Pnliprp1, CPTAC-3721 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Pnliprp1	N/A

Sequence Length (AA)	Molecular Weight (Da)
473	52696

Protein Name
Inactive pancreatic lipase-related protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLILWTIPLF	LLGAAQGKEV	CYDNLGCFSD	AEPWAGTAIR	PLKLLPWSPE
60	70	80	90	100
KINTRFLLYT	NENPTAFQTL	QLSDPSTIEA	SNFQVARKTR	FIIHGFIDKG
110	120	130	140	150
EENWVVDMCK	NMFQVEEVNC	ICVDWKRGSQ	TTYTQAANNV	RVVGAQVAQM
160	170	180	190	200
IDILVRNFNY	SASKVHLIGH	SLGAHVAGEA	GSRTPGLGRI	TGLDPVEANF
210	220	230	240	250
EGTPEEVRLD	PSDADFVDVI	HTDAAPLIPF	LGFGTNQMVG	HFDFFPNGGQ
260	270	280	290	300
YMPGCKKNAL	SQIVDIDGIW	SGTRDFVACN	HLRSYKYYLE	SILNPDGFAA
310	320	330	340	350
YPCASYRDFE	SNKCFPCPDQ	GCPQMGHYAD	KFANNTSVEP	QKFFLNTGEA
360	370	380	390	400
KNFARWRYRV	SLTFSGRTVT	GQVKVSLFGS	NGNTRQCDIF	RGIIKPGATH
410	420	430	440	450
SNEFDAKLDV	GTIEKVKFLW	NNHVVNPSFP	KVGAAKITVQ	KGEERTEHNF
460	470	473
CSEETVREDI	LLTLLPCKTS	DTM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3721 Collapse assay details

Data source: Panorama

Official Gene Symbol: Pnliprp1
Peptide Sequence: VHLIGHSLGAHVAGEAGSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 165
Peptide End: 183

CPTAC ID: non-CPTAC-3721
Peptide Molecular Mass: 1,866.9813
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	14.3	5.8	7	11.3	7.7	8.8	18.2	9.6	11.2	15	15	15
y18 (4+)	35.2	8.3	10.4	39.5	10.6	11.8	52.9	13.5	15.7	15	15	15
y17 (3+)	19.4	8.5	7.1	20.9	11.6	6.1	28.5	14.4	9.4	15	15	15
y6 (1+)	23.3	6.6	7.3	25.9	10.9	9.3	34.8	12.7	11.8	15	15	15
y7 (1+)	15.5	6.6	2.6	13.1	6.1	6.6	20.3	9	7.1	15	15	15
sum	10.4	2.5	3.1	10.3	5.6	4.3	14.6	6.1	5.3	15	15	15

Pnliprp1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3721 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Pnliprp1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3721 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments