSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
87
Peptide End
104
CPTAC ID
CPTAC-585
Peptide Molecular Mass
1,847.9418
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 14.2 11.8 5.9 20.5 17 18.7 24.9 20.7 19.6 25 15 15
y10 (1+) 23.2 14.5 7.5 33.2 16.1 17 40.5 21.7 18.6 25 15 15
y5 (1+) 10 6.7 4.8 16.7 10 17.1 19.5 12 17.8 25 15 15
sum 9.1 6.6 4.1 17.1 10.1 16.7 19.4 12.1 17.2 25 15 15


Additional Resources and Comments


Assay Details for CPTAC-584 Collapse assay details

Data source: Panorama

Official Gene Symbol
SH3BGRL
Peptide Sequence
GDYDAFFEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
77
Peptide End
86
CPTAC ID
CPTAC-584
Peptide Molecular Mass
1,189.5040
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 12.2 8 4.7 15.5 13.5 7.7 19.7 15.7 9 25 15 15
y7 (1+) 7.8 4.9 5.2 10.7 7.5 8.3 13.2 9 9.8 25 15 15
y6 (1+) 6.4 3.7 4.1 9.9 6.5 6 11.8 7.5 7.3 25 15 15
sum 6.3 4.2 4 10 7.2 6.7 11.8 8.3 7.8 25 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3672 Collapse assay details

Data source: Panorama

Official Gene Symbol
Sh3bgrl
Peptide Sequence
GDYDAFFEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
77
Peptide End
86
CPTAC ID
non-CPTAC-3672
Peptide Molecular Mass
1,189.5040
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 13.9 12 5.1 21 13.8 8.9 25.2 18.3 10.3 15 15 15
y4 (1+) 15.2 7.8 4.9 23.3 10 5.6 27.8 12.7 7.4 15 15 15
y5 (1+) 7.6 2.6 5.7 11.5 5.3 5.9 13.8 5.9 8.2 15 15 15
y6 (1+) 7.4 6.6 6 10.7 5.8 6.4 13 8.8 8.8 15 15 15
y7 (1+) 11 5.5 5.3 18.5 8.7 6.5 21.5 10.3 8.4 15 15 15
sum 4.6 3.5 2.1 12.7 4.8 4.2 13.5 5.9 4.7 15 15 15


Additional Resources and Comments