Fst, CPTAC-3710 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Fst	N/A

Sequence Length (AA)	Molecular Weight (Da)
344	37866

Protein Name
Follistatin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVCARHQPGG	LCLLLLLLCQ	FMEDRSAQAG	NCWLRQAKNG	RCQVLYKTEL
60	70	80	90	100
SKEECCSTGR	LSTSWTEEDV	NDNTLFKWMI	FNGGAPNCIP	CKETCENVDC
110	120	130	140	150
GPGKKCRMNK	KNKPRCVCAP	DCSNITWKGP	VCGLDGKTYR	NECALLKARC
160	170	180	190	200
KEQPELEVQY	QGKCKKTCRD	VFCPGSSTCV	VDQTNNAYCV	TCNRICPEPS
210	220	230	240	250
SSEQYLCGND	GVTYSSACHL	RKATCLLGRS	IGLAYEGKCI	KAKSCEDIQC
260	270	280	290	300
GGGKKCLWDS	KVGRGRCSLC	DELCPDSKSD	EPVCASDNAT	YASECAMKEA
310	320	330	340	344
ACSSGVLLEV	KHSGSCNSIS	EETEEEEEEE	DQDYSFPISS	ILEW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3710 Collapse assay details

Data source: Panorama

Official Gene Symbol: Fst
Peptide Sequence: EQPELEVQYQGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 152
Peptide End: 163

CPTAC ID: non-CPTAC-3710
Peptide Molecular Mass: 1,446.6991
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	26.4	6.7	8	19	12.3	8.9	32.5	14	12	15	15	15
y10 (2+)	11.9	6.3	5.5	12	9	7.7	16.9	11	9.5	15	15	15
y2 (1+)	34.7	9	13.2	38.1	11.2	14.8	51.5	14.4	19.8	15	15	15
y3 (1+)	19.7	10.6	10.4	27.8	13.5	14.4	34.1	17.2	17.8	15	15	15
y10 (1+)	17.2	7.4	6.2	17.5	7.6	6.8	24.5	10.6	9.2	15	15	15
sum	8.6	5.1	4.3	9.9	6.9	6.5	13.1	8.6	7.8	15	15	15

Fst

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3710 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Fst

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3710 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments