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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Flt4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Flt4 Flt-4, Vegfr3
Sequence Length (AA) Molecular Weight (Da)
1363 153016
Protein Name
Vascular endothelial growth factor receptor 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPGAALNLR LWLCLGLLQG LANGYSMTPP TLNITEDSYV IDTGDSLSIS
60 70 80 90 100
CRGQHPLEWT WPGAQEVLTT GGKDSEDTRV VHDCEGTEAR PYCKVLLLAQ
110 120 130 140 150
THANNTGSYH CYYKYIKARI EGTTAASTYV FVRDFKHPFI NKPDTLLVNR
160 170 180 190 200
KDSMWVPCLV SIPGLNITLR SQSSALHPDG QEVLWDDRRG MRVPTQLLRD
210 220 230 240 250
ALYLQCETTW GDQNFLSNLF VVHITGNELY DIQLYPKKSM ELLVGEKLVL
260 270 280 290 300
NCTVWAEFDS GVTFDWDYPG KQAERAKWVP ERRSQQTHTE LSSILTIHNV
310 320 330 340 350
SQNDLGPYVC EANNGIQRFR ESTEVIVHEK PFISVEWLKG PVLEATAGDE
360 370 380 390 400
LVKLPVKLAA YPPPEFQWYK DRKAVTGRHN PHALVLKEVT EASAGVYTLA
410 420 430 440 450
LWNSAAGLRQ NISLELVVNV PPHIHEKEAS SPSIYSRHSR QTLTCTAYGV
460 470 480 490 500
PQPLSVQWHW RPWTPCKTFA QRSLRRRQQR DGMPQCRDWK EVTTQDAVNP
510 520 530 540 550
IESLDSWTEF VEGKNKTVSK LVIQDANVSA MYKCVVVNKV GQDERLIYFY
560 570 580 590 600
VTTIPDGFSI ESEPSEDPLE GQSVRLSCRA DNYTYEHLRW YRLNLSTLHD
610 620 630 640 650
AQGNPLLLDC KNVHLFATPL EANLEEAEPG ARHATLSLNI PRVAPEDEGD
660 670 680 690 700
YVCEVQDRRS QDKHCHKKYL SVQALEAPRL TQNLTDLLVN VSDSLEMRCP
710 720 730 740 750
VAGAHVPSIV WYKDERLLEK ESGIDLADSN QRLSIQRVRE EDAGRYLCSV
760 770 780 790 800
CNAKGCVNSS ASVAVEGSED KGSMEIVILI GTGVIAVFFW VLLLLIFCNM
810 820 830 840 850
KRPAHADIKT GYLSIIMDPG EVPLEEQCEY LSYDASQWEF PRERLHLGRV
860 870 880 890 900
LGHGAFGKVV EASAFGINKG SSCDTVAVKM LKEGATASEH RALMSELKIL
910 920 930 940 950
IHIGNHLNVV NLLGACTKPN GPLMVIVEFC KYGNLSNFLR VKRDTFNPYA
960 970 980 990 1000
EKSPEQRRRF RAMVEGAKAD RRRPGSSDRA LFTRFLMGKG SARRAPLVQE
1010 1020 1030 1040 1050
AEDLWLSPLT MEDLVCYSFQ VARGMEFLAS RKCIHRDLAA RNILLSESDI
1060 1070 1080 1090 1100
VKICDFGLAR DIYKDPDYVR KGSARLPLKW MAPESIFDKV YTTQSDVWSF
1110 1120 1130 1140 1150
GVLLWEIFSL GASPYPGVQI NEEFCQRLKD GTRMRAPELA TPAIRHIMQS
1160 1170 1180 1190 1200
CWSGDPKARP AFSDLVEILG DLLQGGGWQE EEEERMALHS SQSSEEDGFM
1210 1220 1230 1240 1250
QASTTALHIT EADADDSPPS MHCHSLAARY YNCVSFPGRL ARGTKTPGSS
1260 1270 1280 1290 1300
RMKTFEELPM TPTTYKASMD NQTDSGMVLA SEEFEELESR HRPEGSFSCK
1310 1320 1330 1340 1350
GPGQHMDIPR GHPDPQGRRR RPTQGAQGGK VFYNNEYGEV SQPCTEGDCC
1360 1363
PSAGSTFFAD SSY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3753 Collapse assay details

Data source: Panorama

Official Gene Symbol
Flt4
Peptide Sequence
ESGIDLADSNQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
721
Peptide End
732
CPTAC ID
non-CPTAC-3753
Peptide Molecular Mass
1,303.6004
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 17.2 2 2.3 19.8 5.5 3.1 26.2 5.9 3.9 15 15 15
y7 (1+) 20.1 3.6 4.4 14.2 6.7 7.6 24.6 7.6 8.8 15 15 15
y8 (1+) 13.2 6.1 2.8 16.8 7.2 6.3 21.4 9.4 6.9 15 15 15
y4 (1+) 15.6 5 2.9 16.1 5.8 4.4 22.4 7.7 5.3 15 15 15
y5 (1+) 8.8 3.4 3 10.2 4.6 5.9 13.5 5.7 6.6 15 15 15
sum 7 3.1 1.4 9.3 4.5 3.5 11.6 5.5 3.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments