CSF1, CPTAC-6245 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CSF1	N/A

Sequence Length (AA)	Molecular Weight (Da)
554	60179

Protein Name
Macrophage colony-stimulating factor 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTAPGAAGRC	PPTTWLGSLL	LLVCLLASRS	ITEEVSEYCS	HMIGSGHLQS
60	70	80	90	100
LQRLIDSQME	TSCQITFEFV	DQEQLKDPVC	YLKKAFLLVQ	DIMEDTMRFR
110	120	130	140	150
DNTPNAIAIV	QLQELSLRLK	SCFTKDYEEH	DKACVRTFYE	TPLQLLEKVK
160	170	180	190	200
NVFNETKNLL	DKDWNIFSKN	CNNSFAECSS	QDVVTKPDCN	CLYPKAIPSS
210	220	230	240	250
DPASVSPHQP	LAPSMAPVAG	LTWEDSEGTE	GSSLLPGEQP	LHTVDPGSAK
260	270	280	290	300
QRPPRSTCQS	FEPPETPVVK	DSTIGGSPQP	RPSVGAFNPG	MEDILDSAMG
310	320	330	340	350
TNWVPEEASG	EASEIPVPQG	TELSPSRPGG	GSMQTEPARP	SNFLSASSPL
360	370	380	390	400
PASAKGQQPA	DVTGTALPRV	GPVRPTGQDW	NHTPQKTDHP	SALLRDPPEP
410	420	430	440	450
GSPRISSLRP	QGLSNPSTLS	AQPQLSRSHS	SGSVLPLGEL	EGRRSTRDRR
460	470	480	490	500
SPAEPEGGPA	SEGAARPLPR	FNSVPLTDTG	HERQSEGSFS	PQLQESVFHL
510	520	530	540	550
LVPSVILVLL	AVGGLLFYRW	RRRSHQEPQR	ADSPLEQPEG	SPLTQDDRQV
554
ELPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6245 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSF1
Peptide Sequence: SHSSGSVLPLGELEGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 428
Peptide End: 443

CPTAC ID: CPTAC-6245
Peptide Molecular Mass: 1,623.8216
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (1+)	12.7	13.6	7.5	10.6	9.8	10.2	16.5	16.8	12.7	15	15	15
y6 (1+)	11.6	7.4	7.5	10.5	10.3	10	15.6	12.7	12.5	15	15	15
y8 (1+)	9.2	8	4.3	8.9	10.3	3.9	12.8	13	5.8	15	15	15
b8 (1+)	6.8	7.9	5.6	6.9	8	5.2	9.7	11.2	7.6	15	15	15
y8 (2+)	9.2	6.1	3.3	7.7	7.3	7	12	9.5	7.7	15	15	15
sum	7.3	3.3	2	6.5	5.4	4.1	9.8	6.3	4.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

View in CPTC Antibody Portal

Comments

Assay Details for non-CPTAC-3701 Collapse assay details

Data source: Panorama

Official Gene Symbol: Csf1
Peptide Sequence: TFHETPLQLLEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 137
Peptide End: 148

CPTAC ID: non-CPTAC-3701
Peptide Molecular Mass: 1,454.7769
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.