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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
F12 N/A
Sequence Length (AA) Molecular Weight (Da)
597 65701
Protein Name
Coagulation factor XII
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTALLFLGSL LMSLDLTLSA PPWKDSKKFK DAPDGPTVVL TVDGRLCHFP
60 70 80 90 100
FQYHRQLHHK CIHKRRPGSR PWCATTPNFD EDQQWGYCLE PKKVKDHCSK
110 120 130 140 150
HNPCHKGGTC INTPNGPHCL CPEHLTGKHC QKEKCFEPQL LKFFHENELW
160 170 180 190 200
FRTGPGGVAR CECKGSEAHC KPVASQACSI NPCLNGGSCL LVEDHPLCRC
210 220 230 240 250
PTGYTGYFCD LDLWATCYEG RGLSYRGQAG TTQSGAPCQR WTVEATYRNM
260 270 280 290 300
TEKQALSWGL GHHAFCRNPD NDTRPWCFVW SGDRLSWDYC GLEQCQTPTF
310 320 330 340 350
APLVVPESQE ESPSQAPSLS HAPNDSTDHQ TSLSKTNTMG CGQRFRKGLS
360 370 380 390 400
SFMRVVGGLV ALPGSHPYIA ALYWGNNFCA GSLIAPCWVL TAAHCLQNRP
410 420 430 440 450
APEELTVVLG QDRHNQSCEW CQTLAVRSYR LHEGFSSITY QHDLALLRLQ
460 470 480 490 500
ESKTNSCAIL SPHVQPVCLP SGAAPPSETV LCEVAGWGHQ FEGAEEYSTF
510 520 530 540 550
LQEAQVPFIA LDRCSNSNVH GDAILPGMLC AGFLEGGTDA CQGDSGGPLV
560 570 580 590 597
CEEGTAEHQL TLRGVISWGS GCGDRNKPGV YTDVANYLAW IQKHIAS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3541 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
DAPDGPTVVLTVDGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
45
CPTAC ID
non-CPTAC-3541
Peptide Molecular Mass
1,510.7627
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 3.6 3.2 2.5 11.4 3.2 6.7 12 4.5 7.2 15 15 15
y2 (1+) 4.9 2.9 2.8 19 7.9 11.5 19.6 8.4 11.8 15 15 15
y6 (1+) 6.6 5.3 4.2 10.9 4.9 9.7 12.7 7.2 10.6 15 15 15
y7 (1+) 4.4 3.2 2.2 15.7 4.4 4.7 16.3 5.4 5.2 15 15 15
y10 (1+) 6.2 5.4 4.6 12.2 8 12.1 13.7 9.7 12.9 15 15 15
sum 3.1 1.6 1.7 12 3 6 12.4 3.4 6.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2650 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
EQPPSLTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
346
Peptide End
353
CPTAC ID
non-CPTAC-2650
Peptide Molecular Mass
926.4821
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 10.1 7 4.7 15 9.7 6.2 18.1 12 7.8 15 15 15
b2 (1+) 19.6 5.1 6.8 27.5 10.1 8 33.8 11.3 10.5 15 15 15
y5 (2+) 25.2 9.7 8.2 41 11.6 10.4 48.1 15.1 13.2 15 15 15
y6 (1+) 12.5 5.8 6.9 14 10.1 8.1 18.8 11.6 10.6 15 15 15
y5 (1+) 13.8 5.7 6.8 16.5 7.3 8.3 21.5 9.3 10.7 15 15 15
sum 7.4 5.4 3.9 13.5 8.8 5.9 15.4 10.3 7.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3707 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
TGPGGVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
153
Peptide End
160
CPTAC ID
non-CPTAC-3707
Peptide Molecular Mass
713.3820
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 8.4 7.9 8.1 17 11.6 8.1 19 14 11.5 15 15 15
y4 (1+) 31.3 26.8 13.6 36.5 35.3 19.8 48.1 44.3 24 15 15 15
y5 (1+) 8.6 14.3 5.2 21.4 13.8 8.5 23.1 19.9 10 15 15 15
y6 (1+) 16.7 14 7.4 14.9 18 11.9 22.4 22.8 14 15 15 15
y7 (1+) 25.7 57.4 10.1 24.4 46.1 20.3 35.4 73.6 22.7 15 15 15
sum 5.5 5 5.4 16.5 10.5 5.1 17.4 11.6 7.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1100 Collapse assay details

Data source: Panorama

Official Gene Symbol
F12
Peptide Sequence
VVGGLVALR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
373
Peptide End
381
CPTAC ID
non-CPTAC-1100
Peptide Molecular Mass
882.5651
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 10.9 6.2 4.9 18.5 6.6 5.9 21.5 9.1 7.7 15 15 15
y7 (1+) 5.8 3.6 5.1 10.8 7.6 6 12.3 8.4 7.9 15 15 15
y8 (1+) 17.4 4.5 4.3 15.3 4.4 5.7 23.2 6.3 7.1 15 15 15
sum 5 3.4 4.3 10.8 6.9 4.7 11.9 7.7 6.4 15 15 15


Additional Resources and Comments