Cst3, CPTAC-3702 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Cst3	N/A

Sequence Length (AA)	Molecular Weight (Da)
140	15531

Protein Name
Cystatin-C

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASPLRSLLF	LLAVLAVAWA	ATPKQGPRML	GAPEEADANE	EGVRRALDFA
60	70	80	90	100
VSEYNKGSND	AYHSRAIQVV	RARKQLVAGV	NYFLDVEMGR	TTCTKSQTNL
110	120	130	140
TDCPFHDQPH	LMRKALCSFQ	IYSVPWKGTH	SLTKFSCKNA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1114 Collapse assay details

Data source: Panorama

Official Gene Symbol: CST3
Peptide Sequence: ALDFAVGEYNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 52
Peptide End: 62

CPTAC ID: non-CPTAC-1114
Peptide Molecular Mass: 1,225.5979
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b5 (1+)	11.5	7.5	6.1	12.4	10.8	7.9	16.9	13.1	10	15	15	15
b3 (1+)	10.4	6	5.7	11.4	13.8	6.5	15.4	15	8.6	15	15	15
y6 (1+)	10.7	7.1	5.5	13.1	11.2	8.4	16.9	13.3	10	15	15	15
y7 (1+)	14.5	6.5	5.5	13.9	9.9	5.6	20.1	11.8	7.8	15	15	15
y9 (1+)	4.7	5.3	4	7.2	11.5	8.1	8.6	12.7	9	15	15	15
sum	4.5	4.8	3.5	5.3	10.3	5	7	11.4	6.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3702 Collapse assay details

Data source: Panorama

Official Gene Symbol: Cst3
Peptide Sequence: ALDFAVSEYNK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 46
Peptide End: 56

CPTAC ID: non-CPTAC-3702
Peptide Molecular Mass: 1,255.6085
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.