Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Adgrl4 Eltd1
Sequence Length (AA) Molecular Weight (Da)
739 82246
Protein Name
Adhesion G protein-coupled receptor L4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLLPLLVGF STLLNCSYTQ NCSKTTCLPN AKCEVHNGVE ACFCSQGYSG
60 70 80 90 100
NGVTICEDID ECSESSVCGD HAVCENVNGG FSCFCREGYQ TATGKSQFTP
110 120 130 140 150
NDGSYCQDID ECSESSVCGD HAVCENVNGG FSCFCREGYQ TATGKSQFTP
160 170 180 190 200
NDGSYCQESM NSNCHLEHAC IAANINKTLK RIGPITEQTT LLQEIYRNSE
210 220 230 240 250
AELSLMDIVT YIEILTESSS LLGHPNSTTS YKDAHFNSTL TEFGETINNF
260 270 280 290 300
VERSTHKMWD QLPTNHRRLH LTKLMHTAEL VTLQIAQNTQ KNSQFDMNST
310 320 330 340 350
DLALKVFAFD STHMKHAHPH MNVDGGYVKI SPRRKAAHGT TGNVVVAFLC
360 370 380 390 400
YKSIGPLLSS SDNFLLDTQN DNSEGKEKVI SSVISASISS NPPTLYELEK
410 420 430 440 450
ITFTLSHVKL SDKHRTQCAF WNYSVDAMNN GSWSTEGCEL THSNDTHTSC
460 470 480 490 500
RCSHLTHFAI LMSSTSSIGI KDYNILTRIT QLGIIISLIC LAICIFTFWF
510 520 530 540 550
FSEIQSTRTT IHKNLCCSLF LAELVFLIGI NINTNKLVCS IIAGLLHYFF
560 570 580 590 600
LAAFAWMCIE GIHLYLIVVG VIYNKGFLHK NFYIFGYLSP AVVVGFSASL
610 620 630 640 650
GYRYYGTTKV CWLSTENNFI WSFIGPACLI ILVNLLAFGV IIYKVFRHTA
660 670 680 690 700
GLKPEVSCYE NIRSCARGAL ALLFLLGTTW IFGVLHVVHA SVVTAYLFTV
710 720 730 739
SNAFQGMFIF LFLCVLSRKI QEEYYRLFKN VPCCFGCLR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3686 Collapse assay details

Data source: Panorama

Official Gene Symbol
Adgrl4
Peptide Sequence
ITFTLSHVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
401
Peptide End
409
CPTAC ID
non-CPTAC-3686
Peptide Molecular Mass
1,044.5968
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.6 3.4 3 12.2 4 3.7 13.4 5.2 4.8 15 15 15
b2 (1+) 6.6 2.9 2.2 13.1 5 3.5 14.7 5.8 4.1 15 15 15
y7 (2+) 6.2 3 2.2 9.5 4.2 2.3 11.3 5.2 3.2 15 15 15
y8 (2+) 4.8 4.2 1.5 8 4.8 5 9.3 6.4 5.2 15 15 15
y5 (1+) 4.4 2.7 3.5 10.6 4.4 3.1 11.5 5.2 4.7 15 15 15
sum 2.9 1.2 1 8.6 3.1 2.6 9.1 3.3 2.8 15 15 15


Additional Resources and Comments