Csf1, CPTAC-3701 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Csf1	Csfm

Sequence Length (AA)	Molecular Weight (Da)
552	60649

Protein Name
Macrophage colony-stimulating factor 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTARGAAGRC	PSSTWLGSRL	LLVCLLMSRS	IAKEVSEHCS	HMIGNGHLKV
60	70	80	90	100
LQQLIDSQME	TSCQIAFEFV	DQEQLDDPVC	YLKKAFFLVQ	DIIDETMRFK
110	120	130	140	150
DNTPNANATE	RLQELSNNLN	SCFTKDYEEQ	NKACVRTFHE	TPLQLLEKIK
160	170	180	190	200
NFFNETKNLL	EKDWNIFTKN	CNNSFAKCSS	RDVVTKPDCN	CLYPKATPSS
210	220	230	240	250
DPASASPHQP	PAPSMAPLAG	LAWDDSQRTE	GSSLLPSELP	LRIEDPGSAK
260	270	280	290	300
QRPPRSTCQT	LESTEQPNHG	DRLTEDSQPH	PSAGGPVPGV	EDILESSLGT
310	320	330	340	350
NWVLEEASGE	ASEGFLTQEA	KFSPSTPVGG	SIQAETDRPR	ALSASPFPKS
360	370	380	390	400
TEDQKPVDIT	DRPLTEVNPM	RPIGQTQNNT	PEKTDGTSTL	REDHQEPGSP
410	420	430	440	450
HIATPNPQRV	SNSATPVAQL	LLPKSHSWGI	VLPLGELEGK	RSTRDRRSPA
460	470	480	490	500
ELEGGSASEG	AARPVARFNS	IPLTDTGHVE	QHEGSSDPQI	PESVFHLLVP
510	520	530	540	550
GIILVLLTVG	GLLFYKWKWR	SHRDPQTLDS	SVGRPEDSSL	TQDEDRQVEL
552
PV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-6245 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSF1
Peptide Sequence: SHSSGSVLPLGELEGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 428
Peptide End: 443

CPTAC ID: CPTAC-6245
Peptide Molecular Mass: 1,623.8216
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (1+)	12.7	13.6	7.5	10.6	9.8	10.2	16.5	16.8	12.7	15	15	15
y6 (1+)	11.6	7.4	7.5	10.5	10.3	10	15.6	12.7	12.5	15	15	15
y8 (1+)	9.2	8	4.3	8.9	10.3	3.9	12.8	13	5.8	15	15	15
b8 (1+)	6.8	7.9	5.6	6.9	8	5.2	9.7	11.2	7.6	15	15	15
y8 (2+)	9.2	6.1	3.3	7.7	7.3	7	12	9.5	7.7	15	15	15
sum	7.3	3.3	2	6.5	5.4	4.1	9.8	6.3	4.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for non-CPTAC-3701 Collapse assay details

Data source: Panorama

Official Gene Symbol: Csf1
Peptide Sequence: TFHETPLQLLEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 137
Peptide End: 148

CPTAC ID: non-CPTAC-3701
Peptide Molecular Mass: 1,454.7769
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.