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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C1RL

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C1RL C1RL1, C1RLP, CLSPA
Sequence Length (AA) Molecular Weight (Da)
487 53498
Protein Name
Complement C1r subcomponent-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPGPRVWGKY LWRSPHSKGC PGAMWWLLLW GVLQACPTRG SVLLAQELPQ
60 70 80 90 100
QLTSPGYPEP YGKGQESSTD IKAPEGFAVR LVFQDFDLEP SQDCAGDSVT
110 120 130 140 150
ISFVGSDPSQ FCGQQGSPLG RPPGQREFVS SGRSLRLTFR TQPSSENKTA
160 170 180 190 200
HLHKGFLALY QTVAVNYSQP ISEASRGSEA INAPGDNPAK VQNHCQEPYY
210 220 230 240 250
QAAAAGALTC ATPGTWKDRQ DGEEVLQCMP VCGRPVTPIA QNQTTLGSSR
260 270 280 290 300
AKLGNFPWQA FTSIHGRGGG ALLGDRWILT AAHTIYPKDS VSLRKNQSVN
310 320 330 340 350
VFLGHTAIDE MLKLGNHPVH RVVVHPDYRQ NESHNFSGDI ALLELQHSIP
360 370 380 390 400
LGPNVLPVCL PDNETLYRSG LLGYVSGFGM EMGWLTTELK YSRLPVAPRE
410 420 430 440 450
ACNAWLQKRQ RPEVFSDNMF CVGDETQRHS VCQGDSGSVY VVWDNHAHHW
460 470 480 487
VATGIVSWGI GCGEGYDFYT KVLSYVDWIK GVMNGKN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3895 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1rl
Peptide Sequence
GQESHTDIEAPEGFAVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
67
Peptide End
83
CPTAC ID
non-CPTAC-3895
Peptide Molecular Mass
1,841.8544
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b10 (2+) 9.3 5.8 5.8 8.8 6.1 9.8 12.8 8.4 11.4 15 15 15
y7 (2+) 11.6 6.2 5.2 13.4 8.7 7.1 17.7 10.7 8.8 15 15 15
y8 (2+) 16.8 10.7 8.6 18.6 9.5 8 25.1 14.3 11.7 15 15 15
y5 (1+) 12 9.3 12.4 11.3 14 14.3 16.5 16.8 18.9 15 15 15
y7 (1+) 28.5 17.8 10.2 32.9 16.8 10.3 43.5 24.5 14.5 15 15 15
sum 5.6 4.6 3.2 7.5 6.4 6.2 9.4 7.9 7 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-4018 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1rl
Peptide Sequence
GYGFYTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
460
Peptide End
466
CPTAC ID
non-CPTAC-4018
Peptide Molecular Mass
834.3912
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 7.7 2.3 5.1 7.7 4.5 7 10.9 5.1 8.7 15 15 15
y3 (2+) 134.4 89.1 100.8 151.1 104.4 118.1 202.2 137.3 155.3 15 15 15
y6 (2+) 23.2 10.5 8.2 22.3 15.2 10 32.2 18.5 12.9 15 15 15
y2 (1+) 9.6 5.6 4 19 6.8 6.9 21.3 8.8 8 15 15 15
y5 (1+) 3.4 4.3 4.7 7.9 5.7 4.7 8.6 7.1 6.6 15 15 15
sum 2.3 3.1 3.2 7.2 4.9 4.2 7.6 5.8 5.3 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-1102 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1RL
Peptide Sequence
VVVHPDYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
317
Peptide End
324
CPTAC ID
non-CPTAC-1102
Peptide Molecular Mass
983.5189
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (2+) 16.4 9.4 7.5 15.6 9.5 7.1 22.6 13.4 10.3 15 15 15
y6 (2+) 9 7.2 6.9 8.8 9.4 8.5 12.6 11.8 10.9 15 15 15
y7 (2+) 7.7 9.4 7.2 9 9 5.1 11.8 13 8.8 15 15 15
y4 (1+) 10 9.2 7.5 12.7 9.8 7 16.2 13.4 10.3 15 15 15
y5 (1+) 21.2 8.6 8.1 14.6 6 8.8 25.7 10.5 12 15 15 15
sum 4.9 5 3.4 5.1 5.7 3.6 7.1 7.6 5 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-3429 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1rl
Peptide Sequence
VVVHPDYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
317
Peptide End
324
CPTAC ID
non-CPTAC-3429
Peptide Molecular Mass
983.5189
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (2+) 8.3 8.4 7.6 17.7 13.9 8.5 19.5 16.2 11.4 15 15 15
y6 (2+) 7.7 5.3 6.3 15.7 8.1 8.2 17.5 9.7 10.3 15 15 15
y7 (2+) 8.7 7 4.1 15 9.7 7 17.3 12 8.1 15 15 15
y4 (1+) 6.3 5 7.4 15 10.7 9.5 16.3 11.8 12 15 15 15
y5 (1+) 8.6 9.2 5 19.8 10.1 8.3 21.6 13.7 9.7 15 15 15
sum 2.9 2.7 4.2 14 7.7 6.4 14.3 8.2 7.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments