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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Tgfbi

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tgfbi N/A
Sequence Length (AA) Molecular Weight (Da)
683 74597
Protein Name
Transforming growth factor-beta-induced protein ig-h3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALLMRLLTL ALALSVGPAG TLAGPAKSPY QLVLQHSRLR GRQHGPNVCA
60 70 80 90 100
VQKVIGTNKK YFTNCKQWYQ RKICGKSTVI SYECCPGYEK VPGEKGCPAA
110 120 130 140 150
LPLSNLYETM GVVGSTTTQL YTDRTEKLRP EMEGPGSFTI FAPSNEAWSS
160 170 180 190 200
LPAEVLDSLV SNVNIELLNA LRYHMVDRRV LTDELKHGMT LTSMYQNSNI
210 220 230 240 250
QIHHYPNGIV TVNCARLLKA DHHATNGVVH LIDKVISTIT NNIQQIIEIE
260 270 280 290 300
DTFETLRAAV AASGLNTVLE GDGQFTLLAP TNEAFEKIPA ETLNRILGDP
310 320 330 340 350
EALRDLLNNH ILKSAMCAEA IVAGMSMETL GGTTLEVGCS GDKLTINGKA
360 370 380 390 400
VISNKDILAT NGVIHFIDEL LIPDSAKTLL ELAGESDVST AIDILKQAGL
410 420 430 440 450
DTHLSGKEQL TFLAPLNSVF KDGVPRIDAQ MKTLLLNHMV KEQLASKYLY
460 470 480 490 500
SGQTLDTLGG KKLRVFVYRN SLCIENSCIA AHDKRGRFGT LFTMDRMLTP
510 520 530 540 550
PMGTVMDVLK GDNRFSMLVA AIQSAGLMEI LNREGVYTVF APTNEAFQAM
560 570 580 590 600
PPEELNKLLA NAKELTNILK YHIGDEILVS GGIGALVRLK SLQGDKLEVS
610 620 630 640 650
SKNNVVSVNK EPVAETDIMA TNGVVYAINT VLQPPANRPQ ERGDELADSA
660 670 680 683
LEIFKQASAY SRAAQRSVRL APVYQRLLER MKH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3690 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tgfbi
Peptide Sequence
ADHHATNGVVHLIDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
220
Peptide End
234
CPTAC ID
non-CPTAC-3690
Peptide Molecular Mass
1,625.8274
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 10.3 4.5 5.9 11.6 6.2 4.3 15.5 7.7 7.3 15 15 15
y4 (1+) 12.6 5.2 5.1 14.7 5.1 5.6 19.4 7.3 7.6 15 15 15
y13 (3+) 6.3 6 4.9 7.1 7.3 6.4 9.5 9.4 8.1 15 15 15
y14 (3+) 8.5 7 4.5 11.3 7.4 9.3 14.1 10.2 10.3 15 15 15
y5 (2+) 13.6 5.7 5.4 18.3 5 6.3 22.8 7.6 8.3 15 15 15
sum 4.4 3.1 2.4 8.9 3.7 5 9.9 4.8 5.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments