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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Anxa5 Anx5
Sequence Length (AA) Molecular Weight (Da)
319 35752
Protein Name
Annexin A5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATRGTVTDF PGFDGRADAE VLRKAMKGLG TDEDSILNLL TSRSNAQRQE
60 70 80 90 100
IAQEFKTLFG RDLVDDLKSE LTGKFEKLIV AMMKPSRLYD AYELKHALKG
110 120 130 140 150
AGTDEKVLTE IIASRTPEEL SAIKQVYEEE YGSNLEDDVV GDTSGYYQRM
160 170 180 190 200
LVVLLQANRD PDTAIDDAQV ELDAQALFQA GELKWGTDEE KFITIFGTRS
210 220 230 240 250
VSHLRRVFDK YMTISGFQIE ETIDRETSGN LEQLLLAVVK SIRSIPAYLA
260 270 280 290 300
ETLYYAMKGA GTDDHTLIRV VVSRSEIDLF NIRKEFRKNF ATSLYSMIKG
310 319
DTSGDYKKAL LLLCGGEDD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4015 Collapse assay details

Data source: Panorama

Official Gene Symbol
Anxa5
Peptide Sequence
ETSGNLEQLLLAVVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
226
Peptide End
240
CPTAC ID
non-CPTAC-4015
Peptide Molecular Mass
1,612.9036
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 7.6 3.3 1.2 9.2 5.4 4.6 11.9 6.3 4.8 15 15 15
y9 (1+) 7.9 2.4 2.6 13.3 6.3 4.9 15.5 6.7 5.5 15 15 15
y8 (1+) 18.9 2.6 3.3 19 6 3.7 26.8 6.5 5 15 15 15
y4 (1+) 11.1 5.1 3.6 14.2 8.1 4.5 18 9.6 5.8 15 15 15
y3 (1+) 6.1 2.9 2.3 12.7 3.7 4.9 14.1 4.7 5.4 15 15 15
sum 6.3 1.8 1.4 10.2 5.3 4 12 5.6 4.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3687 Collapse assay details

Data source: Panorama

Official Gene Symbol
Anxa5
Peptide Sequence
GLGTDEDSILNLLTSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
28
Peptide End
43
CPTAC ID
non-CPTAC-3687
Peptide Molecular Mass
1,702.8737
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 18.2 9.5 10.5 21.8 11.9 11.8 28.4 15.2 15.8 15 15 15
y2 (1+) 20.1 6.2 5.8 22.1 6.9 6.2 29.9 9.3 8.5 15 15 15
y3 (1+) 7.3 4.8 5.3 12.3 4.6 5.4 14.3 6.6 7.6 15 15 15
y4 (1+) 11.1 6.4 2.9 14.2 7.1 4 18 9.6 4.9 15 15 15
y6 (1+) 15.2 6.2 6.6 18.1 8 8.8 23.6 10.1 11 15 15 15
sum 7.2 2.4 2.9 10.2 4.3 4.1 12.5 4.9 5 15 15 15


Additional Resources and Comments