Vta1, CPTAC-3754 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Vta1	N/A

Sequence Length (AA)	Molecular Weight (Da)
309	33913

Protein Name
Vacuolar protein sorting-associated protein VTA1 homolog

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAALAPLPPL	PAQFKSIQHH	LRTAQEHDKR	DPVVAYYCRL	YAMQTGMKID
60	70	80	90	100
SKTPECRKFL	SKLMDQLEAL	KKQLGDNEAV	TQEIVGCAHL	ENYALKMFLY
110	120	130	140	150
ADNEDRAGRF	HKNMIKSFYT	ASLLIDVITV	FGELTDENVK	HRKYARWKAT
160	170	180	190	200
YIHNCLKNGE	TPQAGPVGIE	EENDVEENED	VGATSLPTQP	PQPSSSSAYD
210	220	230	240	250
PSNLAPGSYS	GIQIPPGAHA	PANTPAEVPH	STGVTSNAVQ	PSPQTVPAAP
260	270	280	290	300
AVDPDLYTAS	QGDIRLTPED	FARAQKYCKY	AGSALQYEDV	GTAVQNLQKA
309
LRLLTTGRE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3754 Collapse assay details

Data source: Panorama

Official Gene Symbol: Vta1
Peptide Sequence: LTPEDFAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 266
Peptide End: 273

CPTAC ID: non-CPTAC-3754
Peptide Molecular Mass: 947.4712
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	20	18.2	7	25.5	22.2	11.2	32.4	28.7	13.2	15	15	15
y6 (2+)	20.6	12.4	9.8	27.3	12.3	11.2	34.2	17.5	14.9	15	15	15
y4 (1+)	25.5	15.6	8.8	28.3	15.8	8.4	38.1	22.2	12.2	15	15	15
y5 (1+)	24	14.5	14.5	30.4	17.3	14.7	38.7	22.6	20.6	15	15	15
y6 (1+)	10.4	7.2	6.2	15.8	13	12.7	18.9	14.9	14.1	15	15	15
sum	5.9	5.4	4.6	15.1	10.1	8.8	16.2	11.5	9.9	15	15	15

Vta1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3754 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

Vta1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3754 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments