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Overview Data source: UniProt

Official Gene Symbol Other Aliases
F12 N/A
Sequence Length (AA) Molecular Weight (Da)
615 67792
Protein Name
Coagulation factor XII
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRALLLLGFL LVSLESTLSI PPWEAPKEHK YKAEEHTVVL TVTGEPCHFP
60 70 80 90 100
FQYHRQLYHK CTHKGRPGPQ PWCATTPNFD QDQRWGYCLE PKKVKDHCSK
110 120 130 140 150
HSPCQKGGTC VNMPSGPHCL CPQHLTGNHC QKEKCFEPQL LRFFHKNEIW
160 170 180 190 200
YRTEQAAVAR CQCKGPDAHC QRLASQACRT NPCLHGGRCL EVEGHRLCHC
210 220 230 240 250
PVGYTGAFCD VDTKASCYDG RGLSYRGLAR TTLSGAPCQP WASEATYRNV
260 270 280 290 300
TAEQARNWGL GGHAFCRNPD NDIRPWCFVL NRDRLSWEYC DLAQCQTPTQ
310 320 330 340 350
AAPPTPVSPR LHVPLMPAQP APPKPQPTTR TPPQSQTPGA LPAKREQPPS
360 370 380 390 400
LTRNGPLSCG QRLRKSLSSM TRVVGGLVAL RGAHPYIAAL YWGHSFCAGS
410 420 430 440 450
LIAPCWVLTA AHCLQDRPAP EDLTVVLGQE RRNHSCEPCQ TLAVRSYRLH
460 470 480 490 500
EAFSPVSYQH DLALLRLQED ADGSCALLSP YVQPVCLPSG AARPSETTLC
510 520 530 540 550
QVAGWGHQFE GAEEYASFLQ EAQVPFLSLE RCSAPDVHGS SILPGMLCAG
560 570 580 590 600
FLEGGTDACQ GDSGGPLVCE DQAAERRLTL QGIISWGSGC GDRNKPGVYT
610 615
DVAYYLAWIR EHTVS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3541 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
DAPDGPTVVLTVDGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
45
CPTAC ID
non-CPTAC-3541
Peptide Molecular Mass
1,510.7627
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 3.6 3.2 2.5 11.4 3.2 6.7 12 4.5 7.2 15 15 15
y2 (1+) 4.9 2.9 2.8 19 7.9 11.5 19.6 8.4 11.8 15 15 15
y6 (1+) 6.6 5.3 4.2 10.9 4.9 9.7 12.7 7.2 10.6 15 15 15
y7 (1+) 4.4 3.2 2.2 15.7 4.4 4.7 16.3 5.4 5.2 15 15 15
y10 (1+) 6.2 5.4 4.6 12.2 8 12.1 13.7 9.7 12.9 15 15 15
sum 3.1 1.6 1.7 12 3 6 12.4 3.4 6.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2650 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
EQPPSLTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
346
Peptide End
353
CPTAC ID
non-CPTAC-2650
Peptide Molecular Mass
926.4821
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 10.1 7 4.7 15 9.7 6.2 18.1 12 7.8 15 15 15
b2 (1+) 19.6 5.1 6.8 27.5 10.1 8 33.8 11.3 10.5 15 15 15
y5 (2+) 25.2 9.7 8.2 41 11.6 10.4 48.1 15.1 13.2 15 15 15
y6 (1+) 12.5 5.8 6.9 14 10.1 8.1 18.8 11.6 10.6 15 15 15
y5 (1+) 13.8 5.7 6.8 16.5 7.3 8.3 21.5 9.3 10.7 15 15 15
sum 7.4 5.4 3.9 13.5 8.8 5.9 15.4 10.3 7.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3707 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
TGPGGVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
153
Peptide End
160
CPTAC ID
non-CPTAC-3707
Peptide Molecular Mass
713.3820
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 8.4 7.9 8.1 17 11.6 8.1 19 14 11.5 15 15 15
y4 (1+) 31.3 26.8 13.6 36.5 35.3 19.8 48.1 44.3 24 15 15 15
y5 (1+) 8.6 14.3 5.2 21.4 13.8 8.5 23.1 19.9 10 15 15 15
y6 (1+) 16.7 14 7.4 14.9 18 11.9 22.4 22.8 14 15 15 15
y7 (1+) 25.7 57.4 10.1 24.4 46.1 20.3 35.4 73.6 22.7 15 15 15
sum 5.5 5 5.4 16.5 10.5 5.1 17.4 11.6 7.4 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1100 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
VVGGLVALR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
373
Peptide End
381
CPTAC ID
non-CPTAC-1100
Peptide Molecular Mass
882.5651
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 10.9 6.2 4.9 18.5 6.6 5.9 21.5 9.1 7.7 15 15 15
y7 (1+) 5.8 3.6 5.1 10.8 7.6 6 12.3 8.4 7.9 15 15 15
y8 (1+) 17.4 4.5 4.3 15.3 4.4 5.7 23.2 6.3 7.1 15 15 15
sum 5 3.4 4.3 10.8 6.9 4.7 11.9 7.7 6.4 15 15 15

Additional Resources and Comments