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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fst N/A
Sequence Length (AA) Molecular Weight (Da)
344 37866
Protein Name
Follistatin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVCARHQPGG LCLLLLLLCQ FMEDRSAQAG NCWLRQAKNG RCQVLYKTEL
60 70 80 90 100
SKEECCSTGR LSTSWTEEDV NDNTLFKWMI FNGGAPNCIP CKETCENVDC
110 120 130 140 150
GPGKKCRMNK KNKPRCVCAP DCSNITWKGP VCGLDGKTYR NECALLKARC
160 170 180 190 200
KEQPELEVQY QGKCKKTCRD VFCPGSSTCV VDQTNNAYCV TCNRICPEPS
210 220 230 240 250
SSEQYLCGND GVTYSSACHL RKATCLLGRS IGLAYEGKCI KAKSCEDIQC
260 270 280 290 300
GGGKKCLWDS KVGRGRCSLC DELCPDSKSD EPVCASDNAT YASECAMKEA
310 320 330 340 344
ACSSGVLLEV KHSGSCNSIS EETEEEEEEE DQDYSFPISS ILEW

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3710 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fst
Peptide Sequence
EQPELEVQYQGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
152
Peptide End
163
CPTAC ID
non-CPTAC-3710
Peptide Molecular Mass
1,446.6991
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 26.4 6.7 8 19 12.3 8.9 32.5 14 12 15 15 15
y10 (2+) 11.9 6.3 5.5 12 9 7.7 16.9 11 9.5 15 15 15
y2 (1+) 34.7 9 13.2 38.1 11.2 14.8 51.5 14.4 19.8 15 15 15
y3 (1+) 19.7 10.6 10.4 27.8 13.5 14.4 34.1 17.2 17.8 15 15 15
y10 (1+) 17.2 7.4 6.2 17.5 7.6 6.8 24.5 10.6 9.2 15 15 15
sum 8.6 5.1 4.3 9.9 6.9 6.5 13.1 8.6 7.8 15 15 15

Additional Resources and Comments