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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Lap3 Lapep
Sequence Length (AA) Molecular Weight (Da)
519 56141
Protein Name
Cytosol aminopeptidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MYLLPLPAAA RVALRRLGVR GLWDRGLSTA DMTKGLVLGI YAKDKDDDLP
60 70 80 90 100
QFTSAGESFN KLVSGKLREM LNISGPPLKA GKTRTFYGLH QDFPSVVVVG
110 120 130 140 150
LGKRSAGVDD QENWHEGKEN IRAAVAAGCR QVQDLELPSV EVDPCGDAQA
160 170 180 190 200
AAEGAVLGLY EYDDLKQKKK VAVSAKLHGS GDLEAWEKGV LFASGQNLAR
210 220 230 240 250
HLMESPANEM TPTRFAEIIE KNLKSASSKT KVHIRPKSWI EEQEMGSFLS
260 270 280 290 300
VAKGSEEPPV FLEIHYMGSP NATEAPLVFV GKGITFDSGG ISIKASANMD
310 320 330 340 350
LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV
360 370 380 390 400
RARNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVIINAA TLTGAMDVAL
410 420 430 440 450
GSGATGVFTN SSWLWNKLFE ASVETGDRVW RMPLFEHYTR QVIDCQLADV
460 470 480 490 500
NNLGKYRSAG ACTAAAFLRE FVTHTKWAHL DIAGVMTNKD EIPYLRKGMS
510 519
GRPTRTLIEF LLRFSKDSS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3688 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lap3
Peptide Sequence
GVLFASGQNLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
189
Peptide End
200
CPTAC ID
non-CPTAC-3688
Peptide Molecular Mass
1,231.6673
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 17.6 9.7 7.1 12.9 9.5 10.7 21.8 13.6 12.8 15 15 15
y8 (1+) 11.1 6.3 7.2 14.4 8.1 7.6 18.2 10.3 10.5 15 15 15
y9 (1+) 11.8 5.1 7.3 11.3 8.5 6.2 16.3 9.9 9.6 15 15 15
y10 (2+) 24.7 8.7 7.3 26.6 8.9 8.7 36.3 12.4 11.4 15 15 15
y6 (1+) 10.1 8.6 5.5 9.5 10.1 6.5 13.9 13.3 8.5 15 15 15
sum 6.1 5.5 3 5.2 5.2 4.2 8 7.6 5.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3689 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lap3
Peptide Sequence
TIQVDNTDAEGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
357
Peptide End
368
CPTAC ID
non-CPTAC-3689
Peptide Molecular Mass
1,317.6161
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 17 6 7 18.7 7.7 7.2 25.3 9.8 10 15 15 15
y9 (1+) 10.3 6.6 4.9 11.5 7.6 5.6 15.4 10.1 7.4 15 15 15
b3 (1+) 13.3 7.3 5.1 15 9.5 7.8 20 12 9.3 15 15 15
b2 (1+) 12.4 5.9 5.9 20 6.1 4.7 23.5 8.5 7.5 15 15 15
y4 (1+) 19.3 9 7.1 21.4 8 8.5 28.8 12 11.1 15 15 15
sum 5.4 3.9 3.1 10.4 5 4.3 11.7 6.3 5.3 15 15 15


Additional Resources and Comments