Dld, CPTAC-3704 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Dld	N/A

Sequence Length (AA)	Molecular Weight (Da)
509	54272

Protein Name
Dihydrolipoyl dehydrogenase, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQSWSRVYRS	LAKKGHFNRI	SHGLQGVSSV	PLRTYADQPI	EADVTVIGSG
60	70	80	90	100
PGGYVAAIKS	AQLGFKTVCI	EKNETLGGTC	LNVGCIPSKA	LLNNSHYYHM
110	120	130	140	150
AHGKDFASRG	IEIPEVRLNL	EKMMEQKHSA	VKALTGGIAH	LFKQNKVVHV
160	170	180	190	200
NGFGKITGKN	QVTATKADGS	TQVIDTKNIL	VATGSEVTPF	PGITIDEDTI
210	220	230	240	250
VSSTGALSLK	KVPEKLVVIG	AGVIGVELGS	VWQRLGADVT	AVEFLGHVGG
260	270	280	290	300
IGIDMEISKN	FQRILQRQGF	KFKLNTKVTG	ATKKSDGKID	VSVEAASGGK
310	320	330	340	350
AEVITCDVLL	VCIGRRPFTQ	NLGLEELGIE	LDPKGRIPVN	NRFQTKIPNI
360	370	380	390	400
YAIGDVVAGP	MLAHKAEDEG	IICVEGMAGG	AVHIDYNCVP	SVIYTHPEVA
410	420	430	440	450
WVGKSEEQLK	EEGIEFKIGK	FPFAANSRAK	TNADTDGMVK	ILGHKSTDRV
460	470	480	490	500
LGAHILGPGA	GEMVNEAALA	LEYGASCEDI	ARVCHAHPTL	SEAFREANLA
509
AAFGKPINF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3704 Collapse assay details

Data source: Panorama

Official Gene Symbol: Dld
Peptide Sequence: IDVSVEAASGGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 289
Peptide End: 300

CPTAC ID: non-CPTAC-3704
Peptide Molecular Mass: 1,131.5772
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	11.2	6.1	5	12.3	7.8	5.7	16.6	9.9	7.6	15	15	15
y4 (1+)	10.1	8.2	9.7	12.9	9	8	16.4	12.2	12.6	15	15	15
y7 (1+)	10	6.2	4.2	9.9	6.3	7.8	14.1	8.8	8.9	15	15	15
y9 (1+)	13.3	4.9	6.3	14	7.7	8.6	19.3	9.1	10.7	15	15	15
y10 (1+)	7.3	5.7	4.9	9	6.4	9.1	11.6	8.6	10.3	15	15	15
sum	4.2	2.2	3	6.1	4.2	6.2	7.4	4.7	6.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2727 Collapse assay details

Data source: Panorama

Official Gene Symbol: DLD
Peptide Sequence: ADGGTQVIDTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 167
Peptide End: 177

CPTAC ID: CPTAC-2727
Peptide Molecular Mass: 1,103.5459
Species: Homo Sapiens
Assay Type: Direct MRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend