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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fgl1 Mfire1
Sequence Length (AA) Molecular Weight (Da)
314 36439
Protein Name
Fibrinogen-like protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGKIYSFVLV AIALMMGREG WALESESCLR EQVRLRAQVH QLETRVKQQQ
60 70 80 90 100
TMIAQLLHEK EVQFLDKGSE NSFIDLGGKR QYADCSEIYN DGFKQSGFYK
110 120 130 140 150
IKPLQSLAEF SVYCDMSDGG GWTVIQRRSD GSENFNRGWN DYENGFGNFV
160 170 180 190 200
QNNGEYWLGN KNINLLTIQG DYTLKIDLTD FEKNSSFAQY QSFKVGDKKS
210 220 230 240 250
FYELNIGEYS GTAGDSLSGT FHPEVQWWAS HQRMKFSTWD RDNDNYQGNC
260 270 280 290 300
AEEEQSGWWF NRCHSANLNG VYYRGSYRAE TDNGVVWYTW HGWWYSLKSV
310 314
VMKIRPSDFI PNII

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3708 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fgl1
Peptide Sequence
IDLTDFEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
176
Peptide End
183
CPTAC ID
non-CPTAC-3708
Peptide Molecular Mass
979.4862
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 9.7 5.3 2 15.3 6.9 4.7 18.1 8.7 5.1 15 15 15
y3 (1+) 31.6 9.4 6.3 36.2 8.4 7.1 48.1 12.6 9.5 15 15 15
y5 (1+) 27.7 6.9 3.8 30.1 7.3 5.4 40.9 10 6.6 15 15 15
y6 (1+) 13.2 3.7 3.1 19.6 5.1 3.7 23.6 6.3 4.8 15 15 15
y7 (1+) 15.1 4.1 4.6 15.8 4.9 6.1 21.9 6.4 7.6 15 15 15
sum 9.1 2.8 1.9 12.2 4.5 3.8 15.2 5.3 4.2 15 15 15


Additional Resources and Comments