C1qb, CPTAC-5579 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C1qb	N/A

Sequence Length (AA)	Molecular Weight (Da)
253	26717

Protein Name
Complement C1q subcomponent subunit B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKTQWGEVWT	HLLLLLLGFL	HVSWAQSSCT	GPPGIPGIPG	VPGVPGSDGQ
60	70	80	90	100
PGTPGIKGEK	GLPGLAGDLG	EFGEKGDPGI	PGTPGKVGPK	GPVGPKGTPG
110	120	130	140	150
PSGPRGPKGD	SGDYGATQKV	AFSALRTINS	PLRPNQVIRF	EKVITNANEN
160	170	180	190	200
YEPRNGKFTC	KVPGLYYFTY	HASSRGNLCV	NLVRGRDRDS	MQKVVTFCDY
210	220	230	240	250
AQNTFQVTTG	GVVLKLEQEE	VVHLQATDKN	SLLGIEGANS	IFTGFLLFPD
253
MDA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1101 Collapse assay details

Data source: Panorama

Official Gene Symbol: C1QB
Peptide Sequence: IAFSATR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 122
Peptide End: 128

CPTAC ID: non-CPTAC-1101
Peptide Molecular Mass: 764.4181
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	8.8	5.6	7	9	9	7.5	12.6	10.6	10.3	15	15	15
y5 (1+)	9.5	7.2	7.3	10.5	7.4	7	14.2	10.3	10.1	15	15	15
y6 (1+)	11.7	9	6.1	15.9	9.1	6.6	19.7	12.8	9	15	15	15
sum	6	5.3	5.4	7.3	5.4	5.5	9.4	7.6	7.7	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5579 Collapse assay details

Data source: Panorama

Official Gene Symbol: C1qb
Peptide Sequence: VITNANENYEPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 143
Peptide End: 154

CPTAC ID: non-CPTAC-5579
Peptide Molecular Mass: 1,418.6790
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.