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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C1QB N/A
Sequence Length (AA) Molecular Weight (Da)
253 26722
Protein Name
Complement C1q subcomponent subunit B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMMKIPWGSI PVLMLLLLLG LIDISQAQLS CTGPPAIPGI PGIPGTPGPD
60 70 80 90 100
GQPGTPGIKG EKGLPGLAGD HGEFGEKGDP GIPGNPGKVG PKGPMGPKGG
110 120 130 140 150
PGAPGAPGPK GESGDYKATQ KIAFSATRTI NVPLRRDQTI RFDHVITNMN
160 170 180 190 200
NNYEPRSGKF TCKVPGLYYF TYHASSRGNL CVNLMRGRER AQKVVTFCDY
210 220 230 240 250
AYNTFQVTTG GMVLKLEQGE NVFLQATDKN SLLGMEGANS IFSGFLLFPD
253
MEA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1101 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1QB
Peptide Sequence
IAFSATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
122
Peptide End
128
CPTAC ID
non-CPTAC-1101
Peptide Molecular Mass
764.4181
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 8.8 5.6 7 9 9 7.5 12.6 10.6 10.3 15 15 15
y5 (1+) 9.5 7.2 7.3 10.5 7.4 7 14.2 10.3 10.1 15 15 15
y6 (1+) 11.7 9 6.1 15.9 9.1 6.6 19.7 12.8 9 15 15 15
sum 6 5.3 5.4 7.3 5.4 5.5 9.4 7.6 7.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5579 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1qb
Peptide Sequence
VITNANENYEPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
143
Peptide End
154
CPTAC ID
non-CPTAC-5579
Peptide Molecular Mass
1,418.6790
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 16.5 7.3 5.2 22.8 8.6 6.5 28.1 11.3 8.3 15 15 15
y2 (1+) 12.1 6.1 3.2 15.8 8.7 6.4 19.9 10.6 7.2 15 15 15
y7 (1+) 13 3.9 3.1 13.1 7.8 4.6 18.5 8.7 5.5 15 15 15
y8 (1+) 18.9 6.8 3.7 20.4 11.4 5.2 27.8 13.3 6.4 15 15 15
y9 (1+) 17.5 6.3 3.3 17.4 8.2 3.7 24.7 10.3 5 15 15 15
sum 5.6 2.6 2.1 10.1 6.6 3.5 11.5 7.1 4.1 15 15 15

Additional Resources and Comments