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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Adamts2 N/A
Sequence Length (AA) Molecular Weight (Da)
1213 135299
Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDPPAGAARR LLCPALLLLL LPPPPLLLLP PPPASVRLVA ATEPPGGPPG
60 70 80 90 100
QGAERILAVP VRTDAQGRLV SHVVSLETAG AGVRARRAAL DQTSGLPGGA
110 120 130 140 150
AQDPGGRLFY NLTVFGRDLH LRLRPNARLV APGATVEWQG ETGDTRVEPL
160 170 180 190 200
LGSCLYVGDV ADLPKASSVA LSNCDGLAGL IRMEEEEFFI EPLEKGQTDQ
210 220 230 240 250
EAEQGRVHVV YRRPPTPKPP PVSEPQALDT GVSQGNLDSL SRALGVLEER
260 270 280 290 300
INSSRRRVRR HATDDDYNIE VLLGVDDSVV QFHGKEHVQK YLLTLMNIVN
310 320 330 340 350
EIYHDESLGA HINVVLVRII LLSHAKSMSL IEIGNPSQSL ENVCRWAYLQ
360 370 380 390 400
QKPDTDHDEY HDHAIFLTRQ DFGPSGMQGY APVTGMCHPV RSCTLNHEDG
410 420 430 440 450
FSSAFVVAHE TGHVLGMEHD GQGNRCGDEV RLGSIMAPLV QAAFHRFHWS
460 470 480 490 500
RCSQQELSRY LHSYDCLRDD PFAHDWPALP QLPGLHYSMN EQCRFDFGLG
510 520 530 540 550
YMMCTAFRTF DPCKQLWCSH PDNPYFCKTK KGPPLDGTMC APGKHCFKGH
560 570 580 590 600
CIWLTPDILK RDGNWGAWTP FGSCSRTCGT GVKFRTRQCD NPHPANGGRT
610 620 630 640 650
CSGLAYDFQL CNPQDCPNSL ADFREEQCQQ WDLYFEHGDV QHHWLPHEHR
660 670 680 690 700
DAKERCHLYC ESKETGEVVS MKRMVHDGTR CSYKDAFSLC VRGDCRKVGC
710 720 730 740 750
DGVIGSRKQE DKCGVCGGDN THCKVVKGTF TRSPRKQDYI KMFEIPAGAR
760 770 780 790 800
HLLIQEADTT SHHLSVKNLE TGKFILNEEN HLDPNSRSFI AMGVEWEYRN
810 820 830 840 850
EDERETLQTI GPLHGTITVL VIPEGDTRIS LTYKYMIHED SLNVDDNNVL
860 870 880 890 900
EDDAVRHEWA LKKWSPCSKP CGGGSQFTKY GCRRRLDSKM VHRAFCSALA
910 920 930 940 950
KPKAIRRACN PQECSQPVWV TGEWEPCTQS CGRTGMQVRS VRCIQPLHNN
960 970 980 990 1000
TTRSVHTKHC NDHRPESRRA CNRELCPGRW RAGSWSQCSV TCGNGTQERP
1010 1020 1030 1040 1050
VLCRTADDNF GVCREERPET ARICRLAPCP RNGSDPSKKS YVVQWLSRPD
1060 1070 1080 1090 1100
PDSPIQKISS KDQCQGDKSM FCRMEVLSRY CSIPSYNKLC CKSCNPPRNL
1110 1120 1130 1140 1150
SNTEDGGVEP PPGKHNDIDV FMPTLPGPTV ATQVQPSPGP PLEAPLNVSS
1160 1170 1180 1190 1200
TNATEDHPET NAVDVPYKIH GVDEEVPSPN LIPRRPSLYV KTRNQRIQEL
1210 1213
INAVQRKEKP GKF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3691 Collapse assay details

Data source: Panorama

Official Gene Symbol
Adamts2
Peptide Sequence
HLLIQEADTTSHHLSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
751
Peptide End
767
CPTAC ID
non-CPTAC-3691
Peptide Molecular Mass
1,928.0116
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 10.3 4.3 5.2 14.4 7 5.4 17.7 8.2 7.5 15 15 15
b4 (1+) 12 7.5 3.6 13.3 11.3 8.5 17.9 13.6 9.2 15 15 15
b3 (1+) 11.3 7.3 7.4 17.4 8.2 8 20.7 11 10.9 15 15 15
b2 (1+) 10.6 6.8 5.2 11.2 9.3 5.9 15.4 11.5 7.9 15 15 15
y10 (2+) 10.3 5.8 4.4 13.9 6.6 5.5 17.3 8.8 7 15 15 15
sum 5.1 2.7 1.8 9.5 5.7 3.2 10.8 6.3 3.7 15 15 15


Additional Resources and Comments