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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Dsp

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dsp N/A
Sequence Length (AA) Molecular Weight (Da)
2883 332912
Protein Name
Desmoplakin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSCNGGSHPR INTLGRMTRA ESGPDLRYEM TYSGGGGGGG GGGGGGTSRT
60 70 80 90 100
FYSHSRRCTV NDQNSDGYCQ TGTMSRHQNQ NTIQEMLQNC SDCLMRAELI
110 120 130 140 150
AQPELKFGEG MQLAWNRELD EYFTQANDQM EIIDGLIREM RQMGQPCDAY
160 170 180 190 200
QKRLLQLQEQ MRALYKAISV PRVRRASSKG AGGYTCQSGS GWDEFTKRLT
210 220 230 240 250
GECLGWMRQQ REEMDLMAWG VDAGSVEQHI NSHRSIHNTI GDYRWQLDKI
260 270 280 290 300
KADLREKSAI YQLEEEYENL LKASFERMDH LRQLQNIIQA TSREIMWIND
310 320 330 340 350
CEEEELLYDW SDKNTNIAQK QEAFSIRMSQ LEVKEKELNK LKQESDQLVL
360 370 380 390 400
NQHPASDKIE AYMDTLQTQW SWILQITKCI DVHLKENAAY FQFFEEAQST
410 420 430 440 450
EAYLKGLQDS IRKKYPCDKN MPLQHLLEQI KELEKEREKI IEYKRQVQNL
460 470 480 490 500
VNKSKKIVQL KPRNPDYRSN KPIILRALCD YKQDQKIVHK GDECILKDNN
510 520 530 540 550
ERSKWYVTGP GGVDMLVPSV GLIIPPPNPL AVDLSCKIEQ YYEAILALWN
560 570 580 590 600
QLYINMKSLV SWHYCMIDIE KIRAMTIAKL KTMRQEDYMK TIEDLELHYQ
610 620 630 640 650
DFIKNSQGSE MFGDDDKRRM QSQFTDAQKH YQTLVIQLPG HPQHQTVTKT
660 670 680 690 700
EITHLGTCQD VNHNKVIETN RENDKQETWL LMELQKIRRQ MEHCEARMTL
710 720 730 740 750
KNLLLAEQGS THHITVKINE LKSVQNDSQA LAEVLNQLKD MLANFRGSEK
760 770 780 790 800
YCYLQNEIFG LFQKLENING VSDGYLNSLC SVRALLQAIL QTEDMLKVYE
810 820 830 840 850
ARLTEEETVC LDLDKVEAYR CGLKKIKNDL NLKKSLLATM KTELQKAQQI
860 870 880 890 900
HSQSSQQYPL YDLDLGKFTE KVTQLTDRWQ KIDKQIDFRL WDLEKQIKQL
910 920 930 940 950
RNYRDNYQSF CKWLYDAKRR QDSLESMKFG DSNTVMRFLN EQKNLHSEIS
960 970 980 990 1000
GKRDKSEEVH KIAELCANSI KDYELQLASY TSGLETLLNI PIKRTMVQSP
1010 1020 1030 1040 1050
SGVILQEAAD IHARYIELLT RSGDYYRFLS EMLKSLEDLK LKNTKIEVLE
1060 1070 1080 1090 1100
EELRLARDAN SENCNKNKFL DQNLQKYQAE CSQFKAKLVS LEELKRQAEL
1110 1120 1130 1140 1150
DGKSAKQNLD KCYGQIKELN EKITRLTYEI EDEKRRRKTV EDRFDQQKND
1160 1170 1180 1190 1200
YDQLQKARQC EKENLSWQKL ESEKAIKEKE YEIERLRVLL QEEGARKREY
1210 1220 1230 1240 1250
ENELAKVRNH YNEEMSNLRN KYETEINITK TTIKEISMQK EDDSKNLRNQ
1260 1270 1280 1290 1300
MDRLSRENRD LKDEIVRLND SILQATEQRR RAEENALQQK ACGSETMQKK
1310 1320 1330 1340 1350
QRLEIELKQV IQQRSEDNAR HKQSLEEAAK TIQDKNKEIE RLKAEYQEEA
1360 1370 1380 1390 1400
KRRWEYENEL SKVRNSYDEE IISLKNQFET EINITKTTIH QLTMQKEEDT
1410 1420 1430 1440 1450
SGYRAQIDNL TRENRSLCEE VKRLKNTLAQ TTENLRRVEE NAQQQKATGS
1460 1470 1480 1490 1500
EMSQRKQQLE IELRQVTQMR TEESMRYKQS LDDAAKTIQD KNKEIERLKQ
1510 1520 1530 1540 1550
LVDKETNERK CLEDENSKLQ RVQYDLQKAN NSATEAMSKL KVQEQELTRL
1560 1570 1580 1590 1600
RIDYERVSQE RTVKDQDITR IQSSLKDLQL QKQKAEEELS RLKRTASDES
1610 1620 1630 1640 1650
SKRKMLEEEL EAMRRSLKEQ AVKITNLTQQ LEQASIVKKR SEDDLRQQRD
1660 1670 1680 1690 1700
VLDGHVREKQ RTQEELRRLS LDVEALRRQL VQEQENVKQA HLRNEHFQKA
1710 1720 1730 1740 1750
IEDKSRSLNE SKIEIERLQS LTENLTKEHL MLEEELRNLR LEYDDLRRGR
1760 1770 1780 1790 1800
SEADSDKNST ISELRSQLQI SNNRTLELQG LINDLQRERE NLRQEIEKFQ
1810 1820 1830 1840 1850
KQALEASNRI QESKSQCTQV VQERESLLVK IKVLEQDKAR LQRLEDELNR
1860 1870 1880 1890 1900
AKATLEAESR VKQRLECEKQ QIQNDLNQWK TQYSRKEETI RKIESEREKS
1910 1920 1930 1940 1950
EREKNSLRSE IERLQAEIKR IEERCRRKLE DSSRETQSQL ESERCRLQKE
1960 1970 1980 1990 2000
IEKLRQRPYG SHRETQTEYE WTVDSSKLVF DGLRKKVTAM QLYECQLIDK
2010 2020 2030 2040 2050
TTLDKLLKGK KSVEEVASEI QPFLRGAGAI AGASASPKEK YSLVEAKRKK
2060 2070 2080 2090 2100
FITPESTVML LEAQAATGGI IDPHRNEKLT VDNAVARDLI DFDDRQQIYT
2110 2120 2130 2140 2150
AEKAITGFDD PFSGKTVSVS EAIKKNLIDR ETGMRLLEAQ LASGGVVDPV
2160 2170 2180 2190 2200
NSVFLPKDVA LARGLIDRDL YRSLNDPRDS QKNFVDPITK KKVSYMQLRE
2210 2220 2230 2240 2250
RCRIEPHTGL LLLSVQKRSM SFQGIRQPVT VTELVDSGIL RPSTVNELES
2260 2270 2280 2290 2300
GQISYDEVGE RIKDFLQGSS CIAGIYNETT KQKLGIYEAM KIGLVRPGTA
2310 2320 2330 2340 2350
LELLEAQAAT GFIVDPVSNL RLPVEEAYKR GLVGIEFKEK LLSAERAVTG
2360 2370 2380 2390 2400
YNDPETGNII SLFQAMNKEL IEKGHGIRLL EAQIATGGII DPKESHRLPV
2410 2420 2430 2440 2450
DMAYKRGYFN EELSEILSDP SDDTKGFFDP NTEENLTYLQ LKERCIKDEE
2460 2470 2480 2490 2500
TGLCLLPLKE KKKQVQTSQK NTLRKRRVVI VDPETNKEMS VQEAYKKGLI
2510 2520 2530 2540 2550
DYDTFKELCE QECEWEEITI TGSDGSTRVV LVDRKTGSQY DIQDAIDKGL
2560 2570 2580 2590 2600
VDRKFFDQYR SGSLSLTQFA DMISLKNGVG NSSGLGGSVN DDVFSSSRHD
2610 2620 2630 2640 2650
SVSKISTISS VRNLTIRSSS LSDPLEESSP IAAIFDTENL EKISIAEGIE
2660 2670 2680 2690 2700
RGIVDSITGQ RLLEAQACTG GIIHPTTGQK LSLQDAVNQG LIDQDMATRL
2710 2720 2730 2740 2750
KPAQKAFIGF EGVKGKKKMS AAEAVKEKWL PYEAGQRFLE FQFLTGGLVD
2760 2770 2780 2790 2800
PEVHGRISTE EAIRKGFIDG RAAQRLQDIS SYAKILTCPK TKLKISYKDA
2810 2820 2830 2840 2850
MNRSMVEDIT GLRLLEAASV SSKGLPSPYN MSAPGSRSGS RSGSRSGSRS
2860 2870 2880 2883
GSRSGSRRGS FDATGNSSYS YSYSFSSSSI GGY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-499 Collapse assay details

Data source: Panorama

Official Gene Symbol
DSP
Peptide Sequence
GFFDPNTEENLTYLQLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
2414
Peptide End
2430
CPTAC ID
CPTAC-499
Peptide Molecular Mass
2,027.9840
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 20.9 14.7 8.9 24.9 28.3 21.6 32.5 31.9 23.4 15 25 15
y6 (1+) 12.4 11.8 7.3 20.2 24.8 17.5 23.7 27.5 19 15 25 15
y13 (2+) 23.3 8.2 5.4 27 19.2 14.6 35.7 20.9 15.6 15 25 15
sum 8.6 8.6 5.2 12.9 21.7 15.7 15.5 23.3 16.5 15 25 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Assay Details for CPTAC-498 Collapse assay details

Data source: Panorama

Official Gene Symbol
DSP
Peptide Sequence
YGDGIQLTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
95
Peptide End
103
CPTAC ID
CPTAC-498
Peptide Molecular Mass
1,021.5193
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3703 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dsp
Peptide Sequence
AITGFDDPFSGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
2104
Peptide End
2115
CPTAC ID
non-CPTAC-3703
Peptide Molecular Mass
1,253.5928
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 10.9 3.6 2.2 12.2 6.2 8 16.4 7.2 8.3 15 15 15
y3 (1+) 14.8 9.6 8.5 14 11.8 8.5 20.4 15.2 12 15 15 15
y7 (1+) 16 6.5 4.4 23.3 6.6 7.7 28.3 9.3 8.9 15 15 15
y9 (1+) 11.4 3.7 6.6 14.6 2.7 7.9 18.5 4.6 10.3 15 15 15
y10 (1+) 7.7 5.2 4.3 10.2 6.1 7.2 12.8 8 8.4 15 15 15
sum 6.5 2.4 1.7 8.2 4.9 6.5 10.5 5.5 6.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments