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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Rpl10a Nedd-6, Nedd6
Sequence Length (AA) Molecular Weight (Da)
217 24916
Protein Name
60S ribosomal protein L10a
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSSKVSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS
60 70 80 90 100
GTVRLKSTPR PKFSVCVLGD QQHCDERKAV DIPHMDIEAL KKLNKNKKLV
110 120 130 140 150
KKLAKKYDAF LASESLIKQI PRILGPGLNK AGKFPSLLTH NENMVAKVDE
160 170 180 190 200
VKSTIKFQMK KVLCLAVAVG HVKMTDDELV YNIHLAVNFL VSLLKKNWQN
210 217
VRALYIKSTM GKPQRLY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3742 Collapse assay details

Data source: Panorama

Official Gene Symbol
Rpl10a
Peptide Sequence
ILGPGLNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
123
Peptide End
130
CPTAC ID
non-CPTAC-3742
Peptide Molecular Mass
810.4963
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 24.5 9 6.4 28.8 11.9 9.8 37.8 14.9 11.7 15 15 15
y6 (2+) 28 9.5 14 21.3 10.1 15.7 35.2 13.9 21 15 15 15
y5 (1+) 20.7 8.5 3.6 18.5 8.6 7.4 27.8 12.1 8.2 15 15 15
y6 (1+) 4.9 3 3.4 7.7 5.1 8 9.1 5.9 8.7 15 15 15
y7 (1+) 9.6 8.9 4.9 22.7 7.9 8.4 24.6 11.9 9.7 15 15 15
sum 5 2.7 2.8 10.1 4.6 7.3 11.3 5.3 7.8 15 15 15

Additional Resources and Comments