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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Adgrg6 Dreg, Gm222, Gpr126
Sequence Length (AA) Molecular Weight (Da)
1165 129721
Protein Name
Adhesion G-protein coupled receptor G6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMFDTLGKRC CPWRLKPSAL LFLFVLCVTC VPLSVCGCGS CRLVLSNPSG
60 70 80 90 100
TFTSPCYPND YPNTQSCSWT LRAPAGYIIQ ITFNDFDIEE APNCIYDSLS
110 120 130 140 150
LDNGESQTKF CGATAKGLSF NSSVNEMHVS FSSDFSIQKK GFNASYIRVA
160 170 180 190 200
VSLRNQKVIL PQTLDAYQVS VAKSISIPEL KAFTLCFEAS KVGNEGGDWT
210 220 230 240 250
AFSYSDESLT QLLSLEKASN GYFLSISGSR CLLNNALPVK DKEDIFTENL
260 270 280 290 300
EQLCLVWNNS WGSIGINFKK NYETVPCDST ISAVVPGDGT LLLGSDRDEV
310 320 330 340 350
ASLRGSIYNF RLWNFTMDLK ALSNLSCSVS GNVIDWHNDF WSISTQALKA
360 370 380 390 400
EGNLSCGSYL IQLPAAELTN CSELGTLCQD GIMYRISVVI HNDFNHPEVK
410 420 430 440 450
VQTKVAEWLN STFQNWNYTV YVVNISFHQK VGEDRMKVKR DIMDDDKRLV
460 470 480 490 500
LWALLVYNAT NNVSLNEEKI KQKLMTNNAS LEDGLRLCEV DVNQLGMCSA
510 520 530 540 550
LEDPDGFSWP ATLPSVYKQP CPNKPGFFMT RACLSNGTST FWGPVDTSNC
560 570 580 590 600
SRQSNEVANE ILNQTGDGQN LTSANINSIV EKVKRIVNKE ENIDITLGST
610 620 630 640 650
LMNIFSNILS SSDSDLLESS TEALKTIDEL AFKIDLNSTP HVNIETQNLA
660 670 680 690 700
LGVSSLIPGT NAPSNFSIGL PSNNESYFQM DFGNGQTDPL ASVILPPNLL
710 720 730 740 750
ENLSPEDSVL VRRAQFTFFN KTGLFQDVGS QRKVLVSYVM ACSIGNITIQ
760 770 780 790 800
NLKDPVQIKI KHTRTQEVHH PICAFWDMNK NKSFGGWNTS GCVAHSDLDA
810 820 830 840 850
GETICLCSHF THFGVLMDLP RSASQIDGRN TKVLTFITYI GCGISAIFSA
860 870 880 890 900
ATLLTYVAFE KLRRDYPSKI LMNLSSALLF LNLIFLLDGW VTSFGVAGLC
910 920 930 940 950
TAVAALLHFF LLATFTWMGL EAIHMYIALV KVFNTYIHRY ILKFCIIGWG
960 970 980 990 1000
LPALVVSIIL VSRRQNEVYG KESYGKDQDD EFCWIQDPVV FYVSCAGYFG
1010 1020 1030 1040 1050
VMFFLNVAMF IVVMVQICGR NGKRSNRTLR EEVLRNLRSV VSLTFLLGMT
1060 1070 1080 1090 1100
WGFAFFAWGP LNIPFMYLFS IFNSLQGLFI FIFHCAMKEN VQKQWRRHLC
1110 1120 1130 1140 1150
CGRFRLADNS DWSKTATNII KKSSDNLGKS LSSSSIGSNS TYLTSKSKSS
1160 1165
STTYFKRNSH SDNFS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3685 Collapse assay details

Data source: Panorama

Official Gene Symbol
Adgrg6
Peptide Sequence
TIDELAFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
626
Peptide End
633
CPTAC ID
non-CPTAC-3685
Peptide Molecular Mass
935.4964
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 25.4 7.1 5.8 24.6 9.3 9.8 35.4 11.7 11.4 15 15 15
y2 (1+) 24.7 5.7 4 29.2 6.1 8.9 38.2 8.3 9.8 15 15 15
y3 (1+) 61.4 9.7 8.7 60.3 12 9.7 86.1 15.4 13 15 15 15
y4 (1+) 32.4 6.6 7.5 36.5 9.1 7.3 48.8 11.2 10.5 15 15 15
y6 (1+) 7.8 2.9 3.4 14.1 7.8 10.3 16.1 8.3 10.8 15 15 15
sum 5.8 2.5 2.5 12 7.6 8.9 13.3 8 9.2 15 15 15


Additional Resources and Comments