Ldhb, CPTAC-3720 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Ldhb	Ldh-2, Ldh2

Sequence Length (AA)	Molecular Weight (Da)
334	36572

Protein Name
L-lactate dehydrogenase B chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATLKEKLIA	SVADDEAAVP	NNKITVVGVG	QVGMACAISI	LGKSLADELA
60	70	80	90	100
LVDVLEDKLK	GEMMDLQHGS	LFLQTPKIVA	DKDYSVTANS	KIVVVTAGVR
110	120	130	140	150
QQEGESRLNL	VQRNVNVFKF	IIPQIVKYSP	DCTIIVVSNP	VDILTYVTWK
160	170	180	190	200
LSGLPKHRVI	GSGCNLDSAR	FRYLMAEKLG	IHPSSCHGWI	LGEHGDSSVA
210	220	230	240	250
VWSGVNVAGV	SLQELNPEMG	TDNDSENWKE	VHKMVVDSAY	EVIKLKGYTN
260	270	280	290	300
WAIGLSVADL	IESMLKNLSR	IHPVSTMVKG	MYGIENEVFL	SLPCILNARG
310	320	330	334
LTSVINQKLK	DDEVAQLRKS	ADTLWDIQKD	LKDL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3720 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ldhb
Peptide Sequence: IVVVTAGVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 92
Peptide End: 100

CPTAC ID: non-CPTAC-3720
Peptide Molecular Mass: 912.5757
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	7.4	4.3	4.4	13.2	5.2	6.5	15.1	6.7	7.8	15	15	15
y7 (1+)	9.6	6.4	5.7	12.3	6.5	7.1	15.6	9.1	9.1	15	15	15
b3 (1+)	33.8	10.2	4.7	48.4	15.6	6.4	59	18.6	7.9	15	15	15
b2 (1+)	29.4	6.5	4.6	41.1	8.2	5.1	50.5	10.5	6.9	15	15	15
y7 (2+)	30	11.9	6.1	37.5	11.5	8.6	48	16.5	10.5	15	15	15
sum	23.6	4.9	3.8	32.6	5.5	4.2	40.2	7.4	5.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2751 Collapse assay details

Data source: Panorama

Official Gene Symbol: LDHB
Peptide Sequence: FIIPQIVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 120
Peptide End: 127

CPTAC ID: CPTAC-2751
Peptide Molecular Mass: 956.6059
Species: Homo Sapiens
Assay Type: Direct MRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend